################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 688 GGTACC G^GTACC AseI 613 ATTAAT AT^TAAT AsnI 613 ATTAAT AT^TAAT Asp718I 688 GGTACC G^GTACC AspHI 2359 GTGCTC G[AT]GC[AT]^C BalI 1745 TGGCCA TGG^CCA BanI 688 GGTACC G^G[CT][GA]CC BciVI 2265 GGATAC GTATCC(6/5) BsgI 683 GTGCAG GTGCAG(16/14) BsiHKAI 2359 GTGCTC G[AT]GC[AT]^C BsmFI 30 GTCCC GGGAC(10/14) BssSI 1940 CACGAG CACGAG(-5/-1) BstXI 2169 CCAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 49 GCGC GCG^C DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT] EarI 989 CTCTTC CTCTTC(1/4) EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 296 CCTTGG C^C[AT][AT]GG FauI 115 GCGGG CCCGC(4/6) HgaI 1861 GACGC GACGC(5/10) HhaI 49 GCGC GCG^C HinP1I 49 GCGC G^CGC HindIII 151 AAGCTT A^AGCTT KpnI 688 GGTACC GGTAC^C Ksp632I 989 CTCTTC CTCTTC(1/4) MaeII 284 ACGT A^CGT MluNI 1745 TGGCCA TGG^CCA MscI 1745 TGGCCA TGG^CCA MspA1I 1930 CAGCTG C[AC]G^C[GT]G NaeI 10 GCCGGC GCC^GGC NgoMIV 10 GCCGGC G^CCGGC PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 613 ATTAAT AT^TAAT PvuII 1930 CAGCTG CAG^CTG ScaI 165 AGTACT AGT^ACT SpeI 60 ACTAGT A^CTAGT StyI 296 CCTTGG C^C[AT][AT]GG SwaI 1295 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Asp700I 302 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1370 GAATGGATTC BclI 180 TGATCA T^GATCA 2490 TGATCA BpmI 833 CTGGAG CTGGAG(16/14) 2177 CTGGAG BsaBI 2383 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2686 GATTCCAATC BsiEI 15 CGGCCG CG[GA][CT]^CG 74 CGGCCG BsmI 1026 GAATGC GAATGC(1/-1) 2172 GAATGC BspHI 1113 TCATGA T^CATGA 1359 TCATGA BsrFI 10 GCCGGC [GA]^CCGG[CT] 573 ACCGGC Cfr10I 10 GCCGGC [GA]^CCGG[CT] 573 ACCGGC EagI 15 CGGCCG C^GGCCG 74 CGGCCG EclXI 15 CGGCCG C^GGCCG 74 CGGCCG Eco52I 15 CGGCCG C^GGCCG 74 CGGCCG FbaI 180 TGATCA T^GATCA 2490 TGATCA MamI 2383 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2686 GATTCCAATC PflMI 1966 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2065 CCATCCAATGG RcaI 1113 TCATGA T^CATGA 1359 TCATGA SspI 738 AATATT AAT^ATT 823 AATATT Tsp45I 281 GTGAC ^GT[GC]AC 814 GTGAC Van91I 1966 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2065 CCATCCAATGG XcmI 1733 CCAGAGATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1962 CCACCCATTTTTTGG XmnI 302 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1370 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII