Restriction map for pF1KB5599
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraIII      DrdI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       Hin1I       
HincII      HindII      HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SphI        Sse8387I    StuI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        688  GGTACC               G^GTACC
AseI          613  ATTAAT               AT^TAAT
AsnI          613  ATTAAT               AT^TAAT
Asp718I       688  GGTACC               G^GTACC
AspHI        2359  GTGCTC               G[AT]GC[AT]^C
BalI         1745  TGGCCA               TGG^CCA
BanI          688  GGTACC               G^G[CT][GA]CC
BciVI        2265  GGATAC               GTATCC(6/5)
BsgI          683  GTGCAG               GTGCAG(16/14)
BsiHKAI      2359  GTGCTC               G[AT]GC[AT]^C
BsmFI          30  GTCCC                GGGAC(10/14)
BssSI        1940  CACGAG               CACGAG(-5/-1)
BstXI        2169  CCAGAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           49  GCGC                 GCG^C
DraII          28  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          989  CTCTTC               CTCTTC(1/4)
EcoO109I       28  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       296  CCTTGG               C^C[AT][AT]GG
FauI          115  GCGGG                CCCGC(4/6)
HgaI         1861  GACGC                GACGC(5/10)
HhaI           49  GCGC                 GCG^C
HinP1I         49  GCGC                 G^CGC
HindIII       151  AAGCTT               A^AGCTT
KpnI          688  GGTACC               GGTAC^C
Ksp632I       989  CTCTTC               CTCTTC(1/4)
MaeII         284  ACGT                 A^CGT
MluNI        1745  TGGCCA               TGG^CCA
MscI         1745  TGGCCA               TGG^CCA
MspA1I       1930  CAGCTG               C[AC]G^C[GT]G
NaeI           10  GCCGGC               GCC^GGC
NgoMIV         10  GCCGGC               G^CCGGC
PpuMI          28  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI         613  ATTAAT               AT^TAAT
PvuII        1930  CAGCTG               CAG^CTG
ScaI          165  AGTACT               AGT^ACT
SpeI           60  ACTAGT               A^CTAGT
StyI          296  CCTTGG               C^C[AT][AT]GG
SwaI         1295  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Asp700I       302  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1370  GAATGGATTC          
BclI          180  TGATCA               T^GATCA
             2490  TGATCA              
BpmI          833  CTGGAG               CTGGAG(16/14)
             2177  CTGGAG              
BsaBI        2383  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2686  GATTCCAATC          
BsiEI          15  CGGCCG               CG[GA][CT]^CG
               74  CGGCCG              
BsmI         1026  GAATGC               GAATGC(1/-1)
             2172  GAATGC              
BspHI        1113  TCATGA               T^CATGA
             1359  TCATGA              
BsrFI          10  GCCGGC               [GA]^CCGG[CT]
              573  ACCGGC              
Cfr10I         10  GCCGGC               [GA]^CCGG[CT]
              573  ACCGGC              
EagI           15  CGGCCG               C^GGCCG
               74  CGGCCG              
EclXI          15  CGGCCG               C^GGCCG
               74  CGGCCG              
Eco52I         15  CGGCCG               C^GGCCG
               74  CGGCCG              
FbaI          180  TGATCA               T^GATCA
             2490  TGATCA              
MamI         2383  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2686  GATTCCAATC          
PflMI        1966  CCATTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2065  CCATCCAATGG         
RcaI         1113  TCATGA               T^CATGA
             1359  TCATGA              
SspI          738  AATATT               AAT^ATT
              823  AATATT              
Tsp45I        281  GTGAC                ^GT[GC]AC
              814  GTGAC               
Van91I       1966  CCATTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2065  CCATCCAATGG         
XcmI         1733  CCAGAGATTTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1962  CCACCCATTTTTTGG     
XmnI          302  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1370  GAATGGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    Bsp1407I    BspMI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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