Restriction map for
pF1KB5631
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp700I AspEI AspI AviII
AvrII BalI BbeI BbrPI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BseAI BsiEI
BsiWI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 288 GGTACC G^GTACC
AccII 89 CGCG CG^CG
AflII 1566 CTTAAG C^TTAAG
Aor51HI 1982 AGCGCT AGC^GCT
AseI 298 ATTAAT AT^TAAT
AsnI 298 ATTAAT AT^TAAT
Asp718I 288 GGTACC G^GTACC
BbvCI 2983 GCTGAGG CCTCAGC(-5/-2)
BcgI 2280 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1566 CTTAAG C^TTAAG
BglII 685 AGATCT A^GATCT
BpmI 689 CTGGAG CTGGAG(16/14)
BsaBI 1125 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2891 TCCGGT [AT]^CCGG[AT]
BsmBI 1520 CGTCTC CGTCTC(1/5)
BspDI 1013 ATCGAT AT^CGAT
BsrBI 2121 CCGCTC CCGCTC(-3/-3)
BssSI 1955 CTCGTG CACGAG(-5/-1)
BstAPI 1704 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2365 TTCGAA TT^CGAA
BstUI 89 CGCG CG^CG
BstXI 981 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1983 GCGC GCG^C
ClaI 1013 ATCGAT AT^CGAT
EaeI 2730 TGGCCG [CT]^GGCC[GA]
Eco47III 1982 AGCGCT AGC^GCT
EcoNI 1448 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2078 GATATC GAT^ATC
HaeII 1982 AGCGCT [GA]GCGC^[CT]
HhaI 1983 GCGC GCG^C
HinP1I 1983 GCGC G^CGC
HindIII 1396 AAGCTT A^AGCTT
KpnI 288 GGTACC GGTAC^C
MamI 1125 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 89 CGCG CG^CG
NcoI 3092 CCATGG C^CATGG
NdeI 1025 CATATG CA^TATG
NspV 2365 TTCGAA TT^CGAA
PaeR7I 470 CTCGAG C^TCGAG
PshBI 298 ATTAAT AT^TAAT
SacI 2533 GAGCTC GAGCT^C
SapI 731 GAAGAGC GCTCTTC(1/4)
SfuI 2365 TTCGAA TT^CGAA
SphI 2639 GCATGC GCATG^C
SwaI 333 ATTTAAAT ATTT^AAAT
XcmI 3083 CCATAGTGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 470 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 128 ACGTGT A^C[GA][CT]GT
437 ACACGT
AlwNI 1809 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2687 CAGAGACTG
AspHI 1471 GAGCAC G[AT]GC[AT]^C
2533 GAGCTC
AvaI 470 CTCGAG C^[CT]CG[GA]G
2741 CCCGAG
BamHI 2297 GGATCC G^GATCC
2811 GGATCC
BanII 1382 GGGCTC G[GA]GC[CT]^C
2533 GAGCTC
BbsI 431 GAAGAC GAAGAC(2/6)
1843 GTCTTC
BfaI 428 CTAG C^TAG
2548 CTAG
BpuAI 431 GAAGAC GAAGAC(2/6)
1843 GTCTTC
BsiHKAI 1471 GAGCAC G[AT]GC[AT]^C
2533 GAGCTC
BsmI 1044 GAATGC GAATGC(1/-1)
1208 GCATTC
BsoBI 470 CTCGAG C^[CT]CG[GA]G
2741 CCCGAG
BspMI 1539 ACCTGC ACCTGC(4/8)
1709 ACCTGC
BsrFI 2383 ACCGGC [GA]^CCGG[CT]
2732 GCCGGT
Cfr10I 2383 ACCGGC [GA]^CCGG[CT]
2732 GCCGGT
DraI 334 TTTAAA TTT^AAA
921 TTTAAA
EcoRI 841 GAATTC G^AATTC
973 GAATTC
FauI 90 GCGGG CCCGC(4/6)
1503 GCGGG
HgaI 1717 GACGC GACGC(5/10)
1317 GCGTC
HphI 1338 GGTGA GGTGA(8/7)
1386 TCACC
MaeI 428 CTAG C^TAG
2548 CTAG
MslI 1332 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1482 CACAGACGTG
PstI 40 CTGCAG CTGCA^G
1934 CTGCAG
PvuII 628 CAGCTG CAG^CTG
2929 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BanI BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII