Restriction map for
pF1KB5639
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspMI BsrDI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI Ksp632I MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp509I Van91I
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 902 ACATGT A^C[GA][CT]GT
AlwI 997 GATCC GGATC(4/5)
ApaI 38 GGGCCC GGGCC^C
AspI 805 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 913 CCCGGG C^[CT]CG[GA]G
BbsI 265 GAAGAC GAAGAC(2/6)
BcgI 1026 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 164 TGATCA T^GATCA
BpuAI 265 GAAGAC GAAGAC(2/6)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsoBI 913 CCCGGG C^[CT]CG[GA]G
BspHI 1070 TCATGA T^CATGA
BspLU11I 902 ACATGT A^CATGT
BsrFI 939 ACCGGC [GA]^CCGG[CT]
BsrI 317 ACTGG ACTGG(1/-1)
BstEII 591 GGTGACC G^GT[ACGT]ACC
BstPI 591 GGTGACC G^GT[ACGT]ACC
BstXI 482 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 939 ACCGGC [GA]^CCGG[CT]
DsaI 128 CCGCGG C^C[GA][CT]GG
EaeI 26 CGGCCG [CT]^GGCC[GA]
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoO65I 591 GGTGACC G^GT[ACGT]ACC
EcoT14I 1214 CCTTGG C^C[AT][AT]GG
FbaI 164 TGATCA T^GATCA
HgaI 1221 GACGC GACGC(5/10)
KspI 128 CCGCGG CCGC^GG
MslI 316 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 902 ACATGT [GA]CATG^[CT]
PflFI 805 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1070 TCATGA T^CATGA
SacI 1269 GAGCTC GAGCT^C
SacII 128 CCGCGG CCGC^GG
SexAI 349 ACCAGGT A^CC[AT]GGT
SfcI 306 CTATAG C^T[GA][CT]AG
SmaI 913 CCCGGG CCC^GGG
StuI 85 AGGCCT AGG^CCT
StyI 1214 CCTTGG C^C[AT][AT]GG
Tth111I 805 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1290 TCTAGA T^CTAGA
XmaCI 913 CCCGGG C^CCGGG
XmaI 913 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 74 CGCG CG^CG
129 CGCG
AlwNI 944 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1304 CAGACTCTG
AspHI 115 GTGCTC G[AT]GC[AT]^C
1269 GAGCTC
BanI 424 GGCACC G^G[CT][GA]CC
1044 GGTGCC
BbvCI 799 GCTGAGG CCTCAGC(-5/-2)
1117 GCTGAGG
BfaI 181 CTAG C^TAG
1291 CTAG
BpmI 597 CTCCAG CTGGAG(16/14)
516 CTGGAG
BsaI 144 GGTCTC GGTCTC(1/5)
956 GGTCTC
BsgI 345 CTGCAC GTGCAG(16/14)
384 CTGCAC
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
1269 GAGCTC
BsmBI 586 GAGACG CGTCTC(1/5)
1219 GAGACG
Bsp1407I 508 TGTACA T^GTACA
1145 TGTACA
BsrBI 1066 CCGCTC CCGCTC(-3/-3)
197 GAGCGG
BsrGI 508 TGTACA T^GTACA
1145 TGTACA
BstAPI 281 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1303 GCAGACTCTGC
BstF5I 488 GGATG GGATG(2/0)
1209 GGATG
BstUI 74 CGCG CG^CG
129 CGCG
CfoI 73 GCGC GCG^C
342 GCGC
FauI 75 GCGGG CCCGC(4/6)
1126 GCGGG
FokI 488 GGATG GGATG(9/13)
1209 GGATG
HhaI 73 GCGC GCG^C
342 GCGC
HinP1I 73 GCGC G^CGC
342 GCGC
HincII 898 GTCAAC GT[CT]^[GA]AC
1168 GTCAAC
HindII 898 GTCAAC GT[CT]^[GA]AC
1168 GTCAAC
MaeI 181 CTAG C^TAG
1291 CTAG
MvnI 74 CGCG CG^CG
129 CGCG
PvuII 149 CAGCTG CAG^CTG
575 CAGCTG
SspBI 508 TGTACA T^GTACA
1145 TGTACA
TaqI 527 TCGA T^CGA
1025 TCGA
TthHB8I 527 TCGA T^CGA
1025 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII DraIII EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I TspRI