Restriction map for
pF1KB5640
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BbrPI BbvCI BcgI BfrI BglII
BlnI BsaAI BsaWI BseAI BsiEI
BsiHKAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MflI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 976 GTCTAC GT^[AC][GT]AC
AcyI 263 GGCGCC G[GA]^CG[CT]C
AflIII 151 ACATGT A^C[GA][CT]GT
AlwI 1391 GGATC GGATC(4/5)
ApaI 709 GGGCCC GGGCC^C
AspI 478 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 167 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BbeI 263 GGCGCC GGCGC^C
BciVI 360 GGATAC GTATCC(6/5)
BfaI 1465 CTAG C^TAG
BglI 334 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1148 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1148 GCTCAGC GC^T[ACGT]AGC
BsaBI 99 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 263 GGCGCC G[GA]^CG[CT]C
BsaI 1031 GAGACC GGTCTC(1/5)
BsmBI 352 GAGACG CGTCTC(1/5)
BsmI 1138 GAATGC GAATGC(1/-1)
BsoBI 167 CTCGGG C^[CT]CG[GA]G
BspLU11I 151 ACATGT A^CATGT
BspMI 972 GCAGGT ACCTGC(4/8)
BstAPI 232 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1148 GCTCAGC GC^T[ACGT]AGC
DraI 1438 TTTAAA TTT^AAA
DraII 1059 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 1171 CTCTTC CTCTTC(1/4)
EcoNI 242 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1059 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 263 GGCGCC [GA]GCGC^[CT]
Hin1I 263 GGCGCC G[GA]^CG[CT]C
KasI 263 GGCGCC G^GCGCC
Ksp632I 1171 CTCTTC CTCTTC(1/4)
MaeI 1465 CTAG C^TAG
MamI 99 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 1491 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 304 CAGCTG C[AC]G^C[GT]G
NarI 263 GGCGCC GG^CGCC
PflFI 478 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1426 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 304 CAGCTG CAG^CTG
SapI 1170 GCTCTTC GCTCTTC(1/4)
SexAI 314 ACCTGGT A^CC[AT]GGT
SfoI 263 GGCGCC GGC^GCC
Tsp509I 729 AATT ^AATT
Tth111I 478 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1426 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 62 CGCG CG^CG
331 CGCG
AvaII 327 GGACC G^G[AT]CC
603 GGTCC
BanI 263 GGCGCC G^G[CT][GA]CC
573 GGCACC
BbsI 1159 GAAGAC GAAGAC(2/6)
1189 GTCTTC
BclI 656 TGATCA T^GATCA
1071 TGATCA
BcnI 668 CCGGG CC^[GC]GG
809 CCCGG
BpuAI 1159 GAAGAC GAAGAC(2/6)
1189 GTCTTC
BseRI 967 GAGGAG GAGGAG(10/8)
1228 GAGGAG
BsgI 230 CTGCAC GTGCAG(16/14)
298 CTGCAC
BsmAI 352 GAGAC GTCTC(1/5)
1031 GAGAC
BsmFI 326 GGGAC GGGAC(10/14)
807 GTCCC
BstUI 62 CGCG CG^CG
331 CGCG
FbaI 656 TGATCA T^GATCA
1071 TGATCA
MaeII 209 ACGT A^CGT
482 ACGT
MseI 794 TTAA T^TAA
1439 TTAA
MvnI 62 CGCG CG^CG
331 CGCG
NaeI 51 GCCGGC GCC^GGC
266 GCCGGC
NciI 668 CCGGG CC^[GC]GG
809 CCCGG
NgoMIV 51 GCCGGC G^CCGGC
266 GCCGGC
NspI 151 ACATGT [GA]CATG^[CT]
1211 ACATGC
TfiI 124 GAATC G^A[AT]TC
704 GATTC
Tru9I 794 TTAA T^TAA
1439 TTAA
XcmI 68 CCATCCGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
953 CCAGGGTGTTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I TspRI TthHB8I