Restriction map for
pF1KB5648
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccIII AclI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI BmyI Bpu1102I
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HgaI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MunI NciI NcoI NdeI NheI
NotI NruI NsiI PacI PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SmaI SnaBI SphI Sse8387I
SspBI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AccII 18 CGCG CG^CG
AcyI 7 GGCGCC G[GA]^CG[CT]C
AflII 1168 CTTAAG C^TTAAG
AgeI 1086 ACCGGT A^CCGGT
AhdI 1491 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2104 CCTGAGG CC^T[ACGT]AGG
Asp700I 673 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1491 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 65 CTCGAG C^[CT]CG[GA]G
BanI 7 GGCGCC G^G[CT][GA]CC
BbeI 7 GGCGCC GGCGC^C
BclI 852 TGATCA T^GATCA
BfrI 1168 CTTAAG C^TTAAG
BglII 79 AGATCT A^GATCT
BpmI 1211 CTGGAG CTGGAG(16/14)
BsaAI 595 CACGTA [CT]AC^GT[GA]
BsaBI 2270 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 7 GGCGCC G[GA]^CG[CT]C
BsaWI 1086 ACCGGT [AT]^CCGG[AT]
BsmFI 1544 GTCCC GGGAC(10/14)
BsmI 2142 GAATGC GAATGC(1/-1)
BsoBI 65 CTCGAG C^[CT]CG[GA]G
BspHI 873 TCATGA T^CATGA
BspMI 652 ACCTGC ACCTGC(4/8)
BsrBI 816 CCGCTC CCGCTC(-3/-3)
BsrDI 1345 GCAATG GCAATG(2/0)
BssKI 741 CCTGG ^CC[ACGT]GG
BstBI 1385 TTCGAA TT^CGAA
BstNI 741 CCTGG CC^[AT]GG
BstUI 18 CGCG CG^CG
BstYI 79 AGATCT [GA]^GATC[CT]
Bsu36I 2104 CCTGAGG CC^T[ACGT]AGG
Cfr13I 1200 GGCCC G^G[ACGT]CC
DraIII 388 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1491 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2104 CCTGAGG CC^T[ACGT]AGG
EcoRII 741 CCTGG ^CC[AT]GG
EcoT14I 284 CCTTGG C^C[AT][AT]GG
FbaI 852 TGATCA T^GATCA
HaeII 7 GGCGCC [GA]GCGC^[CT]
Hin1I 7 GGCGCC G[GA]^CG[CT]C
HindIII 495 AAGCTT A^AGCTT
HpaI 2473 GTTAAC GTT^AAC
KasI 7 GGCGCC G^GCGCC
MamI 2270 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 79 AGATCT [GA]^GATC[CT]
MvaI 741 CCTGG CC^[AT]GG
MvnI 18 CGCG CG^CG
NaeI 10 GCCGGC GCC^GGC
NarI 7 GGCGCC GG^CGCC
NgoMIV 10 GCCGGC G^CCGGC
NspI 2335 GCATGT [GA]CATG^[CT]
NspV 1385 TTCGAA TT^CGAA
PaeR7I 65 CTCGAG C^TCGAG
PflMI 1204 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1086 ACCGGT A^CCGGT
PstI 2437 CTGCAG CTGCA^G
PvuII 2380 CAGCTG CAG^CTG
RcaI 873 TCATGA T^CATGA
SalI 731 GTCGAC G^TCGAC
Sau96I 1200 GGCCC G^G[ACGT]CC
ScrFI 741 CCTGG CC^[ACGT]GG
SfoI 7 GGCGCC GGC^GCC
SfuI 1385 TTCGAA TT^CGAA
SgrAI 9 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1954 ACTAGT A^CTAGT
StuI 167 AGGCCT AGG^CCT
StyI 284 CCTTGG C^C[AT][AT]GG
Van91I 1204 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 618 CCAACCTCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 65 CTCGAG C^TCGAG
XhoII 79 AGATCT [GA]^GATC[CT]
XmnI 673 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 731 GTCGAC GT^[AC][GT]AC
2339 GTAGAC
AflIII 2066 ACGTGT A^C[GA][CT]GT
2259 ACGTGT
AlwI 1485 GGATC GGATC(4/5)
2466 GGATC
BbsI 1624 GAAGAC GAAGAC(2/6)
1897 GAAGAC
BpuAI 1624 GAAGAC GAAGAC(2/6)
1897 GAAGAC
BsaJI 284 CCTTGG C^C[ACGT][ACGT]GG
531 CCGAGG
BsgI 579 CTGCAC GTGCAG(16/14)
1403 CTGCAC
BsrFI 10 GCCGGC [GA]^CCGG[CT]
1086 ACCGGT
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
1086 ACCGGT
EcoNI 2413 CCTATACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2433 CCTACTGCAGG
EcoRI 984 GAATTC G^AATTC
1588 GAATTC
HapII 11 CCGG C^CGG
1087 CCGG
HincII 731 GTCGAC GT[CT]^[GA]AC
2473 GTTAAC
HindII 731 GTCGAC GT[CT]^[GA]AC
2473 GTTAAC
HpaII 11 CCGG C^CGG
1087 CCGG
MspI 11 CCGG C^CGG
1087 CCGG
SfcI 2096 CTACAG C^T[GA][CT]AG
2437 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BfaI BsiYI BslI
BsmAI BsrI BstF5I Cac8I CfoI
DdeI DpnII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI SfaNI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I