Restriction map for
pF1KB5649
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 463 GGTACC G^GTACC
AccI 736 GTCTAC GT^[AC][GT]AC
AclI 28 AACGTT AA^CGTT
AcsI 1489 AAATTT [GA]^AATT[CT]
AflII 985 CTTAAG C^TTAAG
AflIII 873 ACATGT A^C[GA][CT]GT
AlwNI 2038 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 578 CCTTAGG CC^T[ACGT]AGG
ApoI 1489 AAATTT [GA]^AATT[CT]
Asp718I 463 GGTACC G^GTACC
AvaII 1398 GGTCC G^G[AT]CC
BalI 399 TGGCCA TGG^CCA
BfrI 985 CTTAAG C^TTAAG
BpmI 686 CTGGAG CTGGAG(16/14)
BsaI 127 GAGACC GGTCTC(1/5)
BsmI 1777 GAATGC GAATGC(1/-1)
Bsp1407I 422 TGTACA T^GTACA
BspLU11I 873 ACATGT A^CATGT
BsrDI 378 CATTGC GCAATG(2/0)
BsrGI 422 TGTACA T^GTACA
BstAPI 1312 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 578 CCTTAGG CC^T[ACGT]AGG
Cfr13I 1398 GGTCC G^G[ACGT]CC
DraI 1783 TTTAAA TTT^AAA
EaeI 399 TGGCCA [CT]^GGCC[GA]
Eco81I 578 CCTTAGG CC^T[ACGT]AGG
EcoNI 574 CCTTCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 171 GCGTC GACGC(5/10)
HindIII 982 AAGCTT A^AGCTT
KpnI 463 GGTACC GGTAC^C
MaeII 29 ACGT A^CGT
MfeI 1460 CAATTG C^AATTG
MluNI 399 TGGCCA TGG^CCA
MscI 399 TGGCCA TGG^CCA
MslI 477 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 47 CAGCTG C[AC]G^C[GT]G
MunI 1460 CAATTG C^AATTG
NspI 873 ACATGT [GA]CATG^[CT]
PflMI 1505 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 28 AACGTT AA^CGTT
PvuII 47 CAGCTG CAG^CTG
Sau96I 1398 GGTCC G^G[ACGT]CC
SmlI 985 CTTAAG C^T[CT][GA]AG
SpeI 1306 ACTAGT A^CTAGT
SspBI 422 TGTACA T^GTACA
SspI 2093 AATATT AAT^ATT
StuI 1587 AGGCCT AGG^CCT
Van91I 1505 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 1632 CCGC CCGC(-3/-1)
1743 CCGC
Asp700I 584 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2113 GAAAACCTTC
AspHI 569 GTGCTC G[AT]GC[AT]^C
600 GAGCAC
BbsI 3 GTCTTC GAAGAC(2/6)
877 GTCTTC
BciVI 869 GGATAC GTATCC(6/5)
1530 GGATAC
BclI 135 TGATCA T^GATCA
1454 TGATCA
BpuAI 3 GTCTTC GAAGAC(2/6)
877 GTCTTC
BsiHKAI 569 GTGCTC G[AT]GC[AT]^C
600 GAGCAC
EarI 2122 CTCTTC CTCTTC(1/4)
1672 GAAGAG
EcoT14I 1446 CCTTGG C^C[AT][AT]GG
1928 CCTTGG
FbaI 135 TGATCA T^GATCA
1454 TGATCA
HincII 526 GTTGAC GT[CT]^[GA]AC
1810 GTCAAC
HindII 526 GTTGAC GT[CT]^[GA]AC
1810 GTCAAC
Ksp632I 2122 CTCTTC CTCTTC(1/4)
1672 GAAGAG
PstI 220 CTGCAG CTGCA^G
1956 CTGCAG
StyI 1446 CCTTGG C^C[AT][AT]GG
1928 CCTTGG
XmnI 584 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2113 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AfaI AluI AlwI BamHI BanI
BanII BbvI BfaI BmyI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScaI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII