Restriction map for
pF1KB5655
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI Aor51HI ApaI AscI Asp700I
Asp718I AspI AviII AvrII BalI
BanI BbeI BglI BlnI BsaBI
BsaHI BsaI BseAI BsiWI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstUI BstXI BstZ17I CfoI CpoI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoT22I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PmeI Psp1406I RcaI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccI 1638 GTCTAC GT^[AC][GT]AC
AciI 187 GCGG CCGC(-3/-1)
AgeI 721 ACCGGT A^CCGGT
AhdI 2590 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 266 GTGCAC G^TGCAC
AlwNI 1598 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 266 GTGCAC G^TGCAC
AseI 1147 ATTAAT AT^TAAT
AsnI 1147 ATTAAT AT^TAAT
AspEI 2590 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 173 CTCGGG C^[CT]CG[GA]G
BamHI 727 GGATCC G^GATCC
BbrPI 3162 CACGTG CAC^GTG
BcgI 1338 GCAAGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 820 GGATAC GTATCC(6/5)
BclI 975 TGATCA T^GATCA
BcnI 555 CCGGG CC^[GC]GG
BglII 742 AGATCT A^GATCT
BlpI 919 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 919 GCTGAGC GC^T[ACGT]AGC
BsaWI 721 ACCGGT [AT]^CCGG[AT]
BsgI 3225 CTGCAC GTGCAG(16/14)
BsiEI 2962 CGATCG CG[GA][CT]^CG
BsmBI 1734 CGTCTC CGTCTC(1/5)
BsmFI 691 GTCCC GGGAC(10/14)
BsoBI 173 CTCGGG C^[CT]CG[GA]G
Bsp1407I 639 TGTACA T^GTACA
BspDI 2960 ATCGAT AT^CGAT
BsrFI 721 ACCGGT [GA]^CCGG[CT]
BsrGI 639 TGTACA T^GTACA
BstBI 604 TTCGAA TT^CGAA
BstEII 946 GGTGACC G^GT[ACGT]ACC
BstPI 946 GGTGACC G^GT[ACGT]ACC
CelII 919 GCTGAGC GC^T[ACGT]AGC
Cfr10I 721 ACCGGT [GA]^CCGG[CT]
ClaI 2960 ATCGAT AT^CGAT
DraIII 1384 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 673 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2590 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 946 GGTGACC G^GT[ACGT]ACC
EcoRI 3413 GAATTC G^AATTC
EcoRV 753 GATATC GAT^ATC
EcoT14I 3296 CCATGG C^C[AT][AT]GG
FbaI 975 TGATCA T^GATCA
HgaI 2613 GACGC GACGC(5/10)
HincII 839 GTCAAC GT[CT]^[GA]AC
HindII 839 GTCAAC GT[CT]^[GA]AC
MslI 3319 CATACGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 555 CCGGG CC^[GC]GG
NcoI 3296 CCATGG C^CATGG
NdeI 1991 CATATG CA^TATG
NheI 361 GCTAGC G^CTAGC
NspV 604 TTCGAA TT^CGAA
PflMI 3394 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 721 ACCGGT A^CCGGT
PmaCI 3162 CACGTG CAC^GTG
PmlI 3162 CACGTG CAC^GTG
PshAI 2361 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1147 ATTAAT AT^TAAT
PvuI 2962 CGATCG CGAT^CG
ScaI 2257 AGTACT AGT^ACT
SfuI 604 TTCGAA TT^CGAA
SnaBI 3321 TACGTA TAC^GTA
SpeI 2044 ACTAGT A^CTAGT
SspBI 639 TGTACA T^GTACA
StyI 3296 CCATGG C^C[AT][AT]GG
Van91I 3394 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1473 TCTAGA T^CTAGA
XcmI 659 CCACAGTGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflII 1819 CTTAAG C^TTAAG
3040 CTTAAG
AocI 88 CCTGAGG CC^T[ACGT]AGG
3115 CCTGAGG
BanII 530 GAGCCC G[GA]GC[CT]^C
848 GAGCCC
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
274 GCTGAGG
BfrI 1819 CTTAAG C^TTAAG
3040 CTTAAG
BsaAI 3162 CACGTG [CT]AC^GT[GA]
3321 TACGTA
BspMI 270 ACCTGC ACCTGC(4/8)
2419 GCAGGT
BsrDI 3017 CATTGC GCAATG(2/0)
994 GCAATG
BstAPI 156 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3304 GCATTTTTTGC
Bsu36I 88 CCTGAGG CC^T[ACGT]AGG
3115 CCTGAGG
DraI 1183 TTTAAA TTT^AAA
1807 TTTAAA
DraII 705 AGGACCT [GA]G^G[ACGT]CC[CT]
1253 AGGACCT
DsaI 535 CCACGG C^C[GA][CT]GG
3296 CCATGG
Eco81I 88 CCTGAGG CC^T[ACGT]AGG
3115 CCTGAGG
EcoO109I 705 AGGACCT [GA]G^G[ACGT]CC[CT]
1253 AGGACCT
HindIII 3235 AAGCTT A^AGCTT
3505 AAGCTT
MspA1I 899 CAGCTG C[AC]G^C[GT]G
1598 CAGCTG
NlaIV 727 GGATCC GG[ACGT]^[ACGT]CC
2901 GGAACC
PpuMI 705 AGGACCT [GA]G^G[AT]CC[CT]
1253 AGGACCT
PstI 1012 CTGCAG CTGCA^G
3198 CTGCAG
PvuII 899 CAGCTG CAG^CTG
1598 CAGCTG
SapI 1224 GCTCTTC GCTCTTC(1/4)
2677 GCTCTTC
SspI 1030 AATATT AAT^ATT
3526 AATATT
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
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AcsI AfaI AflIII AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NspI PleI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII