################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BciVI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsoBI BspDI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI Tsp45I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 2531 GTAGAC GT^[AC][GT]AC AccIII 47 TCCGGA T^CCGGA AciI 1635 GCGG CCGC(-3/-1) AclI 323 AACGTT AA^CGTT AflIII 2261 ACATGT A^C[GA][CT]GT ApaI 144 GGGCCC GGGCC^C AseI 394 ATTAAT AT^TAAT AsnI 394 ATTAAT AT^TAAT AvaII 60 GGACC G^G[AT]CC BamHI 1806 GGATCC G^GATCC BclI 1154 TGATCA T^GATCA BcnI 2025 CCCGG CC^[GC]GG BlpI 1426 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1426 GCTCAGC GC^T[ACGT]AGC BsaWI 47 TCCGGA [AT]^CCGG[AT] BseAI 47 TCCGGA T^CCGGA BseRI 301 GAGGAG GAGGAG(10/8) BsmAI 1879 GAGAC GTCTC(1/5) BspEI 47 TCCGGA T^CCGGA BspHI 2355 TCATGA T^CATGA BspLU11I 2261 ACATGT A^CATGT BstAPI 2429 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1209 CCAGGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1426 GCTCAGC GC^T[ACGT]AGC DraII 143 AGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 210 CCGTGG C^C[GA][CT]GG EcoO109I 143 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 672 GAATTC G^AATTC EcoRV 364 GATATC GAT^ATC FbaI 1154 TGATCA T^GATCA MroI 47 TCCGGA T^CCGGA NciI 2025 CCCGG CC^[GC]GG PacI 391 TTAATTAA TTAAT^TAA PshBI 394 ATTAAT AT^TAAT Psp1406I 323 AACGTT AA^CGTT RcaI 2355 TCATGA T^CATGA SacI 187 GAGCTC GAGCT^C SapI 2192 GAAGAGC GCTCTTC(1/4) SspI 2074 AATATT AAT^ATT SwaI 1410 ATTTAAAT ATTT^AAAT XbaI 1170 TCTAGA T^CTAGA XcmI 1907 CCAGAGCACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 2093 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2704 CAGACACTG BanII 144 GGGCCC G[GA]GC[CT]^C 187 GAGCTC BbsI 51 GAAGAC GAAGAC(2/6) 1834 GAAGAC BcgI 2596 CGACAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1469 GCAAAGACCTCG BglII 594 AGATCT A^GATCT 1752 AGATCT BpuAI 51 GAAGAC GAAGAC(2/6) 1834 GAAGAC BsgI 112 GTGCAG GTGCAG(16/14) 452 GTGCAG Bsp1407I 710 TGTACA T^GTACA 2264 TGTACA BspMI 238 GCAGGT ACCTGC(4/8) 448 GCAGGT BsrGI 710 TGTACA T^GTACA 2264 TGTACA EcoNI 92 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1062 CCTTAAAAAGG EcoT14I 1268 CCTTGG C^C[AT][AT]GG 2444 CCTTGG EcoT22I 1557 ATGCAT ATGCA^T 2048 ATGCAT HapII 48 CCGG C^CGG 2026 CCGG HpaII 48 CCGG C^CGG 2026 CCGG MspI 48 CCGG C^CGG 2026 CCGG NsiI 1557 ATGCAT ATGCA^T 2048 ATGCAT SmlI 172 CTCAAG C^T[CT][GA]AG 598 CTTGAG SpeI 1129 ACTAGT A^CTAGT 2224 ACTAGT SphI 1555 GCATGC GCATG^C 1668 GCATGC SspBI 710 TGTACA T^GTACA 2264 TGTACA StyI 1268 CCTTGG C^C[AT][AT]GG 2444 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AspHI BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HgaI HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI