Restriction map for
pF1KB5679
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# NO CUT ENZYMES ( 116 enzymes) #
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AccI AccII AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII EaeI
EagI Eam1105I EclXI Eco52I EcoNI
FseI FspI HapII HgaI HindIII
HpaI HpaII KasI KspI MluI
MluNI MroI MscI MspI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1748 GGTACC G^GTACC
AflII 1171 CTTAAG C^TTAAG
AocI 1966 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1691 AGCGCT AGC^GCT
ApaI 1733 GGGCCC GGGCC^C
Asp700I 178 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1748 GGTACC G^GTACC
AspHI 2393 GTGCTC G[AT]GC[AT]^C
BanI 1748 GGTACC G^G[CT][GA]CC
BbsI 1717 GAAGAC GAAGAC(2/6)
BclI 2687 TGATCA T^GATCA
BfrI 1171 CTTAAG C^TTAAG
BglII 339 AGATCT A^GATCT
BpuAI 1717 GAAGAC GAAGAC(2/6)
BsaBI 2685 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1411 GAGGAG GAGGAG(10/8)
BsgI 2012 GTGCAG GTGCAG(16/14)
BsiHKAI 2393 GTGCTC G[AT]GC[AT]^C
BsmBI 1036 GAGACG CGTCTC(1/5)
BsmI 354 GAATGC GAATGC(1/-1)
BsrBI 2250 CCGCTC CCGCTC(-3/-3)
BssSI 1779 CACGAG CACGAG(-5/-1)
BstEII 1089 GGTCACC G^GT[ACGT]ACC
BstPI 1089 GGTCACC G^GT[ACGT]ACC
BstXI 1122 CCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1966 CCTAAGG CC^T[ACGT]AGG
DraI 2370 TTTAAA TTT^AAA
DrdI 235 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1004 CCATGG C^C[GA][CT]GG
Eco47III 1691 AGCGCT AGC^GCT
Eco81I 1966 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1089 GGTCACC G^GT[ACGT]ACC
FbaI 2687 TGATCA T^GATCA
HaeII 1691 AGCGCT [GA]GCGC^[CT]
HincII 2484 GTCAAC GT[CT]^[GA]AC
HindII 2484 GTCAAC GT[CT]^[GA]AC
KpnI 1748 GGTACC GGTAC^C
MamI 2685 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 722 CAATTG C^AATTG
MslI 2679 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 722 CAATTG C^AATTG
NcoI 1004 CCATGG C^CATGG
NdeI 2672 CATATG CA^TATG
PstI 1738 CTGCAG CTGCA^G
PvuII 1936 CAGCTG CAG^CTG
SbfI 1737 CCTGCAGG CCTGCA^GG
SexAI 1136 ACCTGGT A^CC[AT]GGT
Sse8387I 1737 CCTGCAGG CCTGCA^GG
SspI 2140 AATATT AAT^ATT
XcmI 813 CCACTCTCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 178 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 1038 GACGTC GACGT^C
2591 GACGTC
AcyI 1038 GACGTC G[GA]^CG[CT]C
2591 GACGTC
AvaII 1920 GGACC G^G[AT]CC
2639 GGACC
BamHI 144 GGATCC G^GATCC
1548 GGATCC
BsaHI 1038 GACGTC G[GA]^CG[CT]C
2591 GACGTC
BsmAI 1036 GAGAC GTCTC(1/5)
2019 GAGAC
BsmFI 1919 GGGAC GGGAC(10/14)
2237 GGGAC
BspHI 432 TCATGA T^CATGA
1989 TCATGA
BsrDI 16 GCAATG GCAATG(2/0)
305 GCAATG
CfoI 1117 GCGC GCG^C
1692 GCGC
EcoRI 575 GAATTC G^AATTC
1290 GAATTC
EcoRV 943 GATATC GAT^ATC
2149 GATATC
EcoT22I 96 ATGCAT ATGCA^T
860 ATGCAT
FauI 1298 CCCGC CCCGC(4/6)
2080 CCCGC
HhaI 1117 GCGC GCG^C
1692 GCGC
Hin1I 1038 GACGTC G[GA]^CG[CT]C
2591 GACGTC
HinP1I 1117 GCGC G^CGC
1692 GCGC
MaeII 1039 ACGT A^CGT
2592 ACGT
MspA1I 1316 CAGCGG C[AC]G^C[GT]G
1936 CAGCTG
NsiI 96 ATGCAT ATGCA^T
860 ATGCAT
NspI 704 ACATGC [GA]CATG^[CT]
1568 ACATGC
RcaI 432 TCATGA T^CATGA
1989 TCATGA
ScaI 935 AGTACT AGT^ACT
1439 AGTACT
SfcI 1458 CTATAG C^T[GA][CT]AG
1738 CTGCAG
TaqI 290 TCGA T^CGA
2090 TCGA
TthHB8I 290 TCGA T^CGA
2090 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII