Restriction map for
pF1KB5682
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 680 GTATAC GT^[AC][GT]AC
AccII 1215 CGCG CG^CG
AcsI 1577 AAATTC [GA]^AATT[CT]
AflIII 330 ACATGT A^C[GA][CT]GT
AhdI 4 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1259 GTGCAC G^TGCAC
AlwI 1417 GGATC GGATC(4/5)
ApaI 1592 GGGCCC GGGCC^C
ApaLI 1259 GTGCAC G^TGCAC
ApoI 1577 AAATTC [GA]^AATT[CT]
Asp700I 1612 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1199 GGTGCC G^G[CT][GA]CC
BbvCI 730 CCTCAGC CCTCAGC(-5/-2)
BciVI 1054 GGATAC GTATCC(6/5)
BglII 1283 AGATCT A^GATCT
BsaAI 1057 TACGTG [CT]AC^GT[GA]
BsaWI 1530 ACCGGA [AT]^CCGG[AT]
BseRI 1073 GAGGAG GAGGAG(10/8)
BsmBI 289 CGTCTC CGTCTC(1/5)
BsmI 925 GCATTC GAATGC(1/-1)
Bsp1407I 1224 TGTACA T^GTACA
BspLU11I 330 ACATGT A^CATGT
BsrDI 975 CATTGC GCAATG(2/0)
BsrGI 1224 TGTACA T^GTACA
Bst1107I 680 GTATAC GTA^TAC
BstUI 1215 CGCG CG^CG
BstXI 677 CCAGTATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1283 AGATCT [GA]^GATC[CT]
BstZ17I 680 GTATAC GTA^TAC
DrdI 1210 GACCACGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1391 CCGTGG C^C[GA][CT]GG
EaeI 855 CGGCCA [CT]^GGCC[GA]
Eam1105I 4 GACAATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1494 ATGCAT ATGCA^T
FauI 873 CCCGC CCCGC(4/6)
HgaI 1320 GCGTC GACGC(5/10)
HincII 1094 GTCAAC GT[CT]^[GA]AC
HindII 1094 GTCAAC GT[CT]^[GA]AC
MaeII 1058 ACGT A^CGT
MfeI 116 CAATTG C^AATTG
MflI 1283 AGATCT [GA]^GATC[CT]
MunI 116 CAATTG C^AATTG
MvnI 1215 CGCG CG^CG
NsiI 1494 ATGCAT ATGCA^T
NspI 330 ACATGT [GA]CATG^[CT]
PleI 613 GAGTC GAGTC(4/5)
PshAI 1480 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 194 GAGCTC GAGCT^C
ScaI 1614 AGTACT AGT^ACT
SspBI 1224 TGTACA T^GTACA
SspI 338 AATATT AAT^ATT
Tsp45I 1208 GTGAC ^GT[GC]AC
XhoII 1283 AGATCT [GA]^GATC[CT]
XmnI 1612 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 1225 GTAC GT^AC
1615 GTAC
AlwNI 1370 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1454 CAGCTGCTG
AspHI 194 GAGCTC G[AT]GC[AT]^C
1259 GTGCAC
AvaII 1062 GGACC G^G[AT]CC
1528 GGACC
BanII 194 GAGCTC G[GA]GC[CT]^C
1592 GGGCCC
BcnI 892 CCCGG CC^[GC]GG
1590 CCGGG
BfaI 203 CTAG C^TAG
1606 CTAG
BsiHKAI 194 GAGCTC G[AT]GC[AT]^C
1259 GTGCAC
BsmFI 501 GGGAC GGGAC(10/14)
1527 GGGAC
BstAPI 1360 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1369 GCAGCAGCTGC
DraII 726 AGGGCCT [GA]G^G[ACGT]CC[CT]
1592 GGGCCCT
EcoO109I 726 AGGGCCT [GA]G^G[ACGT]CC[CT]
1592 GGGCCCT
MaeI 203 CTAG C^TAG
1606 CTAG
MboII 309 TCTTC GAAGA(8/7)
861 TCTTC
MslI 313 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
648 CATTCCTGTG
MspA1I 1373 CAGCTG C[AC]G^C[GT]G
1454 CAGCTG
NciI 892 CCCGG CC^[GC]GG
1590 CCGGG
PstI 442 CTGCAG CTGCA^G
1367 CTGCAG
PvuII 1373 CAGCTG CAG^CTG
1454 CAGCTG
RsaI 1225 GTAC GT^AC
1615 GTAC
SfcI 442 CTGCAG C^T[GA][CT]AG
1367 CTGCAG
TaqI 571 TCGA T^CGA
1332 TCGA
TfiI 163 GATTC G^A[AT]TC
384 GAATC
TthHB8I 571 TCGA T^CGA
1332 TCGA
XcmI 75 CCATAGACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
644 CCAACATTCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoNI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp509I TspRI