Restriction map for
pF1KB7001
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BciVI BclI BfrI
BglI BsaAI BsiEI BssHII Bst1107I
BstZ17I Bsu36I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV FbaI FseI FspI HpaI
KasI KpnI KspI NarI NdeI
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SfiI SfoI SgrAI SmaI SnaBI
SpeI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2700 GACGTC GACGT^C
AccI 511 GTCTAC GT^[AC][GT]AC
AcyI 2700 GACGTC G[GA]^CG[CT]C
Alw44I 415 GTGCAC G^TGCAC
ApaI 2974 GGGCCC GGGCC^C
ApaLI 415 GTGCAC G^TGCAC
AvrII 2781 CCTAGG C^CTAGG
BalI 272 TGGCCA TGG^CCA
BbvCI 3080 CCTCAGC CCTCAGC(-5/-2)
BlnI 2781 CCTAGG C^CTAGG
BsaHI 2700 GACGTC G[GA]^CG[CT]C
BseRI 2707 GAGGAG GAGGAG(10/8)
BsiWI 3811 CGTACG C^GTACG
BspDI 2557 ATCGAT AT^CGAT
BspHI 3773 TCATGA T^CATGA
BspLU11I 3032 ACATGT A^CATGT
BsrFI 1804 GCCGGC [GA]^CCGG[CT]
BssSI 1282 CTCGTG CACGAG(-5/-1)
BstAPI 2894 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2356 GGTGACC G^GT[ACGT]ACC
BstPI 2356 GGTGACC G^GT[ACGT]ACC
Cfr10I 1804 GCCGGC [GA]^CCGG[CT]
ClaI 2557 ATCGAT AT^CGAT
DraIII 433 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2356 GGTGACC G^GT[ACGT]ACC
HaeII 1933 GGCGCT [GA]GCGC^[CT]
Hin1I 2700 GACGTC G[GA]^CG[CT]C
HincII 1114 GTCAAC GT[CT]^[GA]AC
HindII 1114 GTCAAC GT[CT]^[GA]AC
HindIII 3219 AAGCTT A^AGCTT
MfeI 2208 CAATTG C^AATTG
MluI 1305 ACGCGT A^CGCGT
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MunI 2208 CAATTG C^AATTG
NaeI 1804 GCCGGC GCC^GGC
NgoMIV 1804 GCCGGC G^CCGGC
RcaI 3773 TCATGA T^CATGA
SbfI 2664 CCTGCAGG CCTGCA^GG
ScaI 595 AGTACT AGT^ACT
SexAI 2092 ACCTGGT A^CC[AT]GGT
SmlI 2650 CTCAAG C^T[CT][GA]AG
SphI 632 GCATGC GCATG^C
Sse8387I 2664 CCTGCAGG CCTGCA^GG
SspI 3911 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 2476 TCCGGA T^CCGGA
3384 TCCGGA
AflIII 1305 ACGCGT A^C[GA][CT]GT
3032 ACATGT
AlwI 94 GATCC GGATC(4/5)
3655 GATCC
AlwNI 372 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
2895 CAGCATCTG
AvaI 1239 CTCGGG C^[CT]CG[GA]G
1922 CCCGAG
BglII 1049 AGATCT A^GATCT
1883 AGATCT
BlpI 750 GCTCAGC GC^T[ACGT]AGC
1254 GCTGAGC
Bpu1102I 750 GCTCAGC GC^T[ACGT]AGC
1254 GCTGAGC
BsaBI 2286 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3744 GATATTCATC
BsaI 677 GAGACC GGTCTC(1/5)
2656 GAGACC
BsaWI 2476 TCCGGA [AT]^CCGG[AT]
3384 TCCGGA
BseAI 2476 TCCGGA T^CCGGA
3384 TCCGGA
BsgI 1441 GTGCAG GTGCAG(16/14)
1897 GTGCAG
BsoBI 1239 CTCGGG C^[CT]CG[GA]G
1922 CCCGAG
Bsp1407I 569 TGTACA T^GTACA
1781 TGTACA
BspEI 2476 TCCGGA T^CCGGA
3384 TCCGGA
BsrBI 823 GAGCGG CCGCTC(-3/-3)
3676 GAGCGG
BsrDI 3120 CATTGC GCAATG(2/0)
1064 GCAATG
BsrGI 569 TGTACA T^GTACA
1781 TGTACA
BstBI 1187 TTCGAA TT^CGAA
3701 TTCGAA
CelII 750 GCTCAGC GC^T[ACGT]AGC
1254 GCTGAGC
EcoNI 2965 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3240 CCTAAACAAGG
EcoRI 932 GAATTC G^AATTC
2431 GAATTC
MamI 2286 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3744 GATATTCATC
MroI 2476 TCCGGA T^CCGGA
3384 TCCGGA
NspV 1187 TTCGAA TT^CGAA
3701 TTCGAA
SfuI 1187 TTCGAA TT^CGAA
3701 TTCGAA
SspBI 569 TGTACA T^GTACA
1781 TGTACA
XcmI 1426 CCAGACAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2086 CCATTTACCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI