Restriction map for
pF1KB7003
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI ApaI AscI AspEI AvaI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BseRI
BsgI BsiWI BsoBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoO65I
FseI FspI HgaI Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 63 GGTACC G^GTACC
AccI 2877 GTCTAC GT^[AC][GT]AC
AflIII 3341 ACATGT A^C[GA][CT]GT
Alw44I 92 GTGCAC G^TGCAC
Aor51HI 1066 AGCGCT AGC^GCT
ApaLI 92 GTGCAC G^TGCAC
Asp700I 1903 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 63 GGTACC G^GTACC
AspI 2124 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2667 TGGCCA TGG^CCA
BamHI 1879 GGATCC G^GATCC
BclI 1173 TGATCA T^GATCA
BcnI 3695 CCGGG CC^[GC]GG
BlpI 205 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 205 GCTAAGC GC^T[ACGT]AGC
BsaBI 2161 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1995 GAGACC GGTCTC(1/5)
BsiEI 3250 CGATCG CG[GA][CT]^CG
BsmBI 3835 CGTCTC CGTCTC(1/5)
Bsp1407I 1635 TGTACA T^GTACA
BspLU11I 3341 ACATGT A^CATGT
BsrBI 3150 GAGCGG CCGCTC(-3/-3)
BsrGI 1635 TGTACA T^GTACA
BstBI 3689 TTCGAA TT^CGAA
CelII 205 GCTAAGC GC^T[ACGT]AGC
CfoI 1067 GCGC GCG^C
DraIII 2100 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 710 CCATGG C^C[GA][CT]GG
EaeI 2667 TGGCCA [CT]^GGCC[GA]
Eco47III 1066 AGCGCT AGC^GCT
EcoRI 3804 GAATTC G^AATTC
EcoRV 701 GATATC GAT^ATC
FauI 1698 CCCGC CCCGC(4/6)
FbaI 1173 TGATCA T^GATCA
HaeII 1066 AGCGCT [GA]GCGC^[CT]
HapII 3695 CCGG C^CGG
HhaI 1067 GCGC GCG^C
HinP1I 1067 GCGC G^CGC
HincII 3630 GTTGAC GT[CT]^[GA]AC
HindII 3630 GTTGAC GT[CT]^[GA]AC
HindIII 160 AAGCTT A^AGCTT
HpaII 3695 CCGG C^CGG
KpnI 63 GGTACC GGTAC^C
MamI 2161 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2667 TGGCCA TGG^CCA
MscI 2667 TGGCCA TGG^CCA
MspI 3695 CCGG C^CGG
NciI 3695 CCGGG CC^[GC]GG
NcoI 710 CCATGG C^CATGG
NdeI 2071 CATATG CA^TATG
NheI 2386 GCTAGC G^CTAGC
NspV 3689 TTCGAA TT^CGAA
PflFI 2124 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 3677 CTGCAG CTGCA^G
PvuI 3250 CGATCG CGAT^CG
SexAI 40 ACCAGGT A^CC[AT]GGT
SfuI 3689 TTCGAA TT^CGAA
SspBI 1635 TGTACA T^GTACA
SwaI 3212 ATTTAAAT ATTT^AAAT
Tth111I 2124 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 530 CCAGGGAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1903 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AclI 1801 AACGTT AA^CGTT
2923 AACGTT
AflII 2995 CTTAAG C^TTAAG
3156 CTTAAG
AspHI 92 GTGCAC G[AT]GC[AT]^C
2376 GAGCAC
BciVI 1650 GTATCC GTATCC(6/5)
2608 GTATCC
BfrI 2995 CTTAAG C^TTAAG
3156 CTTAAG
BmyI 92 GTGCAC G[AGT]GC[ACT]^C
2376 GAGCAC
BsiHKAI 92 GTGCAC G[AT]GC[AT]^C
2376 GAGCAC
Bsp1286I 92 GTGCAC G[AGT]GC[ACT]^C
2376 GAGCAC
BspHI 1593 TCATGA T^CATGA
2901 TCATGA
BstXI 3074 CCAGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3164 CCAATGGTGTGG
BstYI 1256 AGATCC [GA]^GATC[CT]
1879 GGATCC
MflI 1256 AGATCC [GA]^GATC[CT]
1879 GGATCC
MspA1I 2671 CAGCTG C[AC]G^C[GT]G
3226 CAGCTG
Psp1406I 1801 AACGTT AA^CGTT
2923 AACGTT
PvuII 2671 CAGCTG CAG^CTG
3226 CAGCTG
RcaI 1593 TCATGA T^CATGA
2901 TCATGA
SphI 2108 GCATGC GCATG^C
3085 GCATGC
XhoII 1256 AGATCC [GA]^GATC[CT]
1879 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AvaII BanI BbsI BbvI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI EarI Eco81I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I