Restriction map for
pF1KB7009
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BbeI BbrPI BclI BfrI
BglI BlnI BsaBI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SfiI SfoI SfuI
SgrAI SnaBI SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 571 GAATTT [GA]^AATT[CT]
Alw44I 2154 GTGCAC G^TGCAC
Aor51HI 3092 AGCGCT AGC^GCT
ApaLI 2154 GTGCAC G^TGCAC
ApoI 571 GAATTT [GA]^AATT[CT]
BamHI 3132 GGATCC G^GATCC
BbsI 535 GAAGAC GAAGAC(2/6)
BciVI 3197 GTATCC GTATCC(6/5)
BglII 1520 AGATCT A^GATCT
BpuAI 535 GAAGAC GAAGAC(2/6)
BsaAI 3103 CACGTA [CT]AC^GT[GA]
BsaI 1778 GGTCTC GGTCTC(1/5)
BsmBI 3079 GAGACG CGTCTC(1/5)
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrDI 2581 GCAATG GCAATG(2/0)
BsrFI 2983 GCCGGC [GA]^CCGG[CT]
Cfr10I 2983 GCCGGC [GA]^CCGG[CT]
DrdI 2842 GACAATAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3092 AGCGCT AGC^GCT
HincII 840 GTCGAC GT[CT]^[GA]AC
HindII 840 GTCGAC GT[CT]^[GA]AC
HindIII 2951 AAGCTT A^AGCTT
NaeI 2983 GCCGGC GCC^GGC
NcoI 732 CCATGG C^CATGG
NdeI 1113 CATATG CA^TATG
NgoMIV 2983 GCCGGC G^CCGGC
NheI 129 GCTAGC G^CTAGC
NruI 2307 TCGCGA TCG^CGA
PleI 1227 GAGTC GAGTC(4/5)
SalI 840 GTCGAC G^TCGAC
SapI 3070 GAAGAGC GCTCTTC(1/4)
SbfI 783 CCTGCAGG CCTGCA^GG
SexAI 431 ACCAGGT A^CC[AT]GGT
SmaI 2882 CCCGGG CCC^GGG
SpeI 1303 ACTAGT A^CTAGT
SphI 2654 GCATGC GCATG^C
Sse8387I 783 CCTGCAGG CCTGCA^GG
Tsp509I 572 AATT ^AATT
XmaCI 2882 CCCGGG C^CCGGG
XmaI 2882 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
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Acc65I 790 GGTACC G^GTACC
3180 GGTACC
AccI 840 GTCGAC GT^[AC][GT]AC
1948 GTCTAC
AccII 40 CGCG CG^CG
2308 CGCG
AcyI 154 GACGCC G[GA]^CG[CT]C
1388 GACGCC
AocI 60 CCTGAGG CC^T[ACGT]AGG
1768 CCTGAGG
Asp718I 790 GGTACC G^GTACC
3180 GGTACC
BbvCI 265 CCTCAGC CCTCAGC(-5/-2)
818 CCTCAGC
BsaHI 154 GACGCC G[GA]^CG[CT]C
1388 GACGCC
BsaWI 1298 ACCGGA [AT]^CCGG[AT]
2126 ACCGGA
BsiEI 103 CGGCCG CG[GA][CT]^CG
246 CGGCCG
BsiWI 2630 CGTACG C^GTACG
3105 CGTACG
BstUI 40 CGCG CG^CG
2308 CGCG
Bsu36I 60 CCTGAGG CC^T[ACGT]AGG
1768 CCTGAGG
EagI 103 CGGCCG C^GGCCG
246 CGGCCG
EclXI 103 CGGCCG C^GGCCG
246 CGGCCG
Eco52I 103 CGGCCG C^GGCCG
246 CGGCCG
Eco81I 60 CCTGAGG CC^T[ACGT]AGG
1768 CCTGAGG
EcoNI 1191 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2988 CCTGTGCCAGG
HgaI 154 GACGC GACGC(5/10)
1388 GACGC
Hin1I 154 GACGCC G[GA]^CG[CT]C
1388 GACGCC
KpnI 790 GGTACC GGTAC^C
3180 GGTACC
MseI 299 TTAA T^TAA
2858 TTAA
MvnI 40 CGCG CG^CG
2308 CGCG
NspI 1326 ACATGC [GA]CATG^[CT]
2654 GCATGC
PaeR7I 1266 CTCGAG C^TCGAG
2780 CTCGAG
PflMI 3036 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3114 CCAGCCTTTGG
SacI 475 GAGCTC GAGCT^C
1744 GAGCTC
ScaI 456 AGTACT AGT^ACT
1538 AGTACT
SmlI 1266 CTCGAG C^T[CT][GA]AG
2780 CTCGAG
StuI 1029 AGGCCT AGG^CCT
1461 AGGCCT
TfiI 70 GATTC G^A[AT]TC
1159 GATTC
Tru9I 299 TTAA T^TAA
2858 TTAA
Van91I 3036 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3114 CCAGCCTTTGG
XhoI 1266 CTCGAG C^TCGAG
2780 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII