Restriction map for
pF1KB7016
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI AspI
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1828 GGTACC G^GTACC
AhdI 2341 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1822 CCTGAGG CC^T[ACGT]AGG
Asp700I 393 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1828 GGTACC G^GTACC
AspEI 2341 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1445 TGCGCA TGC^GCA
BamHI 1140 GGATCC G^GATCC
BbeI 163 GGCGCC GGCGC^C
BsaBI 888 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 905 TCATGA T^CATGA
BsrDI 806 CATTGC GCAATG(2/0)
BssSI 1457 CACGAG CACGAG(-5/-1)
BstAPI 1029 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2196 CCAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1822 CCTGAGG CC^T[ACGT]AGG
DraIII 1781 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2341 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 813 GAAGAG CTCTTC(1/4)
Eco81I 1822 CCTGAGG CC^T[ACGT]AGG
EcoNI 2022 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1445 TGCGCA TGC^GCA
HincII 2322 GTTAAC GT[CT]^[GA]AC
HindII 2322 GTTAAC GT[CT]^[GA]AC
HinfI 2176 GACTC G^A[ACGT]TC
HpaI 2322 GTTAAC GTT^AAC
KasI 163 GGCGCC G^GCGCC
KpnI 1828 GGTACC GGTAC^C
Ksp632I 813 GAAGAG CTCTTC(1/4)
MamI 888 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2323 TTAA T^TAA
NaeI 1959 GCCGGC GCC^GGC
NarI 163 GGCGCC GG^CGCC
NgoMIV 1959 GCCGGC G^CCGGC
PleI 2176 GACTC GAGTC(4/5)
RcaI 905 TCATGA T^CATGA
SapI 813 GAAGAGC GCTCTTC(1/4)
SfoI 163 GGCGCC GGC^GCC
TaqI 2375 TCGA T^CGA
Tru9I 2323 TTAA T^TAA
TthHB8I 2375 TCGA T^CGA
XmnI 393 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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Aor51HI 92 AGCGCT AGC^GCT
2366 AGCGCT
BbsI 1513 GTCTTC GAAGAC(2/6)
1522 GTCTTC
BcgI 2441 CGATGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
31 GCAGCGGCCTCG
BfaI 461 CTAG C^TAG
1784 CTAG
BpuAI 1513 GTCTTC GAAGAC(2/6)
1522 GTCTTC
BsaI 523 GAGACC GGTCTC(1/5)
1717 GAGACC
BsiEI 41 CGGCCG CG[GA][CT]^CG
110 CGGCCG
BsmBI 1669 GAGACG CGTCTC(1/5)
2377 GAGACG
BspMI 682 ACCTGC ACCTGC(4/8)
1955 ACCTGC
BsrFI 1591 ACCGGC [GA]^CCGG[CT]
1959 GCCGGC
Cfr10I 1591 ACCGGC [GA]^CCGG[CT]
1959 GCCGGC
EagI 41 CGGCCG C^GGCCG
110 CGGCCG
EclXI 41 CGGCCG C^GGCCG
110 CGGCCG
Eco47III 92 AGCGCT AGC^GCT
2366 AGCGCT
Eco52I 41 CGGCCG C^GGCCG
110 CGGCCG
MaeI 461 CTAG C^TAG
1784 CTAG
NcoI 1265 CCATGG C^CATGG
1325 CCATGG
NspI 798 ACATGC [GA]CATG^[CT]
2300 GCATGT
SbfI 1612 CCTGCAGG CCTGCA^GG
1965 CCTGCAGG
SmlI 1126 CTCAAG C^T[CT][GA]AG
1374 CTCAAG
Sse8387I 1612 CCTGCAGG CCTGCA^GG
1965 CCTGCAGG
StuI 1208 AGGCCT AGG^CCT
2393 AGGCCT
Tsp509I 394 AATT ^AATT
2312 AATT
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TseI
Tsp45I TspRI Van91I XcmI XhoII
XmaCI XmaI