Restriction map for
pF1KB9017
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfaI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NarI NdeI
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1073 GTAGAC GT^[AC][GT]AC
AflII 1230 CTTAAG C^TTAAG
AlwNI 742 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 345 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1144 GAGCTC G[GA]GC[CT]^C
BbsI 921 GAAGAC GAAGAC(2/6)
BbvI 56 GCTGC GCAGC(8/12)
BfrI 1230 CTTAAG C^TTAAG
BglI 1106 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 921 GAAGAC GAAGAC(2/6)
BsaBI 625 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 677 GAGACC GGTCTC(1/5)
BsrDI 23 GCAATG GCAATG(2/0)
BsrFI 1013 GCCGGC [GA]^CCGG[CT]
BstAPI 409 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1062 TTCGAA TT^CGAA
BstF5I 288 GGATG GGATG(2/0)
BstXI 1049 CCATTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1013 GCCGGC [GA]^CCGG[CT]
DraII 743 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 681 CCATGG C^C[GA][CT]GG
EaeI 494 TGGCCG [CT]^GGCC[GA]
EarI 934 GAAGAG CTCTTC(1/4)
EcoNI 46 CCTTGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 743 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1065 GAATTC G^AATTC
EcoRV 625 GATATC GAT^ATC
EcoT22I 818 ATGCAT ATGCA^T
Fnu4HI 56 GCTGC GC^[ACGT]GC
FokI 288 GGATG GGATG(9/13)
HaeII 938 AGCGCC [GA]GCGC^[CT]
ItaI 56 GCTGC GC^[ACGT]GC
Ksp632I 934 GAAGAG CTCTTC(1/4)
MaeIII 1214 GTAAC ^GT[ACGT]AC
MamI 625 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 219 CAGCTG C[AC]G^C[GT]G
NaeI 1013 GCCGGC GCC^GGC
NcoI 681 CCATGG C^CATGG
NgoMIV 1013 GCCGGC G^CCGGC
NsiI 818 ATGCAT ATGCA^T
NspV 1062 TTCGAA TT^CGAA
PflMI 816 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 219 CAGCTG CAG^CTG
SacI 1144 GAGCTC GAGCT^C
SapI 934 GAAGAGC GCTCTTC(1/4)
SfaNI 373 GCATC GCATC(5/9)
SfcI 443 CTACAG C^T[GA][CT]AG
SfuI 1062 TTCGAA TT^CGAA
TfiI 1030 GATTC G^A[AT]TC
TseI 56 GCTGC G^C[AT]GC
Van91I 816 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 345 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 1011 CCGC CCGC(-3/-1)
723 GCGG
AfaI 153 GTAC GT^AC
501 GTAC
Alw44I 306 GTGCAC G^TGCAC
1082 GTGCAC
AlwI 28 GATCC GGATC(4/5)
214 GATCC
ApaLI 306 GTGCAC G^TGCAC
1082 GTGCAC
AseI 687 ATTAAT AT^TAAT
711 ATTAAT
AsnI 687 ATTAAT AT^TAAT
711 ATTAAT
BciVI 1312 GGATAC GTATCC(6/5)
726 GTATCC
BcnI 497 CCGGG CC^[GC]GG
1280 CCCGG
BpmI 970 CTCCAG CTGGAG(16/14)
1172 CTGGAG
BseRI 60 CTCCTC GAGGAG(10/8)
322 GAGGAG
BsiYI 46 CCTTGCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
816 CCATGCATTGG
BslI 46 CCTTGCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
816 CCATGCATTGG
CfoI 939 GCGC GCG^C
1240 GCGC
HhaI 939 GCGC GCG^C
1240 GCGC
HinP1I 939 GCGC G^CGC
1240 GCGC
HphI 1158 GGTGA GGTGA(8/7)
912 TCACC
MslI 175 CATACTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1050 CATTCCAATG
NciI 497 CCGGG CC^[GC]GG
1280 CCCGG
NspI 503 ACATGC [GA]CATG^[CT]
600 ACATGC
PaeR7I 140 CTCGAG C^TCGAG
512 CTCGAG
PshBI 687 ATTAAT AT^TAAT
711 ATTAAT
RsaI 153 GTAC GT^AC
501 GTAC
XhoI 140 CTCGAG C^TCGAG
512 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI ApoI AspHI AvaI
AvaII BmyI BsaJI BsiHKAI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I HaeIII HapII HinfI
HpaII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SmlI StyI TaqI Tru9I Tsp509I
TspRI TthHB8I