Restriction map for
pF1KB9097
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AclI AflII ApaI AscI
AseI AsnI AspI BamHI BciVI
BfrI BlpI Bpu1102I BsaBI BsiWI
BsmBI BsmI BspHI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CpoI DrdI EcoO65I EcoRI EcoT22I
FseI HincII HindII HpaI MamI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 1532 GGTACC G^GTACC
AccIII 13 TCCGGA T^CCGGA
AflIII 652 ACGTGT A^C[GA][CT]GT
AgeI 1529 ACCGGT A^CCGGT
AhdI 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 669 GTGCAC G^TGCAC
Aor51HI 2941 AGCGCT AGC^GCT
ApaLI 669 GTGCAC G^TGCAC
Asp718I 1532 GGTACC G^GTACC
AspEI 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 669 GTGCAC G[AT]GC[AT]^C
AviII 3731 TGCGCA TGC^GCA
AvrII 1224 CCTAGG C^CTAGG
BbeI 1409 GGCGCC GGCGC^C
BbrPI 651 CACGTG CAC^GTG
BbvCI 855 CCTCAGC CCTCAGC(-5/-2)
BclI 2804 TGATCA T^GATCA
BglII 1799 AGATCT A^GATCT
BlnI 1224 CCTAGG C^CTAGG
BseAI 13 TCCGGA T^CCGGA
BsiEI 2273 CGGCCG CG[GA][CT]^CG
BsiHKAI 669 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1805 TGTACA T^GTACA
BspDI 2401 ATCGAT AT^CGAT
BspEI 13 TCCGGA T^CCGGA
BspMI 4093 ACCTGC ACCTGC(4/8)
BsrBI 4063 GAGCGG CCGCTC(-3/-3)
BsrDI 1107 CATTGC GCAATG(2/0)
BsrGI 1805 TGTACA T^GTACA
BssHII 719 GCGCGC G^CGCGC
BssSI 2422 CACGAG CACGAG(-5/-1)
BstAPI 953 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2401 ATCGAT AT^CGAT
DraI 2547 TTTAAA TTT^AAA
DraIII 3175 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2273 CGGCCG C^GGCCG
Eam1105I 3577 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2273 CGGCCG C^GGCCG
Eco47III 2941 AGCGCT AGC^GCT
Eco52I 2273 CGGCCG C^GGCCG
FbaI 2804 TGATCA T^GATCA
FspI 3731 TGCGCA TGC^GCA
HindIII 3146 AAGCTT A^AGCTT
KasI 1409 GGCGCC G^GCGCC
KpnI 1532 GGTACC GGTAC^C
KspI 318 CCGCGG CCGC^GG
MfeI 3168 CAATTG C^AATTG
MroI 13 TCCGGA T^CCGGA
MunI 3168 CAATTG C^AATTG
NarI 1409 GGCGCC GG^CGCC
PaeR7I 277 CTCGAG C^TCGAG
PflMI 1637 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1529 ACCGGT A^CCGGT
PmaCI 651 CACGTG CAC^GTG
PmlI 651 CACGTG CAC^GTG
SacII 318 CCGCGG CCGC^GG
SapI 2305 GAAGAGC GCTCTTC(1/4)
SfoI 1409 GGCGCC GGC^GCC
SmaI 2691 CCCGGG CCC^GGG
SspBI 1805 TGTACA T^GTACA
Van91I 1637 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1673 CCAACAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 277 CTCGAG C^TCGAG
XmaCI 2691 CCCGGG C^CCGGG
XmaI 2691 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1471 GACGTC GACGT^C
3577 GACGTC
AcsI 1373 AAATTT [GA]^AATT[CT]
2169 GAATTT
AocI 396 CCTGAGG CC^T[ACGT]AGG
1954 CCTCAGG
ApoI 1373 AAATTT [GA]^AATT[CT]
2169 GAATTT
Asp700I 327 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3159 GAAGGAGTTC
BalI 2234 TGGCCA TGG^CCA
3086 TGGCCA
BcgI 155 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3654 GCAAGTCCTTCG
BglI 920 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3613 GCCACACTGGC
BsaAI 651 CACGTG [CT]AC^GT[GA]
1159 TACGTG
BsgI 153 CTGCAC GTGCAG(16/14)
2176 CTGCAC
Bsu36I 396 CCTGAGG CC^T[ACGT]AGG
1954 CCTCAGG
Eco81I 396 CCTGAGG CC^T[ACGT]AGG
1954 CCTCAGG
EcoNI 967 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1224 CCTAGGAGAGG
EcoRV 2404 GATATC GAT^ATC
3472 GATATC
HgaI 3465 GACGC GACGC(5/10)
3902 GCGTC
MluNI 2234 TGGCCA TGG^CCA
3086 TGGCCA
MscI 2234 TGGCCA TGG^CCA
3086 TGGCCA
NcoI 554 CCATGG C^CATGG
1760 CCATGG
PshAI 538 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3700 GACAACTGTC
PstI 714 CTGCAG CTGCA^G
2442 CTGCAG
ScaI 248 AGTACT AGT^ACT
2933 AGTACT
SphI 908 GCATGC GCATG^C
3116 GCATGC
StuI 2527 AGGCCT AGG^CCT
3763 AGGCCT
XmnI 327 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3159 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII