Restriction map for
pF1KB9267
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstEII BstF5I
BstPI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DpnII
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HpaI HphI KasI
KpnI KspI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspI PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 42 CGCG CG^CG
AciI 41 CCGC CCGC(-3/-1)
AcsI 143 AAATTC [GA]^AATT[CT]
AfaI 243 GTAC GT^AC
ApoI 143 AAATTC [GA]^AATT[CT]
AvaI 373 CTCGAG C^[CT]CG[GA]G
BbvCI 51 CCTCAGC CCTCAGC(-5/-2)
BbvI 338 GCTGC GCAGC(8/12)
BcnI 182 CCCGG CC^[GC]GG
BfaI 358 CTAG C^TAG
BmyI 164 GTGCCC G[AGT]GC[ACT]^C
BpmI 247 CTGGAG CTGGAG(16/14)
BsaJI 8 CCAAGG C^C[ACGT][ACGT]GG
BsoBI 373 CTCGAG C^[CT]CG[GA]G
Bsp1286I 164 GTGCCC G[AGT]GC[ACT]^C
BssSI 31 CTCGTG CACGAG(-5/-1)
BstBI 103 TTCGAA TT^CGAA
BstNI 246 CCTGG CC^[AT]GG
BstUI 42 CGCG CG^CG
BstXI 8 CCAAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 258 TTTAAA TTT^AAA
EarI 328 GAAGAG CTCTTC(1/4)
EcoRII 246 CCTGG ^CC[AT]GG
EcoT14I 8 CCAAGG C^C[AT][AT]GG
Fnu4HI 338 GCTGC GC^[ACGT]GC
HaeII 403 GGCGCT [GA]GCGC^[CT]
HapII 183 CCGG C^CGG
HindIII 254 AAGCTT A^AGCTT
HpaII 183 CCGG C^CGG
ItaI 338 GCTGC GC^[ACGT]GC
Ksp632I 328 GAAGAG CTCTTC(1/4)
MaeI 358 CTAG C^TAG
MaeII 128 ACGT A^CGT
MseI 259 TTAA T^TAA
MspI 183 CCGG C^CGG
MvaI 246 CCTGG CC^[AT]GG
MvnI 42 CGCG CG^CG
NciI 182 CCCGG CC^[GC]GG
NspV 103 TTCGAA TT^CGAA
PaeR7I 373 CTCGAG C^TCGAG
RsaI 243 GTAC GT^AC
SfcI 81 CTACAG C^T[GA][CT]AG
SfuI 103 TTCGAA TT^CGAA
SmlI 373 CTCGAG C^T[CT][GA]AG
SpeI 357 ACTAGT A^CTAGT
StyI 8 CCAAGG C^C[AT][AT]GG
Tru9I 259 TTAA T^TAA
TseI 338 GCTGC G^C[AT]GC
Tsp509I 144 AATT ^AATT
XhoI 373 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 27 GGTCC G^G[AT]CC
381 GGACC
BsiYI 342 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCTCGAGATGG
BslI 342 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
372 CCTCGAGATGG
BssKI 182 CCCGG ^CC[ACGT]GG
246 CCTGG
Cfr13I 27 GGTCC G^G[ACGT]CC
381 GGACC
DdeI 52 CTCAG C^T[ACGT]AG
76 CTGAG
HinfI 98 GATTC G^A[ACGT]TC
234 GATTC
NlaIII 92 CATG CATG^
112 CATG
Sau96I 27 GGTCC G^G[ACGT]CC
381 GGACC
ScrFI 182 CCCGG CC^[ACGT]GG
246 CCTGG
SfaNI 86 GATGC GCATC(5/9)
135 GCATC
TaqI 104 TCGA T^CGA
374 TCGA
TfiI 98 GATTC G^A[AT]TC
234 GATTC
TthHB8I 104 TCGA T^CGA
374 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI CfoI HhaI HinP1I MboII
MnlI MwoI