Restriction map for
pF1KB9397
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BbeI BbrPI BbvCI BcgI BfrI
BsaAI BseRI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tsp45I
Tth111I Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 1733 TCCGGA T^CCGGA
AflIII 1163 ACGTGT A^C[GA][CT]GT
AlwNI 185 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1848 GAGCAC G[AT]GC[AT]^C
AvrII 1858 CCTAGG C^CTAGG
BamHI 1730 GGATCC G^GATCC
BanI 550 GGTGCC G^G[CT][GA]CC
BanII 763 GGGCTC G[GA]GC[CT]^C
BciVI 686 GTATCC GTATCC(6/5)
BclI 587 TGATCA T^GATCA
BglI 264 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1858 CCTAGG C^CTAGG
BlpI 1708 GCTGAGC GC^T[ACGT]AGC
BpmI 1786 CTCCAG CTGGAG(16/14)
Bpu1102I 1708 GCTGAGC GC^T[ACGT]AGC
BsaBI 1039 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 175 GAGACC GGTCTC(1/5)
BsaWI 1733 TCCGGA [AT]^CCGG[AT]
BseAI 1733 TCCGGA T^CCGGA
BsgI 812 CTGCAC GTGCAG(16/14)
BsiHKAI 1848 GAGCAC G[AT]GC[AT]^C
BsmBI 1865 GAGACG CGTCTC(1/5)
BsmFI 104 GGGAC GGGAC(10/14)
BsmI 149 GCATTC GAATGC(1/-1)
BspEI 1733 TCCGGA T^CCGGA
BsrBI 1759 GAGCGG CCGCTC(-3/-3)
BssSI 1585 CACGAG CACGAG(-5/-1)
BstAPI 1674 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1708 GCTGAGC GC^T[ACGT]AGC
DraII 186 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1275 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 454 GACAGTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1825 CCATGG C^C[GA][CT]GG
EcoNI 1853 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 186 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 612 GCGGG CCCGC(4/6)
FbaI 587 TGATCA T^GATCA
HincII 1155 GTTAAC GT[CT]^[GA]AC
HindII 1155 GTTAAC GT[CT]^[GA]AC
HindIII 1117 AAGCTT A^AGCTT
HpaI 1155 GTTAAC GTT^AAC
MamI 1039 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1733 TCCGGA T^CCGGA
MspA1I 610 CAGCGG C[AC]G^C[GT]G
NcoI 1825 CCATGG C^CATGG
NdeI 856 CATATG CA^TATG
NspI 46 GCATGT [GA]CATG^[CT]
PpuMI 186 AGGACCT [GA]G^G[AT]CC[CT]
PstI 556 CTGCAG CTGCA^G
SbfI 555 CCTGCAGG CCTGCA^GG
ScaI 643 AGTACT AGT^ACT
SmaI 821 CCCGGG CCC^GGG
Sse8387I 555 CCTGCAGG CCTGCA^GG
XbaI 384 TCTAGA T^CTAGA
XmaCI 821 CCCGGG C^CCGGG
XmaI 821 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 901 GAATTT [GA]^AATT[CT]
1218 AAATTT
AcyI 3 GGCGTC G[GA]^CG[CT]C
936 GGCGTC
ApoI 901 GAATTT [GA]^AATT[CT]
1218 AAATTT
Asp700I 800 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAACAGTTTC
AvaI 821 CCCGGG C^[CT]CG[GA]G
1106 CCCGAG
BalI 718 TGGCCA TGG^CCA
1772 TGGCCA
BglII 802 AGATCT A^GATCT
1454 AGATCT
BsaHI 3 GGCGTC G[GA]^CG[CT]C
936 GGCGTC
BsoBI 821 CCCGGG C^[CT]CG[GA]G
1106 CCCGAG
BspMI 189 ACCTGC ACCTGC(4/8)
810 ACCTGC
BsrDI 83 CATTGC GCAATG(2/0)
1447 GCAATG
EaeI 718 TGGCCA [CT]^GGCC[GA]
1772 TGGCCA
EcoT14I 1825 CCATGG C^C[AT][AT]GG
1858 CCTAGG
HgaI 4 GCGTC GACGC(5/10)
937 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
936 GGCGTC
MaeIII 1842 GTTAC ^GT[ACGT]AC
1870 GTAAC
MluNI 718 TGGCCA TGG^CCA
1772 TGGCCA
MscI 718 TGGCCA TGG^CCA
1772 TGGCCA
MslI 544 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1138 CACCATGATG
StuI 1088 AGGCCT AGG^CCT
1538 AGGCCT
StyI 1825 CCATGG C^C[AT][AT]GG
1858 CCTAGG
TaqI 252 TCGA T^CGA
783 TCGA
TthHB8I 252 TCGA T^CGA
783 TCGA
XmnI 800 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp509I TspRI XhoII