Restriction map for
pF1KSDA0112
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MaeII
MamI MboI MfeI MflI MluI
MroI MseI MunI NarI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsaI SacI SalI SapI
Sau3AI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 1026 GAATTC [GA]^AATT[CT]
AcyI 120 GGCGTC G[GA]^CG[CT]C
Alw44I 76 GTGCAC G^TGCAC
AlwNI 184 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 975 GGGCCC GGGCC^C
ApaLI 76 GTGCAC G^TGCAC
ApoI 1026 GAATTC [GA]^AATT[CT]
AspHI 76 GTGCAC G[AT]GC[AT]^C
BbsI 381 GAAGAC GAAGAC(2/6)
BbvCI 838 CCTCAGC CCTCAGC(-5/-2)
BcgI 140 CGACCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 854 CTAG C^TAG
BpmI 88 CTGGAG CTGGAG(16/14)
BpuAI 381 GAAGAC GAAGAC(2/6)
BsaHI 120 GGCGTC G[GA]^CG[CT]C
BsaI 678 GGTCTC GGTCTC(1/5)
BsiHKAI 76 GTGCAC G[AT]GC[AT]^C
BsmAI 679 GTCTC GTCTC(1/5)
BspHI 821 TCATGA T^CATGA
BspMI 113 ACCTGC ACCTGC(4/8)
BsrDI 485 GCAATG GCAATG(2/0)
BstAPI 588 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 662 CCATGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 125 CGGACCG CG^G[AT]CCG
DdeI 839 CTCAG C^T[ACGT]AG
EagI 611 CGGCCG C^GGCCG
EclXI 611 CGGCCG C^GGCCG
Eco52I 611 CGGCCG C^GGCCG
EcoNI 714 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1026 GAATTC G^AATTC
HgaI 121 GCGTC GACGC(5/10)
Hin1I 120 GGCGTC G[GA]^CG[CT]C
HinfI 522 GATTC G^A[ACGT]TC
HphI 405 GGTGA GGTGA(8/7)
KspI 322 CCGCGG CCGC^GG
MaeI 854 CTAG C^TAG
PstI 94 CTGCAG CTGCA^G
RcaI 821 TCATGA T^CATGA
RsrII 125 CGGACCG CG^G[AT]CCG
SacII 322 CCGCGG CCGC^GG
SexAI 389 ACCTGGT A^CC[AT]GGT
SmlI 358 CTCAAG C^T[CT][GA]AG
TfiI 522 GATTC G^A[AT]TC
Tsp45I 850 GTGAC ^GT[GC]AC
Tsp509I 1027 AATT ^AATT
TspRI 415 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 446 CCCGGG C^[CT]CG[GA]G
734 CCCGGG
BanI 477 GGTGCC G^G[CT][GA]CC
729 GGTGCC
BglI 356 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
920 GCCAGAAGGGC
BsaWI 128 ACCGGA [AT]^CCGG[AT]
628 ACCGGA
BsoBI 446 CCCGGG C^[CT]CG[GA]G
734 CCCGGG
BstF5I 366 CATCC GGATG(2/0)
846 GGATG
DsaI 239 CCACGG C^C[GA][CT]GG
322 CCGCGG
EarI 253 GAAGAG CTCTTC(1/4)
996 GAAGAG
FokI 366 CATCC GGATG(9/13)
846 GGATG
Ksp632I 253 GAAGAG CTCTTC(1/4)
996 GAAGAG
MaeIII 572 GTAAC ^GT[ACGT]AC
850 GTGAC
MslI 69 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CATGCAAGTG
NaeI 613 GCCGGC GCC^GGC
944 GCCGGC
NgoMIV 613 GCCGGC G^CCGGC
944 GCCGGC
NlaIII 663 CATG CATG^
822 CATG
PflMI 9 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
458 CCAAAGAATGG
SfcI 94 CTGCAG C^T[GA][CT]AG
181 CTACAG
SmaI 446 CCCGGG CCC^GGG
734 CCCGGG
Van91I 9 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
458 CCAAAGAATGG
XmaCI 446 CCCGGG C^CCGGG
734 CCCGGG
XmaI 446 CCCGGG C^CCGGG
734 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AvaII BalI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV PvuII Sau96I ScrFI SfaNI
StyI TseI XcmI