Restriction map for
pF1KSDA0216
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI ApoI AscI Asp700I Asp718I
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BsaBI BsiWI
BspDI BspHI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EcoRI EcoRV EcoT22I FbaI HpaI
KpnI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
ScaI SfuI SgrAI SnaBI SpeI
SspI SwaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 3767 TCCGGA T^CCGGA
AhdI 4939 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4939 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5941 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3314 TGCGCA TGC^GCA
BbeI 1760 GGCGCC GGCGC^C
BcgI 2627 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1305 CACGTA [CT]AC^GT[GA]
BsaI 3230 GAGACC GGTCTC(1/5)
BsaWI 3767 TCCGGA [AT]^CCGG[AT]
BseAI 3767 TCCGGA T^CCGGA
BspEI 3767 TCCGGA T^CCGGA
BspLU11I 1379 ACATGT A^CATGT
BssSI 2470 CACGAG CACGAG(-5/-1)
BstEII 3331 GGTTACC G^GT[ACGT]ACC
BstPI 3331 GGTTACC G^GT[ACGT]ACC
EagI 2267 CGGCCG C^GGCCG
Eam1105I 4939 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2267 CGGCCG C^GGCCG
Eco52I 2267 CGGCCG C^GGCCG
EcoO65I 3331 GGTTACC G^GT[ACGT]ACC
FseI 2268 GGCCGGCC GGCCGG^CC
FspI 3314 TGCGCA TGC^GCA
HindIII 3712 AAGCTT A^AGCTT
KasI 1760 GGCGCC G^GCGCC
MroI 3767 TCCGGA T^CCGGA
NaeI 2269 GCCGGC GCC^GGC
NarI 1760 GGCGCC GG^CGCC
NgoMIV 2269 GCCGGC G^CCGGC
PaeR7I 3808 CTCGAG C^TCGAG
PflFI 5941 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 4922 ACCAGGT A^CC[AT]GGT
SfiI 730 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1760 GGCGCC GGC^GCC
SphI 3011 GCATGC GCATG^C
TfiI 163 GAATC G^A[AT]TC
Tsp509I 5270 AATT ^AATT
Tth111I 5941 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3808 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 607 GTCGAC GT^[AC][GT]AC
2362 GTCGAC
AflIII 1304 ACACGT A^C[GA][CT]GT
1379 ACATGT
Alw44I 2240 GTGCAC G^TGCAC
3130 GTGCAC
ApaLI 2240 GTGCAC G^TGCAC
3130 GTGCAC
AseI 835 ATTAAT AT^TAAT
1645 ATTAAT
AsnI 835 ATTAAT AT^TAAT
1645 ATTAAT
BsiEI 1212 CGACCG CG[GA][CT]^CG
2267 CGGCCG
BsmI 2868 GAATGC GAATGC(1/-1)
5773 GCATTC
Bsp1407I 1604 TGTACA T^GTACA
3660 TGTACA
BsrGI 1604 TGTACA T^GTACA
3660 TGTACA
DraIII 844 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
3343 CACATGGTG
KspI 2406 CCGCGG CCGC^GG
3288 CCGCGG
NcoI 2796 CCATGG C^CATGG
5429 CCATGG
NheI 1291 GCTAGC G^CTAGC
5533 GCTAGC
PshBI 835 ATTAAT AT^TAAT
1645 ATTAAT
SacII 2406 CCGCGG CCGC^GG
3288 CCGCGG
SalI 607 GTCGAC G^TCGAC
2362 GTCGAC
SmaI 2370 CCCGGG CCC^GGG
5309 CCCGGG
SspBI 1604 TGTACA T^GTACA
3660 TGTACA
XmaCI 2370 CCCGGG C^CCGGG
5309 CCCGGG
XmaI 2370 CCCGGG C^CCGGG
5309 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XbaI XcmI XhoII