Restriction map for
pF1KSDA0263
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AlwI ApaI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
ClaI CpoI DraI EagI EarI
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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AgeI 368 ACCGGT A^CCGGT
AhdI 1051 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 640 GTGCAC G^TGCAC
AocI 1096 CCTGAGG CC^T[ACGT]AGG
Aor51HI 167 AGCGCT AGC^GCT
ApaLI 640 GTGCAC G^TGCAC
Asp700I 198 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1051 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI 499 CTAG C^TAG
BlpI 573 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 573 GCTGAGC GC^T[ACGT]AGC
BsaAI 1264 TACGTG [CT]AC^GT[GA]
BsaWI 368 ACCGGT [AT]^CCGG[AT]
BsmBI 1069 CGTCTC CGTCTC(1/5)
BsmI 42 GAATGC GAATGC(1/-1)
BsrBI 967 GAGCGG CCGCTC(-3/-3)
BsrDI 869 GCAATG GCAATG(2/0)
BsrI 995 CCAGT ACTGG(1/-1)
BstAPI 1022 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 17 CCATGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1096 CCTGAGG CC^T[ACGT]AGG
CelII 573 GCTGAGC GC^T[ACGT]AGC
DrdI 61 GACCGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 840 TGGCCG [CT]^GGCC[GA]
Eam1105I 1051 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 167 AGCGCT AGC^GCT
Eco81I 1096 CCTGAGG CC^T[ACGT]AGG
HaeII 167 AGCGCT [GA]GCGC^[CT]
HphI 206 TCACC GGTGA(8/7)
MaeI 499 CTAG C^TAG
MluI 704 ACGCGT A^CGCGT
NcoI 17 CCATGG C^CATGG
PinAI 368 ACCGGT A^CCGGT
PstI 822 CTGCAG CTGCA^G
SacI 432 GAGCTC GAGCT^C
ScaI 833 AGTACT AGT^ACT
SfcI 822 CTGCAG C^T[GA][CT]AG
SmaI 158 CCCGGG CCC^GGG
StuI 974 AGGCCT AGG^CCT
XcmI 492 CCAGATGCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 158 CCCGGG C^CCGGG
XmaI 158 CCCGGG C^CCGGG
XmnI 198 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 686 CGCG CG^CG
705 CGCG
AcyI 769 GGCGTC G[GA]^CG[CT]C
1104 GGCGTC
AflIII 704 ACGCGT A^C[GA][CT]GT
1265 ACGTGT
AlwNI 568 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
774 CAGGGTCTG
AvaI 158 CCCGGG C^[CT]CG[GA]G
1147 CCCGAG
BsaHI 769 GGCGTC G[GA]^CG[CT]C
1104 GGCGTC
BsaI 59 GAGACC GGTCTC(1/5)
421 GAGACC
BseRI 1161 CTCCTC GAGGAG(10/8)
397 GAGGAG
BsoBI 158 CCCGGG C^[CT]CG[GA]G
1147 CCCGAG
BspMI 905 ACCTGC ACCTGC(4/8)
789 GCAGGT
BsrFI 368 ACCGGT [GA]^CCGG[CT]
1055 GCCGGT
BstUI 686 CGCG CG^CG
705 CGCG
Cfr10I 368 ACCGGT [GA]^CCGG[CT]
1055 GCCGGT
DpnII 130 GATC ^GATC
531 GATC
DraIII 133 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
135 CACTGTGTG
DsaI 17 CCATGG C^C[GA][CT]GG
843 CCGTGG
Hin1I 769 GGCGTC G[GA]^CG[CT]C
1104 GGCGTC
MboI 130 GATC ^GATC
531 GATC
MboII 528 GAAGA GAAGA(8/7)
675 GAAGA
MvnI 686 CGCG CG^CG
705 CGCG
NdeII 130 GATC ^GATC
531 GATC
NspI 581 GCATGC [GA]CATG^[CT]
785 GCATGC
PpuMI 482 AGGTCCC [GA]G^G[AT]CC[CT]
1155 GGGTCCC
Sau3AI 130 GATC ^GATC
531 GATC
SphI 581 GCATGC GCATG^C
785 GCATGC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI SfaNI SmlI StyI TseI
Tsp45I TspRI