##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BmyI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CfoI        Cfr10I      CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MroI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

BalI         1808  TGGCCA               TGG^CCA
BciVI         387  GGATAC               GTATCC(6/5)
BlpI         1745  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1745  GCTTAGC              GC^T[ACGT]AGC
BsaI         1852  GAGACC               GGTCTC(1/5)
BsmI         1288  GAATGC               GAATGC(1/-1)
BspDI        1325  ATCGAT               AT^CGAT
BsrDI          31  GCAATG               GCAATG(2/0)
CelII        1745  GCTTAGC              GC^T[ACGT]AGC
ClaI         1325  ATCGAT               AT^CGAT
DraI          133  TTTAAA               TTT^AAA
DsaI          915  CCATGG               C^C[GA][CT]GG
EaeI         1808  TGGCCA               [CT]^GGCC[GA]
FauI         1204  GCGGG                CCCGC(4/6)
HindIII       162  AAGCTT               A^AGCTT
MluNI        1808  TGGCCA               TGG^CCA
MscI         1808  TGGCCA               TGG^CCA
MspA1I       1202  CAGCGG               C[AC]G^C[GT]G
NcoI          915  CCATGG               C^CATGG
NdeI         1700  CATATG               CA^TATG
PflMI        1752  CCAGGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
TaqI         1326  TCGA                 T^CGA
TthHB8I      1326  TCGA                 T^CGA
Van91I       1752  CCAGGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AocI          227  CCTCAGG              CC^T[ACGT]AGG
              392  CCTTAGG             
BamHI        1128  GGATCC               G^GATCC
             1707  GGATCC              
BpmI         1617  CTCCAG               CTGGAG(16/14)
             1606  CTGGAG              
BspMI        1554  ACCTGC               ACCTGC(4/8)
             2037  ACCTGC              
BstXI          39  CCAAGAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              236  CCAGTCCTTTGG        
BstYI        1128  GGATCC               [GA]^GATC[CT]
             1707  GGATCC              
Bsu36I        227  CCTCAGG              CC^T[ACGT]AGG
              392  CCTTAGG             
Cac8I          24  GCCAGC               GC[ACGT]^[ACGT]GC
             1575  GCCAGC              
Eco81I        227  CCTCAGG              CC^T[ACGT]AGG
              392  CCTTAGG             
MflI         1128  GGATCC               [GA]^GATC[CT]
             1707  GGATCC              
MslI          118  CATGCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              367  CATCACAGTG          
NspI          117  ACATGC               [GA]CATG^[CT]
             2008  ACATGC              
PstI          551  CTGCAG               CTGCA^G
             2057  CTGCAG              
SfcI          551  CTGCAG               C^T[GA][CT]AG
             2057  CTGCAG              
Tsp45I          3  GTCAC                ^GT[GC]AC
             1955  GTCAC               
XhoII        1128  GGATCC               [GA]^GATC[CT]
             1707  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BfaI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI