Restriction map for
pF1KSDA0661
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbeI
BbvCI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI HphI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 2779 GTCTAC GT^[AC][GT]AC
AflIII 394 ACATGT A^C[GA][CT]GT
Asp700I 316 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1863 CCTAGG C^CTAGG
BbrPI 2635 CACGTG CAC^GTG
BcgI 2153 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2599 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1863 CCTAGG C^CTAGG
BsaAI 2635 CACGTG [CT]AC^GT[GA]
BsmBI 1150 GAGACG CGTCTC(1/5)
BspLU11I 394 ACATGT A^CATGT
BstAPI 502 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2657 AGATCC [GA]^GATC[CT]
DraIII 503 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 128 CCACGG C^C[GA][CT]GG
HincII 2835 GTTGAC GT[CT]^[GA]AC
HindII 2835 GTTGAC GT[CT]^[GA]AC
HindIII 1519 AAGCTT A^AGCTT
MflI 2657 AGATCC [GA]^GATC[CT]
MslI 1722 CACTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 2635 CACGTG CAC^GTG
PmlI 2635 CACGTG CAC^GTG
ScaI 770 AGTACT AGT^ACT
SgrAI 27 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1802 CCCGGG CCC^GGG
XhoII 2657 AGATCC [GA]^GATC[CT]
XmaCI 1802 CCCGGG C^CCGGG
XmaI 1802 CCCGGG C^CCGGG
XmnI 316 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 1498 GACGCC G[GA]^CG[CT]C
1615 GGCGTC
AviII 1385 TGCGCA TGC^GCA
2552 TGCGCA
BfaI 1225 CTAG C^TAG
1864 CTAG
BsaHI 1498 GACGCC G[GA]^CG[CT]C
1615 GGCGTC
BsmI 1005 GAATGC GAATGC(1/-1)
2186 GCATTC
Bsp1407I 638 TGTACA T^GTACA
2024 TGTACA
BsrGI 638 TGTACA T^GTACA
2024 TGTACA
BssSI 578 CTCGTG CACGAG(-5/-1)
2690 CTCGTG
BstXI 809 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1560 CCAGGCCAGTGG
EarI 123 CTCTTC CTCTTC(1/4)
1756 GAAGAG
FspI 1385 TGCGCA TGC^GCA
2552 TGCGCA
Hin1I 1498 GACGCC G[GA]^CG[CT]C
1615 GGCGTC
Ksp632I 123 CTCTTC CTCTTC(1/4)
1756 GAAGAG
MaeI 1225 CTAG C^TAG
1864 CTAG
MaeII 2636 ACGT A^CGT
2894 ACGT
MseI 920 TTAA T^TAA
2360 TTAA
NaeI 28 GCCGGC GCC^GGC
480 GCCGGC
NgoMIV 28 GCCGGC G^CCGGC
480 GCCGGC
SphI 983 GCATGC GCATG^C
1166 GCATGC
SspBI 638 TGTACA T^GTACA
2024 TGTACA
StuI 590 AGGCCT AGG^CCT
905 AGGCCT
Tru9I 920 TTAA T^TAA
2360 TTAA
Tsp45I 793 GTGAC ^GT[GC]AC
2278 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I Van91I XcmI