Restriction map for
pF1KSDA0704
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# NO CUT ENZYMES ( 107 enzymes) #
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Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbrPI BbvCI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI HindIII
KpnI KspI MluI MroI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 730 GACGTC GACGT^C
AccII 1716 CGCG CG^CG
AfaI 1692 GTAC GT^AC
AflII 2079 CTTAAG C^TTAAG
AlwNI 712 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1593 CCTGAGG CC^T[ACGT]AGG
AvaI 1560 CCCGAG C^[CT]CG[GA]G
BalI 1628 TGGCCA TGG^CCA
BamHI 2385 GGATCC G^GATCC
BbeI 1551 GGCGCC GGCGC^C
BbsI 642 GTCTTC GAAGAC(2/6)
BcnI 1529 CCCGG CC^[GC]GG
BfrI 2079 CTTAAG C^TTAAG
BpuAI 642 GTCTTC GAAGAC(2/6)
BsaBI 2115 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1818 TCCGGT [AT]^CCGG[AT]
BsoBI 1560 CCCGAG C^[CT]CG[GA]G
BsrDI 590 GCAATG GCAATG(2/0)
BssSI 620 CACGAG CACGAG(-5/-1)
BstAPI 1756 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1716 CGCG CG^CG
Bsu36I 1593 CCTGAGG CC^T[ACGT]AGG
DsaI 1982 CCATGG C^C[GA][CT]GG
EaeI 1628 TGGCCA [CT]^GGCC[GA]
Eco81I 1593 CCTGAGG CC^T[ACGT]AGG
HaeII 1551 GGCGCC [GA]GCGC^[CT]
HpaI 475 GTTAAC GTT^AAC
KasI 1551 GGCGCC G^GCGCC
MamI 2115 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1997 CAATTG C^AATTG
MluNI 1628 TGGCCA TGG^CCA
MscI 1628 TGGCCA TGG^CCA
MunI 1997 CAATTG C^AATTG
MvnI 1716 CGCG CG^CG
NaeI 1548 GCCGGC GCC^GGC
NarI 1551 GGCGCC GG^CGCC
NciI 1529 CCCGG CC^[GC]GG
NcoI 1982 CCATGG C^CATGG
NgoMIV 1548 GCCGGC G^CCGGC
RsaI 1692 GTAC GT^AC
SalI 89 GTCGAC G^TCGAC
SexAI 695 ACCTGGT A^CC[AT]GGT
SfoI 1551 GGCGCC GGC^GCC
SmlI 2079 CTTAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 89 GTCGAC GT^[AC][GT]AC
1319 GTAGAC
AcyI 730 GACGTC G[GA]^CG[CT]C
1551 GGCGCC
Asp700I 1840 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1987 GAAATTGTTC
BsaHI 730 GACGTC G[GA]^CG[CT]C
1551 GGCGCC
BspMI 2129 ACCTGC ACCTGC(4/8)
867 GCAGGT
BsrFI 1548 GCCGGC [GA]^CCGG[CT]
1635 GCCGGT
Cfr10I 1548 GCCGGC [GA]^CCGG[CT]
1635 GCCGGT
DrdI 1414 GACTCATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2644 GACCATCCTGTC
EcoNI 1617 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1704 CCTGGACAAGG
EcoT22I 339 ATGCAT ATGCA^T
1778 ATGCAT
Hin1I 730 GACGTC G[GA]^CG[CT]C
1551 GGCGCC
MaeII 731 ACGT A^CGT
1540 ACGT
NdeI 1418 CATATG CA^TATG
1835 CATATG
NsiI 339 ATGCAT ATGCA^T
1778 ATGCAT
PflMI 33 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2023 CCAGTTTTTGG
PpuMI 869 AGGTCCC [GA]G^G[AT]CC[CT]
1613 AGGACCT
PvuII 99 CAGCTG CAG^CTG
1087 CAGCTG
SacI 1289 GAGCTC GAGCT^C
1699 GAGCTC
SapI 71 GCTCTTC GCTCTTC(1/4)
1385 GCTCTTC
Van91I 33 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2023 CCAGTTTTTGG
XbaI 1924 TCTAGA T^CTAGA
2448 TCTAGA
XmnI 1840 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1987 GAAATTGTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanI BanII BbvI
BcgI BfaI BglII BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII