Restriction map for
pF1KSDA0814
##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp718I AspI
BciVI BclI BfrI BglII BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I Bsu36I ClaI DraI EagI
EclXI Eco52I Eco81I EcoNI EcoRI
FbaI FseI HindIII HpaI KpnI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI SacI
SalI ScaI SfuI SgrAI SnaBI
SpeI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AhdI 4352 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 534 AGCGCT AGC^GCT
Asp700I 450 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4352 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3926 TGCGCA TGC^GCA
AvrII 2062 CCTAGG C^CTAGG
BamHI 440 GGATCC G^GATCC
BlnI 2062 CCTAGG C^CTAGG
BsaBI 2094 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1780 GAGACC GGTCTC(1/5)
Bsp1407I 3680 TGTACA T^GTACA
BsrBI 4029 CCGCTC CCGCTC(-3/-3)
BsrGI 3680 TGTACA T^GTACA
BssHII 36 GCGCGC G^CGCGC
BssSI 1507 CTCGTG CACGAG(-5/-1)
BstEII 591 GGTCACC G^GT[ACGT]ACC
BstPI 591 GGTCACC G^GT[ACGT]ACC
CpoI 3884 CGGACCG CG^G[AT]CCG
DrdI 280 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4352 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 534 AGCGCT AGC^GCT
EcoO65I 591 GGTCACC G^GT[ACGT]ACC
EcoRV 1026 GATATC GAT^ATC
FspI 3926 TGCGCA TGC^GCA
HincII 3478 GTCAAC GT[CT]^[GA]AC
HindII 3478 GTCAAC GT[CT]^[GA]AC
KspI 459 CCGCGG CCGC^GG
MamI 2094 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RsrII 3884 CGGACCG CG^G[AT]CCG
SacII 459 CCGCGG CCGC^GG
SapI 1263 GCTCTTC GCTCTTC(1/4)
SexAI 284 ACCAGGT A^CC[AT]GGT
SfiI 1807 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3680 TGTACA T^GTACA
XmnI 450 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
307 GGCGCC
BbeI 25 GGCGCC GGCGC^C
307 GGCGCC
BbrPI 852 CACGTG CAC^GTG
3634 CACGTG
BbvCI 1833 GCTGAGG CCTCAGC(-5/-2)
2430 GCTGAGG
BcgI 3015 CGAAAACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
860 GCAATAACATCG
BsaHI 25 GGCGCC G[GA]^CG[CT]C
307 GGCGCC
BspLU11I 2390 ACATGT A^CATGT
4166 ACATGT
BstXI 2925 CCACAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3026 CCACCTGCGTGG
EcoT22I 3912 ATGCAT ATGCA^T
4391 ATGCAT
Hin1I 25 GGCGCC G[GA]^CG[CT]C
307 GGCGCC
KasI 25 GGCGCC G^GCGCC
307 GGCGCC
NaeI 1876 GCCGGC GCC^GGC
3448 GCCGGC
NarI 25 GGCGCC GG^CGCC
307 GGCGCC
NgoMIV 1876 GCCGGC G^CCGGC
3448 GCCGGC
NsiI 3912 ATGCAT ATGCA^T
4391 ATGCAT
PmaCI 852 CACGTG CAC^GTG
3634 CACGTG
PmlI 852 CACGTG CAC^GTG
3634 CACGTG
PshAI 1895 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2584 GACTGCAGTC
SbfI 967 CCTGCAGG CCTGCA^GG
3549 CCTGCAGG
SfoI 25 GGCGCC GGC^GCC
307 GGCGCC
SmaI 7 CCCGGG CCC^GGG
4103 CCCGGG
SmlI 250 CTCAAG C^T[CT][GA]AG
2086 CTCAAG
SphI 2246 GCATGC GCATG^C
2363 GCATGC
Sse8387I 967 CCTGCAGG CCTGCA^GG
3549 CCTGCAGG
Tsp509I 377 AATT ^AATT
2374 AATT
XmaCI 7 CCCGGG C^CCGGG
4103 CCCGGG
XmaI 7 CCCGGG C^CCGGG
4103 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII