Restriction map for
pF1KSDA0823
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbsI BfrI BglII BlnI
BpuAI BsaBI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 1228 GAATTT [GA]^AATT[CT]
ApaI 89 GGGCCC GGGCC^C
ApoI 1228 GAATTT [GA]^AATT[CT]
AspHI 736 GAGCTC G[AT]GC[AT]^C
BbrPI 10 CACGTG CAC^GTG
BcgI 474 CGACTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 718 GGATAC GTATCC(6/5)
BclI 959 TGATCA T^GATCA
BfaI 857 CTAG C^TAG
BglI 202 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 10 CACGTG [CT]AC^GT[GA]
BsaWI 1290 TCCGGT [AT]^CCGG[AT]
BsiEI 174 CGGCCG CG[GA][CT]^CG
BsiHKAI 736 GAGCTC G[AT]GC[AT]^C
BspHI 962 TCATGA T^CATGA
BssSI 1247 CACGAG CACGAG(-5/-1)
BstEII 1614 GGTCACC G^GT[ACGT]ACC
BstPI 1614 GGTCACC G^GT[ACGT]ACC
BstXI 5 CCAGTCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1247 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 174 CGGCCG C^GGCCG
EarI 913 GAAGAG CTCTTC(1/4)
EclXI 174 CGGCCG C^GGCCG
Eco52I 174 CGGCCG C^GGCCG
EcoO65I 1614 GGTCACC G^GT[ACGT]ACC
FbaI 959 TGATCA T^GATCA
HaeII 83 AGCGCC [GA]GCGC^[CT]
HincII 487 GTCAAC GT[CT]^[GA]AC
HindII 487 GTCAAC GT[CT]^[GA]AC
HinfI 302 GACTC G^A[ACGT]TC
Ksp632I 913 GAAGAG CTCTTC(1/4)
MaeI 857 CTAG C^TAG
MaeII 11 ACGT A^CGT
MseI 1639 TTAA T^TAA
MslI 946 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 509 CATATG CA^TATG
NotI 173 GCGGCCGC GC^GGCCGC
PleI 302 GACTC GAGTC(4/5)
PmaCI 10 CACGTG CAC^GTG
PmlI 10 CACGTG CAC^GTG
RcaI 962 TCATGA T^CATGA
SacI 736 GAGCTC GAGCT^C
SexAI 443 ACCTGGT A^CC[AT]GGT
SfiI 201 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 993 CTTGAG C^T[CT][GA]AG
SphI 798 GCATGC GCATG^C
StuI 221 AGGCCT AGG^CCT
Tru9I 1639 TTAA T^TAA
XcmI 1655 CCATAGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 238 GACGCC G[GA]^CG[CT]C
1390 GACGCC
BcnI 87 CCGGG CC^[GC]GG
1284 CCGGG
BlpI 733 GCTGAGC GC^T[ACGT]AGC
1476 GCTCAGC
Bpu1102I 733 GCTGAGC GC^T[ACGT]AGC
1476 GCTCAGC
BsaHI 238 GACGCC G[GA]^CG[CT]C
1390 GACGCC
BsaI 550 GAGACC GGTCTC(1/5)
1162 GAGACC
BsgI 697 CTGCAC GTGCAG(16/14)
1115 GTGCAG
BsrBI 61 GAGCGG CCGCTC(-3/-3)
154 GAGCGG
BsrDI 290 GCAATG GCAATG(2/0)
1589 GCAATG
BstAPI 123 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1341 GCATGAGGTGC
CelII 733 GCTGAGC GC^T[ACGT]AGC
1476 GCTCAGC
Hin1I 238 GACGCC G[GA]^CG[CT]C
1390 GACGCC
NciI 87 CCGGG CC^[GC]GG
1284 CCGGG
PflMI 657 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1097 CCATCCTGTGG
Tsp509I 345 AATT ^AATT
1229 AATT
Van91I 657 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1097 CCATCCTGTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BanII BbvCI
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII