Restriction map for
pF1KSDA0827
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AvaI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII KasI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 2826 GTCTAC GT^[AC][GT]AC
AcyI 3837 GGCGTC G[GA]^CG[CT]C
AflIII 2621 ACACGT A^C[GA][CT]GT
AhdI 3400 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3400 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2386 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4412 TGGCCA TGG^CCA
BbsI 3189 GTCTTC GAAGAC(2/6)
BbvCI 326 CCTCAGC CCTCAGC(-5/-2)
BcgI 873 GCACCGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4401 TGATCA T^GATCA
BcnI 628 CCGGG CC^[GC]GG
BlpI 24 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTCAGC GC^T[ACGT]AGC
BpuAI 3189 GTCTTC GAAGAC(2/6)
BsaBI 494 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3837 GGCGTC G[GA]^CG[CT]C
BsaI 2017 GAGACC GGTCTC(1/5)
BsgI 2480 GTGCAG GTGCAG(16/14)
BsmBI 3839 CGTCTC CGTCTC(1/5)
BspMI 2916 GCAGGT ACCTGC(4/8)
BssSI 1226 CTCGTG CACGAG(-5/-1)
BstEII 4088 GGTCACC G^GT[ACGT]ACC
BstPI 4088 GGTCACC G^GT[ACGT]ACC
CelII 24 GCTCAGC GC^T[ACGT]AGC
CfoI 29 GCGC GCG^C
DraII 4017 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 700 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1869 GACTACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4412 TGGCCA [CT]^GGCC[GA]
Eam1105I 3400 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 4017 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 4088 GGTCACC G^GT[ACGT]ACC
FbaI 4401 TGATCA T^GATCA
HapII 628 CCGG C^CGG
HgaI 3838 GCGTC GACGC(5/10)
HhaI 29 GCGC GCG^C
Hin1I 3837 GGCGTC G[GA]^CG[CT]C
HinP1I 29 GCGC G^CGC
HindIII 1350 AAGCTT A^AGCTT
HpaII 628 CCGG C^CGG
MaeII 2623 ACGT A^CGT
MamI 494 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4412 TGGCCA TGG^CCA
MscI 4412 TGGCCA TGG^CCA
MspI 628 CCGG C^CGG
NciI 628 CCGGG CC^[GC]GG
NruI 67 TCGCGA TCG^CGA
NspI 4211 ACATGC [GA]CATG^[CT]
PflFI 2386 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4017 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3018 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 878 GAGCTC GAGCT^C
ScaI 2356 AGTACT AGT^ACT
StuI 1034 AGGCCT AGG^CCT
Tth111I 2386 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 30 CGCG CG^CG
68 CGCG
AlwI 1117 GATCC GGATC(4/5)
1885 GGATC
AocI 442 CCTGAGG CC^T[ACGT]AGG
3631 CCTCAGG
AspHI 558 GAGCAC G[AT]GC[AT]^C
878 GAGCTC
BglII 1594 AGATCT A^GATCT
1849 AGATCT
BsiHKAI 558 GAGCAC G[AT]GC[AT]^C
878 GAGCTC
BsmI 1173 GAATGC GAATGC(1/-1)
4349 GCATTC
Bsp1407I 425 TGTACA T^GTACA
855 TGTACA
BsrGI 425 TGTACA T^GTACA
855 TGTACA
BstUI 30 CGCG CG^CG
68 CGCG
BstYI 1594 AGATCT [GA]^GATC[CT]
1849 AGATCT
Bsu36I 442 CCTGAGG CC^T[ACGT]AGG
3631 CCTCAGG
Eco81I 442 CCTGAGG CC^T[ACGT]AGG
3631 CCTCAGG
EcoNI 888 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3249 CCTTTCCCAGG
FauI 3609 CCCGC CCCGC(4/6)
748 GCGGG
HpaI 369 GTTAAC GTT^AAC
3061 GTTAAC
MflI 1594 AGATCT [GA]^GATC[CT]
1849 AGATCT
MslI 2934 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3366 CATTGCTGTG
MvnI 30 CGCG CG^CG
68 CGCG
SspBI 425 TGTACA T^GTACA
855 TGTACA
SspI 1756 AATATT AAT^ATT
2278 AATATT
XbaI 118 TCTAGA T^CTAGA
2152 TCTAGA
XcmI 771 CCAAAAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3824 CCATGGCTACAATGG
XhoII 1594 AGATCT [GA]^GATC[CT]
1849 AGATCT
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
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Acc65I AciI AcsI AfaI AluI
AlwNI ApoI Asp700I Asp718I AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI KpnI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI