Restriction map for
pF1KSDA0880
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# NO CUT ENZYMES ( 115 enzymes) #
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AccI AccII AccIII AclI AcsI
AflII AflIII AhdI ApoI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 255 GACGTC GACGT^C
AgeI 1946 ACCGGT A^CCGGT
Aor51HI 233 AGCGCT AGC^GCT
Asp700I 231 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1169 TGGCCA TGG^CCA
BcnI 330 CCGGG CC^[GC]GG
BglI 1547 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1946 ACCGGT [AT]^CCGG[AT]
BseRI 1763 GAGGAG GAGGAG(10/8)
BspDI 640 ATCGAT AT^CGAT
BsrBI 1375 CCGCTC CCGCTC(-3/-3)
BssSI 1523 CTCGTG CACGAG(-5/-1)
BstAPI 2076 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1184 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 640 ATCGAT AT^CGAT
Eco47III 233 AGCGCT AGC^GCT
EcoNI 1131 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MfeI 2080 CAATTG C^AATTG
MluNI 1169 TGGCCA TGG^CCA
MscI 1169 TGGCCA TGG^CCA
MunI 2080 CAATTG C^AATTG
NciI 330 CCGGG CC^[GC]GG
NcoI 1154 CCATGG C^CATGG
PinAI 1946 ACCGGT A^CCGGT
SacI 210 GAGCTC GAGCT^C
SapI 1377 GCTCTTC GCTCTTC(1/4)
TfiI 2116 GATTC G^A[AT]TC
Tsp509I 2081 AATT ^AATT
XmnI 231 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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Acc65I 30 GGTACC G^GTACC
39 GGTACC
AfaI 31 GTAC GT^AC
40 GTAC
Alw44I 334 GTGCAC G^TGCAC
1879 GTGCAC
ApaI 1311 GGGCCC GGGCC^C
1886 GGGCCC
ApaLI 334 GTGCAC G^TGCAC
1879 GTGCAC
Asp718I 30 GGTACC G^GTACC
39 GGTACC
AvaI 679 CTCGGG C^[CT]CG[GA]G
2120 CCCGAG
BamHI 683 GGATCC G^GATCC
1651 GGATCC
BbsI 1777 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BcgI 642 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1856 GCAGCGCCATCG
BfaI 1030 CTAG C^TAG
2086 CTAG
BpuAI 1777 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BsgI 854 GTGCAG GTGCAG(16/14)
1668 GTGCAG
BsmAI 1455 GTCTC GTCTC(1/5)
1727 GTCTC
BsmFI 3 GGGAC GGGAC(10/14)
1134 GTCCC
BsoBI 679 CTCGGG C^[CT]CG[GA]G
2120 CCCGAG
BspHI 188 TCATGA T^CATGA
392 TCATGA
BsrFI 850 GCCGGT [GA]^CCGG[CT]
1946 ACCGGT
Cfr10I 850 GCCGGT [GA]^CCGG[CT]
1946 ACCGGT
DsaI 1154 CCATGG C^C[GA][CT]GG
1851 CCACGG
EaeI 121 CGGCCA [CT]^GGCC[GA]
1169 TGGCCA
EarI 1360 CTCTTC CTCTTC(1/4)
1378 CTCTTC
HgaI 1286 GCGTC GACGC(5/10)
1902 GCGTC
KpnI 30 GGTACC GGTAC^C
39 GGTACC
Ksp632I 1360 CTCTTC CTCTTC(1/4)
1378 CTCTTC
MaeI 1030 CTAG C^TAG
2086 CTAG
MaeIII 697 GTGAC ^GT[ACGT]AC
949 GTCAC
PflMI 775 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCATCTGGTGG
PpuMI 3 GGGACCC [GA]G^G[AT]CC[CT]
803 AGGACCC
PvuII 175 CAGCTG CAG^CTG
1617 CAGCTG
RcaI 188 TCATGA T^CATGA
392 TCATGA
RsaI 31 GTAC GT^AC
40 GTAC
SmlI 531 CTCAAG C^T[CT][GA]AG
1560 CTCAAG
StuI 714 AGGCCT AGG^CCT
1472 AGGCCT
Tsp45I 697 GTGAC ^GT[GC]AC
949 GTCAC
Van91I 775 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCATCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AocI AspHI AvaII BanI BanII
BbvI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI TspRI
TthHB8I XcmI XhoII