Restriction map for
pF1KSDA0891
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII AclI AflII AgeI AscI
AseI AsnI AviII BclI BfrI
BlpI Bpu1102I BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FspI HincII HindII HpaI
KspI MfeI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SnaBI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 2921 GGTACC G^GTACC
AccI 3586 GTAGAC GT^[AC][GT]AC
AccII 3608 CGCG CG^CG
AccIII 743 TCCGGA T^CCGGA
AhdI 2245 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2828 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2921 GGTACC G^GTACC
AspEI 2245 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3132 GGATCC G^GATCC
BbeI 3290 GGCGCC GGCGC^C
BciVI 3934 GTATCC GTATCC(6/5)
BglII 914 AGATCT A^GATCT
BsaBI 106 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 743 TCCGGA T^CCGGA
BspEI 743 TCCGGA T^CCGGA
BstEII 2745 GGTGACC G^GT[ACGT]ACC
BstPI 2745 GGTGACC G^GT[ACGT]ACC
BstUI 3608 CGCG CG^CG
Eam1105I 2245 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2745 GGTGACC G^GT[ACGT]ACC
EcoT22I 1395 ATGCAT ATGCA^T
FseI 3162 GGCCGGCC GGCCGG^CC
HindIII 388 AAGCTT A^AGCTT
KasI 3290 GGCGCC G^GCGCC
KpnI 2921 GGTACC GGTAC^C
MamI 106 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3607 ACGCGT A^CGCGT
MroI 743 TCCGGA T^CCGGA
MseI 2201 TTAA T^TAA
MvnI 3608 CGCG CG^CG
NarI 3290 GGCGCC GG^CGCC
NsiI 1395 ATGCAT ATGCA^T
PshAI 2800 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2734 GAGCTC GAGCT^C
ScaI 936 AGTACT AGT^ACT
SfoI 3290 GGCGCC GGC^GCC
SgrAI 3634 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 61 ACTAGT A^CTAGT
Tru9I 2201 TTAA T^TAA
XmnI 2828 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 675 GAATTT [GA]^AATT[CT]
2828 GAATTT
AcyI 1631 GGCGTC G[GA]^CG[CT]C
3290 GGCGCC
ApoI 675 GAATTT [GA]^AATT[CT]
2828 GAATTT
AspI 126 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
721 GACCCAGTC
AvrII 115 CCTAGG C^CTAGG
3696 CCTAGG
BbsI 307 GAAGAC GAAGAC(2/6)
493 GTCTTC
BcgI 3447 CGATCTATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
75 GCAGAAGGATCG
BlnI 115 CCTAGG C^CTAGG
3696 CCTAGG
BpuAI 307 GAAGAC GAAGAC(2/6)
493 GTCTTC
BsaHI 1631 GGCGTC G[GA]^CG[CT]C
3290 GGCGCC
BsaWI 743 TCCGGA [AT]^CCGG[AT]
3866 TCCGGT
BsmI 2946 GAATGC GAATGC(1/-1)
1817 GCATTC
BspHI 315 TCATGA T^CATGA
1520 TCATGA
BsrBI 1581 CCGCTC CCGCTC(-3/-3)
2308 GAGCGG
BstXI 198 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3110 CCAAGGAGCTGG
Hin1I 1631 GGCGTC G[GA]^CG[CT]C
3290 GGCGCC
MslI 1335 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2929 CATCCAAGTG
NaeI 3163 GCCGGC GCC^GGC
3635 GCCGGC
NcoI 737 CCATGG C^CATGG
1340 CCATGG
NgoMIV 3163 GCCGGC G^CCGGC
3635 GCCGGC
NspI 1347 GCATGT [GA]CATG^[CT]
1393 ACATGC
PflFI 126 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
721 GACCCAGTC
PflMI 1423 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2235 CCACTCACTGG
RcaI 315 TCATGA T^CATGA
1520 TCATGA
StuI 3230 AGGCCT AGG^CCT
3266 AGGCCT
Tth111I 126 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
721 GACCCAGTC
Van91I 1423 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2235 CCACTCACTGG
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI