Restriction map for
pF1KSDA0959
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AvrII BalI BamHI BbeI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco52I EcoO65I EcoT22I FseI HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1890 GACGTC GACGT^C
AccI 956 GTAGAC GT^[AC][GT]AC
AflIII 1985 ACATGT A^C[GA][CT]GT
AhdI 164 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1802 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1874 AGCGCT AGC^GCT
AspEI 164 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1654 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2189 TGCGCA TGC^GCA
BbrPI 684 CACGTG CAC^GTG
BbvCI 1696 GCTGAGG CCTCAGC(-5/-2)
BcgI 1904 CGACTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 538 CCGGG CC^[GC]GG
BglII 699 AGATCT A^GATCT
BsaAI 684 CACGTG [CT]AC^GT[GA]
BsaBI 936 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 782 GGTCTC GGTCTC(1/5)
Bsp1407I 1988 TGTACA T^GTACA
BspHI 948 TCATGA T^CATGA
BspLU11I 1985 ACATGT A^CATGT
BsrGI 1988 TGTACA T^GTACA
BssSI 703 CTCGTG CACGAG(-5/-1)
BstYI 699 AGATCT [GA]^GATC[CT]
Bsu36I 1802 CCTCAGG CC^T[ACGT]AGG
DraII 445 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 164 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1874 AGCGCT AGC^GCT
Eco81I 1802 CCTCAGG CC^T[ACGT]AGG
EcoO109I 445 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 679 GAATTC G^AATTC
EcoRV 1087 GATATC GAT^ATC
FspI 2189 TGCGCA TGC^GCA
HaeII 1874 AGCGCT [GA]GCGC^[CT]
HgaI 1507 GACGC GACGC(5/10)
HincII 2004 GTTGAC GT[CT]^[GA]AC
HindII 2004 GTTGAC GT[CT]^[GA]AC
HindIII 226 AAGCTT A^AGCTT
MamI 936 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 699 AGATCT [GA]^GATC[CT]
NciI 538 CCGGG CC^[GC]GG
NspI 1985 ACATGT [GA]CATG^[CT]
PflFI 1654 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 684 CACGTG CAC^GTG
PmlI 684 CACGTG CAC^GTG
RcaI 948 TCATGA T^CATGA
SspBI 1988 TGTACA T^GTACA
Tth111I 1654 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1709 CCATTACTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 699 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 1507 GACGCC G[GA]^CG[CT]C
1890 GACGTC
BanI 214 GGCACC G^G[CT][GA]CC
1261 GGCACC
BclI 416 TGATCA T^GATCA
1583 TGATCA
BfaI 133 CTAG C^TAG
1570 CTAG
BsaHI 1507 GACGCC G[GA]^CG[CT]C
1890 GACGTC
BsgI 464 GTGCAG GTGCAG(16/14)
2095 GTGCAG
BsmI 423 GAATGC GAATGC(1/-1)
1061 GAATGC
CfoI 1875 GCGC GCG^C
2190 GCGC
FbaI 416 TGATCA T^GATCA
1583 TGATCA
HaeIII 447 GGCC GG^CC
987 GGCC
HapII 538 CCGG C^CGG
2232 CCGG
HhaI 1875 GCGC GCG^C
2190 GCGC
Hin1I 1507 GACGCC G[GA]^CG[CT]C
1890 GACGTC
HinP1I 1875 GCGC G^CGC
2190 GCGC
HpaII 538 CCGG C^CGG
2232 CCGG
MaeI 133 CTAG C^TAG
1570 CTAG
MspI 538 CCGG C^CGG
2232 CCGG
PflMI 762 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1782 CCATTCCATGG
PstI 1003 CTGCAG CTGCA^G
1210 CTGCAG
SacI 27 GAGCTC GAGCT^C
1635 GAGCTC
SapI 557 GAAGAGC GCTCTTC(1/4)
1536 GAAGAGC
SfcI 1003 CTGCAG C^T[GA][CT]AG
1210 CTGCAG
SmlI 574 CTTGAG C^T[CT][GA]AG
981 CTTGAG
Tsp45I 79 GTCAC ^GT[GC]AC
1888 GTGAC
Van91I 762 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1782 CCATTCCATGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I