Restriction map for
pF1KSDA1001
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BseRI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NarI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AgeI 1300 ACCGGT A^CCGGT
AlwNI 1388 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 1019 GGCACC G^G[CT][GA]CC
BbrPI 751 CACGTG CAC^GTG
BbsI 1375 GAAGAC GAAGAC(2/6)
BglI 281 GCCGGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1375 GAAGAC GAAGAC(2/6)
BsaAI 751 CACGTG [CT]AC^GT[GA]
BsaI 352 GAGACC GGTCTC(1/5)
BsaWI 1300 ACCGGT [AT]^CCGG[AT]
BsgI 809 GTGCAG GTGCAG(16/14)
BsiEI 279 CGGCCG CG[GA][CT]^CG
BsmBI 1170 CGTCTC CGTCTC(1/5)
BspMI 247 ACCTGC ACCTGC(4/8)
BsrBI 343 CCGCTC CCGCTC(-3/-3)
BstAPI 1206 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 145 GGTGACC G^GT[ACGT]ACC
BstF5I 690 CATCC GGATG(2/0)
BstPI 145 GGTGACC G^GT[ACGT]ACC
DpnII 846 GATC ^GATC
EagI 279 CGGCCG C^GGCCG
EclXI 279 CGGCCG C^GGCCG
Eco52I 279 CGGCCG C^GGCCG
EcoNI 1122 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 145 GGTGACC G^GT[ACGT]ACC
EcoT14I 359 CCTTGG C^C[AT][AT]GG
FokI 690 CATCC GGATG(9/13)
HincII 859 GTTGAC GT[CT]^[GA]AC
HindII 859 GTTGAC GT[CT]^[GA]AC
MboI 846 GATC ^GATC
MboII 1375 GAAGA GAAGA(8/7)
NaeI 281 GCCGGC GCC^GGC
NdeII 846 GATC ^GATC
NgoMIV 281 GCCGGC G^CCGGC
NheI 933 GCTAGC G^CTAGC
NspI 746 ACATGC [GA]CATG^[CT]
PflMI 486 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1300 ACCGGT A^CCGGT
PmaCI 751 CACGTG CAC^GTG
PmlI 751 CACGTG CAC^GTG
Sau3AI 846 GATC ^GATC
SfiI 280 GGCCGGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 260 CCCGGG CCC^GGG
StyI 359 CCTTGG C^C[AT][AT]GG
TfiI 473 GAATC G^A[AT]TC
Tsp509I 1551 AATT ^AATT
Van91I 486 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 260 CCCGGG C^CCGGG
XmaI 260 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 1168 GACGTC GACGT^C
1462 GACGTC
AcyI 1168 GACGTC G[GA]^CG[CT]C
1462 GACGTC
AfaI 500 GTAC GT^AC
1422 GTAC
AspHI 1180 GTGCTC G[AT]GC[AT]^C
1326 GAGCAC
AvaI 260 CCCGGG C^[CT]CG[GA]G
1438 CCCGAG
BfaI 934 CTAG C^TAG
1402 CTAG
BsaHI 1168 GACGTC G[GA]^CG[CT]C
1462 GACGTC
BsiHKAI 1180 GTGCTC G[AT]GC[AT]^C
1326 GAGCAC
BsmFI 950 GTCCC GGGAC(10/14)
1027 GTCCC
BsoBI 260 CCCGGG C^[CT]CG[GA]G
1438 CCCGAG
BsrDI 1476 CATTGC GCAATG(2/0)
296 GCAATG
CfoI 784 GCGC GCG^C
1152 GCGC
HaeII 783 AGCGCC [GA]GCGC^[CT]
1151 GGCGCT
HhaI 784 GCGC GCG^C
1152 GCGC
Hin1I 1168 GACGTC G[GA]^CG[CT]C
1462 GACGTC
HinP1I 784 GCGC G^CGC
1152 GCGC
MaeI 934 CTAG C^TAG
1402 CTAG
MseI 1064 TTAA T^TAA
1087 TTAA
PleI 768 GACTC GAGTC(4/5)
302 GAGTC
PvuII 236 CAGCTG CAG^CTG
1238 CAGCTG
RsaI 500 GTAC GT^AC
1422 GTAC
SfaNI 77 GCATC GCATC(5/9)
414 GCATC
SmlI 645 CTTGAG C^T[CT][GA]AG
1142 CTCAAG
StuI 336 AGGCCT AGG^CCT
1286 AGGCCT
Tru9I 1064 TTAA T^TAA
1087 TTAA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstNI Cac8I Cfr10I Cfr13I
DdeI DraII DraIII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PpuMI PstI Sau96I ScrFI
SfcI TseI Tsp45I TspRI