Restriction map for
pF1KSDA1027
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# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I AvrII BbrPI BciVI
BclI BlnI BsaBI BsiEI BsiWI
BssHII Bst1107I BstBI BstZ17I DraI
EagI EclXI Eco52I EcoRI EcoT22I
FbaI HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SacII SalI SbfI SfuI
SgrAI SnaBI Sse8387I SwaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 3423 GACGTC GACGT^C
AccIII 3101 TCCGGA T^CCGGA
AcsI 1383 GAATTT [GA]^AATT[CT]
AflIII 4157 ACATGT A^C[GA][CT]GT
AhdI 1264 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1383 GAATTT [GA]^AATT[CT]
AspEI 1264 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2503 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 104 GGATCC G^GATCC
BbeI 5803 GGCGCC GGCGC^C
BsaAI 892 TACGTG [CT]AC^GT[GA]
BsaWI 3101 TCCGGA [AT]^CCGG[AT]
BseAI 3101 TCCGGA T^CCGGA
BspDI 1186 ATCGAT AT^CGAT
BspEI 3101 TCCGGA T^CCGGA
BspLU11I 4157 ACATGT A^CATGT
ClaI 1186 ATCGAT AT^CGAT
CpoI 4115 CGGTCCG CG^G[AT]CCG
Eam1105I 1264 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1189 GATATC GAT^ATC
FseI 4899 GGCCGGCC GGCCGG^CC
KasI 5803 GGCGCC G^GCGCC
MroI 3101 TCCGGA T^CCGGA
NarI 5803 GGCGCC GG^CGCC
NheI 900 GCTAGC G^CTAGC
PflFI 2503 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 523 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4115 CGGTCCG CG^G[AT]CCG
SapI 7441 GAAGAGC GCTCTTC(1/4)
ScaI 5207 AGTACT AGT^ACT
SexAI 2504 ACCTGGT A^CC[AT]GGT
SfiI 5688 GGCCTCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5803 GGCGCC GGC^GCC
SspI 377 AATATT AAT^ATT
Tth111I 2503 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3177 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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Alw44I 4056 GTGCAC G^TGCAC
7070 GTGCAC
Aor51HI 2941 AGCGCT AGC^GCT
5947 AGCGCT
ApaLI 4056 GTGCAC G^TGCAC
7070 GTGCAC
Asp700I 786 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7519 GAAATGCTTC
BcgI 4311 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3771 GCAGGCCTCTCG
BsmI 2718 GAATGC GAATGC(1/-1)
4748 GCATTC
BspHI 335 TCATGA T^CATGA
369 TCATGA
BssSI 562 CACGAG CACGAG(-5/-1)
656 CACGAG
Eco47III 2941 AGCGCT AGC^GCT
5947 AGCGCT
HincII 1284 GTCAAC GT[CT]^[GA]AC
6804 GTTGAC
HindII 1284 GTCAAC GT[CT]^[GA]AC
6804 GTTGAC
PaeR7I 3212 CTCGAG C^TCGAG
3803 CTCGAG
RcaI 335 TCATGA T^CATGA
369 TCATGA
SpeI 2145 ACTAGT A^CTAGT
7107 ACTAGT
XhoI 3212 CTCGAG C^TCGAG
3803 CTCGAG
XmnI 786 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7519 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI