Restriction map for
pF1KSDA1098
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbrPI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BspEI BspLU11I
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CpoI
DraI DraII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tsp509I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 184 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1403 GTGCAC G^TGCAC
ApaLI 1403 GTGCAC G^TGCAC
AspEI 184 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1403 GTGCAC G[AT]GC[AT]^C
AspI 88 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1265 TGGCCA TGG^CCA
BamHI 557 GGATCC G^GATCC
BbsI 1048 GTCTTC GAAGAC(2/6)
BbvCI 1141 GCTGAGG CCTCAGC(-5/-2)
BlpI 321 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 321 GCTCAGC GC^T[ACGT]AGC
BpuAI 1048 GTCTTC GAAGAC(2/6)
BsaAI 1118 TACGTG [CT]AC^GT[GA]
BsiHKAI 1403 GTGCAC G[AT]GC[AT]^C
BsmBI 688 GAGACG CGTCTC(1/5)
Bsp1407I 1349 TGTACA T^GTACA
BspDI 832 ATCGAT AT^CGAT
BspHI 758 TCATGA T^CATGA
BsrFI 405 GCCGGT [GA]^CCGG[CT]
BsrGI 1349 TGTACA T^GTACA
BstYI 557 GGATCC [GA]^GATC[CT]
CelII 321 GCTCAGC GC^T[ACGT]AGC
Cfr10I 405 GCCGGT [GA]^CCGG[CT]
ClaI 832 ATCGAT AT^CGAT
Eam1105I 184 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 577 AAGCTT A^AGCTT
MflI 557 GGATCC [GA]^GATC[CT]
MluNI 1265 TGGCCA TGG^CCA
MscI 1265 TGGCCA TGG^CCA
MslI 1450 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 582 TCGCGA TCG^CGA
PaeR7I 337 CTCGAG C^TCGAG
PflFI 88 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 758 TCATGA T^CATGA
SexAI 230 ACCTGGT A^CC[AT]GGT
SfiI 480 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 25 CCCGGG CCC^GGG
SspBI 1349 TGTACA T^GTACA
StuI 479 AGGCCT AGG^CCT
TfiI 898 GAATC G^A[AT]TC
Tth111I 88 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 337 CTCGAG C^TCGAG
XhoII 557 GGATCC [GA]^GATC[CT]
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1023 GGCGCC G[GA]^CG[CT]C
1411 GGCGCC
AflIII 17 ACGTGT A^C[GA][CT]GT
1119 ACGTGT
AlwI 558 GATCC GGATC(4/5)
557 GGATC
AviII 206 TGCGCA TGC^GCA
1433 TGCGCA
BbeI 1023 GGCGCC GGCGC^C
1411 GGCGCC
BcgI 784 CGACTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
824 GCATGCTTATCG
BglI 481 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1031 GCCTGCTGGGC
BsaHI 1023 GGCGCC G[GA]^CG[CT]C
1411 GGCGCC
BsmAI 634 GAGAC GTCTC(1/5)
688 GAGAC
BssHII 266 GCGCGC G^CGCGC
268 GCGCGC
BstAPI 393 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
456 GCATGCACTGC
DdeI 322 CTCAG C^T[ACGT]AG
1142 CTGAG
DpnII 558 GATC ^GATC
711 GATC
DraIII 394 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1225 CACTGCGTG
EarI 328 CTCTTC CTCTTC(1/4)
787 CTCTTC
FspI 206 TGCGCA TGC^GCA
1433 TGCGCA
Hin1I 1023 GGCGCC G[GA]^CG[CT]C
1411 GGCGCC
KasI 1023 GGCGCC G^GCGCC
1411 GGCGCC
Ksp632I 328 CTCTTC CTCTTC(1/4)
787 CTCTTC
KspI 123 CCGCGG CCGC^GG
312 CCGCGG
MboI 558 GATC ^GATC
711 GATC
NarI 1023 GGCGCC GG^CGCC
1411 GGCGCC
NdeII 558 GATC ^GATC
711 GATC
NspI 456 GCATGC [GA]CATG^[CT]
824 GCATGC
PvuII 932 CAGCTG CAG^CTG
1158 CAGCTG
SacII 123 CCGCGG CCGC^GG
312 CCGCGG
Sau3AI 558 GATC ^GATC
711 GATC
SfoI 1023 GGCGCC GGC^GCC
1411 GGCGCC
SmlI 337 CTCGAG C^T[CT][GA]AG
591 CTTGAG
SphI 456 GCATGC GCATG^C
824 GCATGC
XcmI 1247 CCACGTCGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1359 CCACGCCCGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
PleI PstI RsaI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I