Restriction map for
pF1KSDA1219
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccII AclI AgeI AhdI Aor51HI
ApaI AscI AspEI AviII AvrII
BamHI BbrPI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HgaI
KspI MamI MfeI MluI MunI
MvnI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1236 GACGTC GACGT^C
Acc65I 136 GGTACC G^GTACC
AccI 3377 GTCTAC GT^[AC][GT]AC
AccIII 4196 TCCGGA T^CCGGA
AflIII 863 ACATGT A^C[GA][CT]GT
AocI 3976 CCTCAGG CC^T[ACGT]AGG
Asp718I 136 GGTACC G^GTACC
AspI 4384 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 476 CCCGAG C^[CT]CG[GA]G
BalI 1679 TGGCCA TGG^CCA
BbeI 2309 GGCGCC GGCGC^C
BbvCI 1540 GCTGAGG CCTCAGC(-5/-2)
BciVI 972 GTATCC GTATCC(6/5)
BclI 42 TGATCA T^GATCA
BseAI 4196 TCCGGA T^CCGGA
BsoBI 476 CCCGAG C^[CT]CG[GA]G
BspEI 4196 TCCGGA T^CCGGA
BspLU11I 863 ACATGT A^CATGT
BsrBI 945 GAGCGG CCGCTC(-3/-3)
Bsu36I 3976 CCTCAGG CC^T[ACGT]AGG
DraII 428 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 2732 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3976 CCTCAGG CC^T[ACGT]AGG
EcoO109I 428 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 42 TGATCA T^GATCA
HaeII 2309 GGCGCC [GA]GCGC^[CT]
HindIII 2995 AAGCTT A^AGCTT
HpaI 1318 GTTAAC GTT^AAC
KasI 2309 GGCGCC G^GCGCC
KpnI 136 GGTACC GGTAC^C
MluNI 1679 TGGCCA TGG^CCA
MroI 4196 TCCGGA T^CCGGA
MscI 1679 TGGCCA TGG^CCA
NarI 2309 GGCGCC GG^CGCC
NdeI 755 CATATG CA^TATG
PflFI 4384 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 229 CCATTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 428 AGGTCCT [GA]G^G[AT]CC[CT]
SfoI 2309 GGCGCC GGC^GCC
SpeI 1273 ACTAGT A^CTAGT
SwaI 3042 ATTTAAAT ATTT^AAAT
Tth111I 4384 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 229 CCATTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1236 GACGTC G[GA]^CG[CT]C
2309 GGCGCC
AflII 2232 CTTAAG C^TTAAG
2557 CTTAAG
Alw44I 725 GTGCAC G^TGCAC
2418 GTGCAC
ApaLI 725 GTGCAC G^TGCAC
2418 GTGCAC
Asp700I 1591 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4054 GAAAACTTTC
BcgI 789 CGATGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2273 GCATTCATCTCG
BfrI 2232 CTTAAG C^TTAAG
2557 CTTAAG
BglII 3108 AGATCT A^GATCT
4132 AGATCT
BsaHI 1236 GACGTC G[GA]^CG[CT]C
2309 GGCGCC
BsgI 64 CTGCAC GTGCAG(16/14)
2473 CTGCAC
CfoI 2310 GCGC GCG^C
2720 GCGC
DsaI 197 CCATGG C^C[GA][CT]GG
1478 CCATGG
EcoNI 2358 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3935 CCTCACAGAGG
EcoRI 3298 GAATTC G^AATTC
3872 GAATTC
EcoT22I 1040 ATGCAT ATGCA^T
2700 ATGCAT
FauI 3282 CCCGC CCCGC(4/6)
3311 CCCGC
HhaI 2310 GCGC GCG^C
2720 GCGC
Hin1I 1236 GACGTC G[GA]^CG[CT]C
2309 GGCGCC
HinP1I 2310 GCGC G^CGC
2720 GCGC
MaeII 1237 ACGT A^CGT
3035 ACGT
NcoI 197 CCATGG C^CATGG
1478 CCATGG
NheI 1432 GCTAGC G^CTAGC
3715 GCTAGC
NsiI 1040 ATGCAT ATGCA^T
2700 ATGCAT
PstI 511 CTGCAG CTGCA^G
4464 CTGCAG
SacI 2344 GAGCTC GAGCT^C
4274 GAGCTC
SapI 1535 GAAGAGC GCTCTTC(1/4)
1937 GCTCTTC
TaqI 414 TCGA T^CGA
1446 TCGA
TthHB8I 414 TCGA T^CGA
1446 TCGA
XbaI 1057 TCTAGA T^CTAGA
2340 TCTAGA
XmnI 1591 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4054 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII