Restriction map for
pF1KSDA1379
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MflI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AlwNI 51 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1207 GAGCTC G[AT]GC[AT]^C
BalI 506 TGGCCA TGG^CCA
BanII 1207 GAGCTC G[GA]GC[CT]^C
BlpI 1209 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1209 GCTCAGC GC^T[ACGT]AGC
BsaAI 1318 TACGTG [CT]AC^GT[GA]
BsiHKAI 1207 GAGCTC G[AT]GC[AT]^C
BsiWI 164 CGTACG C^GTACG
BsmFI 1025 GGGAC GGGAC(10/14)
BspDI 94 ATCGAT AT^CGAT
BspMI 684 GCAGGT ACCTGC(4/8)
BsrBI 241 GAGCGG CCGCTC(-3/-3)
BsrDI 696 GCAATG GCAATG(2/0)
BssHII 144 GCGCGC G^CGCGC
BstAPI 597 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1313 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1209 GCTCAGC GC^T[ACGT]AGC
ClaI 94 ATCGAT AT^CGAT
CpoI 79 CGGACCG CG^G[AT]CCG
DraII 323 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1071 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 323 AGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 355 GACGC GACGC(5/10)
KspI 1029 CCGCGG CCGC^GG
MaeII 1319 ACGT A^CGT
MaeIII 547 GTCAC ^GT[ACGT]AC
MluNI 506 TGGCCA TGG^CCA
MscI 506 TGGCCA TGG^CCA
MseI 1217 TTAA T^TAA
NspI 881 GCATGC [GA]CATG^[CT]
PpuMI 323 AGGACCT [GA]G^G[AT]CC[CT]
PstI 571 CTGCAG CTGCA^G
PvuII 1294 CAGCTG CAG^CTG
RsrII 79 CGGACCG CG^G[AT]CCG
SacI 1207 GAGCTC GAGCT^C
SacII 1029 CCGCGG CCGC^GG
SfcI 571 CTGCAG C^T[GA][CT]AG
SmlI 733 CTCAAG C^T[CT][GA]AG
SphI 881 GCATGC GCATG^C
Tru9I 1217 TTAA T^TAA
Tsp45I 547 GTCAC ^GT[GC]AC
Tsp509I 707 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BcnI 825 CCGGG CC^[GC]GG
877 CCCGG
BmyI 1172 GGGCAC G[AGT]GC[ACT]^C
1207 GAGCTC
BsaI 40 GAGACC GGTCTC(1/5)
1117 GAGACC
BsmBI 391 GAGACG CGTCTC(1/5)
1226 GAGACG
Bsp1286I 1172 GGGCAC G[AGT]GC[ACT]^C
1207 GAGCTC
DpnII 153 GATC ^GATC
372 GATC
DsaI 1029 CCGCGG C^C[GA][CT]GG
1272 CCGTGG
EcoNI 327 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
732 CCTCAAGGAGG
EcoT14I 433 CCTTGG C^C[AT][AT]GG
1154 CCAAGG
HinfI 1006 GAGTC G^A[ACGT]TC
1150 GACTC
MboI 153 GATC ^GATC
372 GATC
MslI 792 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
882 CATGCCCATG
NciI 825 CCGGG CC^[GC]GG
877 CCCGG
NdeII 153 GATC ^GATC
372 GATC
PleI 1150 GACTC GAGTC(4/5)
1006 GAGTC
Sau3AI 153 GATC ^GATC
372 GATC
SfaNI 835 GATGC GCATC(5/9)
92 GCATC
StuI 20 AGGCCT AGG^CCT
473 AGGCCT
StyI 433 CCTTGG C^C[AT][AT]GG
1154 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbeI BbsI BbvI
BglI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NlaIII NlaIV PflMI
RsaI Sau96I ScrFI SfoI TaqI
TseI TspRI TthHB8I Van91I