Restriction map for
pF1KSDA1400
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AhdI AocI Aor51HI ApoI AscI
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BciVI BlnI BsaI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoT22I FseI
HindIII KpnI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 2509 CTTAAG C^TTAAG
AflIII 1406 ACGTGT A^C[GA][CT]GT
AgeI 2572 ACCGGT A^CCGGT
Alw44I 586 GTGCAC G^TGCAC
ApaI 999 GGGCCC GGGCC^C
ApaLI 586 GTGCAC G^TGCAC
AseI 343 ATTAAT AT^TAAT
AsnI 343 ATTAAT AT^TAAT
Asp700I 1608 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 530 GCAACCGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2763 TGATCA T^GATCA
BfrI 2509 CTTAAG C^TTAAG
BglI 1717 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 117 AGATCT A^GATCT
BsaBI 2758 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2572 ACCGGT [AT]^CCGG[AT]
BsiEI 1464 CGACCG CG[GA][CT]^CG
BsmBI 1196 GAGACG CGTCTC(1/5)
BspHI 2766 TCATGA T^CATGA
BssHII 2329 GCGCGC G^CGCGC
BssSI 802 CTCGTG CACGAG(-5/-1)
BstAPI 579 GCAAGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1789 GGTTACC G^GT[ACGT]ACC
BstPI 1789 GGTTACC G^GT[ACGT]ACC
EcoNI 1729 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1789 GGTTACC G^GT[ACGT]ACC
EcoRV 2596 GATATC GAT^ATC
FbaI 2763 TGATCA T^GATCA
HpaI 2683 GTTAAC GTT^AAC
KspI 605 CCGCGG CCGC^GG
MamI 2758 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2814 CAATTG C^AATTG
MunI 2814 CAATTG C^AATTG
NcoI 1986 CCATGG C^CATGG
NspI 2919 GCATGT [GA]CATG^[CT]
PaeR7I 1507 CTCGAG C^TCGAG
PflMI 1653 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2572 ACCGGT A^CCGGT
PshAI 2728 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 343 ATTAAT AT^TAAT
PstI 2517 CTGCAG CTGCA^G
RcaI 2766 TCATGA T^CATGA
SacII 605 CCGCGG CCGC^GG
ScaI 1041 AGTACT AGT^ACT
StuI 666 AGGCCT AGG^CCT
TfiI 536 GATTC G^A[AT]TC
Van91I 1653 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1507 CTCGAG C^TCGAG
XmnI 1608 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 760 GTCTAC GT^[AC][GT]AC
1399 GTCTAC
AlwNI 1654 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2945 CAGACACTG
AviII 1017 TGCGCA TGC^GCA
1913 TGCGCA
BalI 2192 TGGCCA TGG^CCA
2255 TGGCCA
BbsI 1218 GTCTTC GAAGAC(2/6)
1534 GTCTTC
BlpI 541 GCTGAGC GC^T[ACGT]AGC
2652 GCTCAGC
Bpu1102I 541 GCTGAGC GC^T[ACGT]AGC
2652 GCTCAGC
BpuAI 1218 GTCTTC GAAGAC(2/6)
1534 GTCTTC
BsmI 436 GCATTC GAATGC(1/-1)
2695 GCATTC
CelII 541 GCTGAGC GC^T[ACGT]AGC
2652 GCTCAGC
DrdI 751 GACCAACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2475 GACCCCTGAGTC
EarI 2806 CTCTTC CTCTTC(1/4)
955 GAAGAG
FspI 1017 TGCGCA TGC^GCA
1913 TGCGCA
Ksp632I 2806 CTCTTC CTCTTC(1/4)
955 GAAGAG
MluNI 2192 TGGCCA TGG^CCA
2255 TGGCCA
MscI 2192 TGGCCA TGG^CCA
2255 TGGCCA
PpuMI 178 AGGACCC [GA]G^G[AT]CC[CT]
992 AGGACCT
SacI 2373 GAGCTC GAGCT^C
2650 GAGCTC
SfcI 1295 CTGTAG C^T[GA][CT]AG
2517 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
SmaI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI