Restriction map for
pF1KSDA1436
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# NO CUT ENZYMES ( 80 enzymes) #
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Acc65I AccI AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI HindIII
KpnI MamI MfeI MluI MluNI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 922 GACGTC GACGT^C
AccIII 1499 TCCGGA T^CCGGA
AcsI 2323 GAATTC [GA]^AATT[CT]
AlwNI 1149 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2054 CCTCAGG CC^T[ACGT]AGG
Aor51HI 638 AGCGCT AGC^GCT
ApoI 2323 GAATTC [GA]^AATT[CT]
Asp700I 2478 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 939 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 5 GGCGCC GGCGC^C
BbvCI 2295 CCTCAGC CCTCAGC(-5/-2)
BciVI 2490 GTATCC GTATCC(6/5)
BclI 2117 TGATCA T^GATCA
BfaI 1210 CTAG C^TAG
BglII 2112 AGATCT A^GATCT
BsaWI 1499 TCCGGA [AT]^CCGG[AT]
BseAI 1499 TCCGGA T^CCGGA
BseRI 1431 GAGGAG GAGGAG(10/8)
BspEI 1499 TCCGGA T^CCGGA
BsrBI 253 GAGCGG CCGCTC(-3/-3)
BsrFI 1355 ACCGGC [GA]^CCGG[CT]
BssHII 449 GCGCGC G^CGCGC
BssSI 601 CACGAG CACGAG(-5/-1)
BstEII 1374 GGTGACC G^GT[ACGT]ACC
BstPI 1374 GGTGACC G^GT[ACGT]ACC
Bsu36I 2054 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1355 ACCGGC [GA]^CCGG[CT]
DraIII 1159 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1971 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 638 AGCGCT AGC^GCT
Eco81I 2054 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1374 GGTGACC G^GT[ACGT]ACC
EcoRI 2323 GAATTC G^AATTC
EcoT22I 1862 ATGCAT ATGCA^T
FbaI 2117 TGATCA T^GATCA
HincII 1615 GTTAAC GT[CT]^[GA]AC
HindII 1615 GTTAAC GT[CT]^[GA]AC
HpaI 1615 GTTAAC GTT^AAC
KasI 5 GGCGCC G^GCGCC
KspI 564 CCGCGG CCGC^GG
MaeI 1210 CTAG C^TAG
MroI 1499 TCCGGA T^CCGGA
NarI 5 GGCGCC GG^CGCC
NcoI 2389 CCATGG C^CATGG
NsiI 1862 ATGCAT ATGCA^T
NspI 1282 GCATGC [GA]CATG^[CT]
PaeR7I 15 CTCGAG C^TCGAG
PflFI 939 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1068 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1932 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 307 GAGCTC GAGCT^C
SacII 564 CCGCGG CCGC^GG
SapI 2286 GAAGAGC GCTCTTC(1/4)
SexAI 1223 ACCAGGT A^CC[AT]GGT
SfoI 5 GGCGCC GGC^GCC
SmlI 15 CTCGAG C^T[CT][GA]AG
SphI 1282 GCATGC GCATG^C
SspI 1729 AATATT AAT^ATT
Tth111I 939 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1068 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 15 CTCGAG C^TCGAG
XmnI 2478 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflIII 1121 ACGTGT A^C[GA][CT]GT
1487 ACACGT
ApaI 441 GGGCCC GGGCC^C
1445 GGGCCC
BamHI 1502 GGATCC G^GATCC
2160 GGATCC
BanI 5 GGCGCC G^G[CT][GA]CC
1134 GGCACC
BbrPI 436 CACGTG CAC^GTG
1120 CACGTG
BcgI 17 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1383 CGAGCTGCTTGC
BsaAI 436 CACGTG [CT]AC^GT[GA]
1120 CACGTG
BsaI 1938 GGTCTC GGTCTC(1/5)
1979 GGTCTC
BsmI 1236 GAATGC GAATGC(1/-1)
2082 GCATTC
BspMI 408 GCAGGT ACCTGC(4/8)
608 GCAGGT
BstXI 1271 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2355 CCAGGACTTTGG
EaeI 422 TGGCCG [CT]^GGCC[GA]
572 CGGCCA
EarI 1302 GAAGAG CTCTTC(1/4)
2286 GAAGAG
Ksp632I 1302 GAAGAG CTCTTC(1/4)
2286 GAAGAG
PmaCI 436 CACGTG CAC^GTG
1120 CACGTG
PmlI 436 CACGTG CAC^GTG
1120 CACGTG
PpuMI 1247 AGGTCCC [GA]G^G[AT]CC[CT]
2058 AGGTCCT
PstI 259 CTGCAG CTGCA^G
2556 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaLI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI