Restriction map for
pF1KSDA1561
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# NO CUT ENZYMES ( 100 enzymes) #
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Acc65I AccIII AclI AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BlnI BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrFI BsrGI BstBI
BstEII BstPI BstXI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI KpnI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspBI StuI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 150 GACGTC GACGT^C
Asp700I 4192 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 58 GGCGCC G^G[CT][GA]CC
BbeI 58 GGCGCC GGCGC^C
BclI 1134 TGATCA T^GATCA
BcnI 40 CCCGG CC^[GC]GG
BglI 421 GCCGCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2035 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2035 GCTCAGC GC^T[ACGT]AGC
BsaBI 2674 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3162 TCATGA T^CATGA
BspLU11I 3330 ACATGT A^CATGT
BspMI 392 ACCTGC ACCTGC(4/8)
BsrBI 569 GAGCGG CCGCTC(-3/-3)
BssHII 74 GCGCGC G^CGCGC
BssSI 2782 CACGAG CACGAG(-5/-1)
Bst1107I 1235 GTATAC GTA^TAC
BstAPI 30 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1235 GTATAC GTA^TAC
CelII 2035 GCTCAGC GC^T[ACGT]AGC
DraII 469 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 465 CCATGG C^C[GA][CT]GG
EcoO109I 469 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 465 CCATGG C^C[AT][AT]GG
FbaI 1134 TGATCA T^GATCA
HaeII 58 GGCGCC [GA]GCGC^[CT]
HapII 41 CCGG C^CGG
HgaI 49 GCGTC GACGC(5/10)
HpaI 2121 GTTAAC GTT^AAC
HpaII 41 CCGG C^CGG
KasI 58 GGCGCC G^GCGCC
MamI 2674 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 41 CCGG C^CGG
NarI 58 GGCGCC GG^CGCC
NciI 40 CCCGG CC^[GC]GG
NcoI 465 CCATGG C^CATGG
NdeI 1246 CATATG CA^TATG
PacI 655 TTAATTAA TTAAT^TAA
RcaI 3162 TCATGA T^CATGA
SbfI 393 CCTGCAGG CCTGCA^GG
SfoI 58 GGCGCC GGC^GCC
Sse8387I 393 CCTGCAGG CCTGCA^GG
StyI 465 CCATGG C^C[AT][AT]GG
SwaI 1116 ATTTAAAT ATTT^AAAT
TaqI 2921 TCGA T^CGA
TthHB8I 2921 TCGA T^CGA
XmnI 4192 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 493 GTAGAC GT^[AC][GT]AC
1235 GTATAC
AccII 48 CGCG CG^CG
75 CGCG
AcyI 58 GGCGCC G[GA]^CG[CT]C
150 GACGTC
AflIII 2621 ACACGT A^C[GA][CT]GT
3330 ACATGT
AocI 21 CCTGAGG CC^T[ACGT]AGG
1595 CCTCAGG
BglII 205 AGATCT A^GATCT
1258 AGATCT
BpmI 4213 CTCCAG CTGGAG(16/14)
4096 CTGGAG
BsaHI 58 GGCGCC G[GA]^CG[CT]C
150 GACGTC
BsrDI 424 GCAATG GCAATG(2/0)
1342 GCAATG
BstUI 48 CGCG CG^CG
75 CGCG
BstYI 205 AGATCT [GA]^GATC[CT]
1258 AGATCT
Bsu36I 21 CCTGAGG CC^T[ACGT]AGG
1595 CCTCAGG
Eco81I 21 CCTGAGG CC^T[ACGT]AGG
1595 CCTCAGG
FauI 17 CCCGC CCCGC(4/6)
46 CCCGC
Hin1I 58 GGCGCC G[GA]^CG[CT]C
150 GACGTC
MflI 205 AGATCT [GA]^GATC[CT]
1258 AGATCT
MvnI 48 CGCG CG^CG
75 CGCG
XhoII 205 AGATCT [GA]^GATC[CT]
1258 AGATCT
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AspHI BalI BanII
BbsI BbvI BfaI BfrI BmyI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraIII EaeI EarI EcoRII
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MluNI
MnlI MscI MseI MslI MspA1I
MunI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI