Restriction map for
pF1KSDA1710
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AlwNI 362 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 71 CCCGGG C^[CT]CG[GA]G
BalI 56 TGGCCA TGG^CCA
BanI 33 GGCACC G^G[CT][GA]CC
BbsI 471 GAAGAC GAAGAC(2/6)
BbvCI 256 GCTGAGG CCTCAGC(-5/-2)
BpuAI 471 GAAGAC GAAGAC(2/6)
BsaI 84 GAGACC GGTCTC(1/5)
BseRI 757 GAGGAG GAGGAG(10/8)
BsmBI 697 CGTCTC CGTCTC(1/5)
BsoBI 71 CCCGGG C^[CT]CG[GA]G
Bsp1407I 216 TGTACA T^GTACA
BspMI 243 ACCTGC ACCTGC(4/8)
BsrDI 1091 GCAATG GCAATG(2/0)
BsrGI 216 TGTACA T^GTACA
BstNI 30 CCAGG CC^[AT]GG
EcoRII 30 CCAGG ^CC[AT]GG
MaeII 43 ACGT A^CGT
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MvaI 30 CCAGG CC^[AT]GG
NdeI 1082 CATATG CA^TATG
PleI 893 GAGTC GAGTC(4/5)
SmaI 71 CCCGGG CCC^GGG
SspBI 216 TGTACA T^GTACA
TfiI 627 GATTC G^A[AT]TC
XcmI 8 CCACCCTGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 1023 AAATTT [GA]^AATT[CT]
1145 AAATTC
AfaI 217 GTAC GT^AC
1343 GTAC
ApoI 1023 AAATTT [GA]^AATT[CT]
1145 AAATTC
BfaI 157 CTAG C^TAG
793 CTAG
BlpI 796 GCTCAGC GC^T[ACGT]AGC
1373 GCTCAGC
Bpu1102I 796 GCTCAGC GC^T[ACGT]AGC
1373 GCTCAGC
BsgI 98 CTGCAC GTGCAG(16/14)
1261 GTGCAG
BsmI 252 GAATGC GAATGC(1/-1)
1087 GAATGC
BstF5I 402 CATCC GGATG(2/0)
120 GGATG
CelII 796 GCTCAGC GC^T[ACGT]AGC
1373 GCTCAGC
EaeI 56 TGGCCA [CT]^GGCC[GA]
849 TGGCCG
FokI 402 CATCC GGATG(9/13)
120 GGATG
HgaI 94 GACGC GACGC(5/10)
966 GCGTC
HinfI 627 GATTC G^A[ACGT]TC
893 GAGTC
MaeI 157 CTAG C^TAG
793 CTAG
NlaIII 18 CATG CATG^
60 CATG
RsaI 217 GTAC GT^AC
1343 GTAC
StuI 434 AGGCCT AGG^CCT
574 AGGCCT
TaqI 462 TCGA T^CGA
891 TCGA
Tsp45I 40 GTGAC ^GT[GC]AC
364 GTCAC
TthHB8I 462 TCGA T^CGA
891 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI Asp700I AspHI AvaII
BanII BbvI BcnI BglII BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstYI Cfr13I DdeI DpnII DraII
EarI EcoO109I Fnu4HI HaeIII HapII
HindIII HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MwoI NciI
NdeII NlaIV PpuMI SacI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp509I TspRI XhoII XmnI