Restriction map for
pF1KSDA1932
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I AspI AviII AvrII
BamHI BbrPI BciVI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MroI MslI
MunI NcoI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 266 GGTACC G^GTACC
AhdI 579 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 206 GTGCAC G^TGCAC
AocI 1267 CCTAAGG CC^T[ACGT]AGG
Aor51HI 782 AGCGCT AGC^GCT
ApaLI 206 GTGCAC G^TGCAC
Asp718I 266 GGTACC G^GTACC
AspEI 579 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 206 GTGCAC G[AT]GC[AT]^C
BbeI 1344 GGCGCC GGCGC^C
BlpI 379 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 379 GCTCAGC GC^T[ACGT]AGC
BsaAI 214 TACGTG [CT]AC^GT[GA]
BsgI 1723 GTGCAG GTGCAG(16/14)
BsiHKAI 206 GTGCAC G[AT]GC[AT]^C
BsmI 2278 GCATTC GAATGC(1/-1)
BspHI 1109 TCATGA T^CATGA
BstEII 1792 GGTGACC G^GT[ACGT]ACC
BstPI 1792 GGTGACC G^GT[ACGT]ACC
Bsu36I 1267 CCTAAGG CC^T[ACGT]AGG
CelII 379 GCTCAGC GC^T[ACGT]AGC
DraIII 1240 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 579 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 782 AGCGCT AGC^GCT
Eco81I 1267 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1792 GGTGACC G^GT[ACGT]ACC
KasI 1344 GGCGCC G^GCGCC
KpnI 266 GGTACC GGTAC^C
MluI 1676 ACGCGT A^CGCGT
NaeI 163 GCCGGC GCC^GGC
NarI 1344 GGCGCC GG^CGCC
NdeI 1294 CATATG CA^TATG
NgoMIV 163 GCCGGC G^CCGGC
NheI 967 GCTAGC G^CTAGC
PaeR7I 1465 CTCGAG C^TCGAG
RcaI 1109 TCATGA T^CATGA
ScaI 254 AGTACT AGT^ACT
SfiI 123 GGCCCGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1344 GGCGCC GGC^GCC
StuI 494 AGGCCT AGG^CCT
TfiI 1886 GAATC G^A[AT]TC
XhoI 1465 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 215 ACGTGT A^C[GA][CT]GT
1676 ACGCGT
BalI 137 TGGCCA TGG^CCA
200 TGGCCA
BbsI 39 GTCTTC GAAGAC(2/6)
1105 GTCTTC
BcgI 1761 CGAGACCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1869 CGAGTTTACTGC
BclI 18 TGATCA T^GATCA
2120 TGATCA
BfaI 968 CTAG C^TAG
986 CTAG
BglI 124 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2107 GCCCTCAAGGC
BpuAI 39 GTCTTC GAAGAC(2/6)
1105 GTCTTC
BsaI 1762 GAGACC GGTCTC(1/5)
586 GGTCTC
BsiEI 1059 CGGTCG CG[GA][CT]^CG
1281 CGGTCG
BsmBI 2181 CGTCTC CGTCTC(1/5)
88 GAGACG
BsrBI 1146 CCGCTC CCGCTC(-3/-3)
1152 CCGCTC
BsrDI 188 GCAATG GCAATG(2/0)
1859 GCAATG
BsrFI 163 GCCGGC [GA]^CCGG[CT]
1057 GCCGGT
BstAPI 1524 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1566 GCACTACCTGC
BstYI 1713 GGATCT [GA]^GATC[CT]
1965 AGATCC
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
1057 GCCGGT
DsaI 485 CCACGG C^C[GA][CT]GG
1928 CCACGG
EcoNI 982 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1157 CCTCCTCCAGG
FbaI 18 TGATCA T^GATCA
2120 TGATCA
MaeI 968 CTAG C^TAG
986 CTAG
MflI 1713 GGATCT [GA]^GATC[CT]
1965 AGATCC
MluNI 137 TGGCCA TGG^CCA
200 TGGCCA
MscI 137 TGGCCA TGG^CCA
200 TGGCCA
MseI 1808 TTAA T^TAA
1993 TTAA
NspI 341 GCATGT [GA]CATG^[CT]
2057 GCATGT
PflMI 884 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCACGCCTTGG
PvuII 367 CAGCTG CAG^CTG
547 CAGCTG
SapI 1124 GCTCTTC GCTCTTC(1/4)
2160 GCTCTTC
Tru9I 1808 TTAA T^TAA
1993 TTAA
Van91I 884 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCACGCCTTGG
XhoII 1713 GGATCT [GA]^GATC[CT]
1965 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI