Restriction map for
pF1KB7814
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbsI BbvCI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsiWI
BspDI BspHI BsrDI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1323 GACGTC GACGT^C
AccIII 185 TCCGGA T^CCGGA
Alw44I 700 GTGCAC G^TGCAC
AlwNI 533 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 728 GGGCCC GGGCC^C
ApaLI 700 GTGCAC G^TGCAC
AspI 1339 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 614 CTCGAG C^[CT]CG[GA]G
BalI 1466 TGGCCA TGG^CCA
BanII 728 GGGCCC G[GA]GC[CT]^C
BbeI 1459 GGCGCC GGCGC^C
BbrPI 670 CACGTG CAC^GTG
BcgI 1558 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 115 GCCAACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 372 AGATCT A^GATCT
BpmI 334 CTCCAG CTGGAG(16/14)
BsaAI 670 CACGTG [CT]AC^GT[GA]
BsaWI 185 TCCGGA [AT]^CCGG[AT]
BseAI 185 TCCGGA T^CCGGA
BsiEI 122 CGGCCG CG[GA][CT]^CG
BsmAI 1321 GAGAC GTCTC(1/5)
BsmBI 1321 GAGACG CGTCTC(1/5)
BsoBI 614 CTCGAG C^[CT]CG[GA]G
BspEI 185 TCCGGA T^CCGGA
BspLU11I 692 ACATGT A^CATGT
BspMI 1620 ACCTGC ACCTGC(4/8)
BstAPI 482 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 531 CTCAG C^T[ACGT]AG
DraII 1478 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 122 CGGCCG C^GGCCG
EclXI 122 CGGCCG C^GGCCG
Eco52I 122 CGGCCG C^GGCCG
EcoO109I 1478 GGGTCCC [GA]G^G[ACGT]CC[CT]
KasI 1459 GGCGCC G^GCGCC
MluNI 1466 TGGCCA TGG^CCA
MroI 185 TCCGGA T^CCGGA
MscI 1466 TGGCCA TGG^CCA
NarI 1459 GGCGCC GG^CGCC
NheI 1302 GCTAGC G^CTAGC
NspI 692 ACATGT [GA]CATG^[CT]
PaeR7I 614 CTCGAG C^TCGAG
PflFI 1339 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 669 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 670 CACGTG CAC^GTG
PmlI 670 CACGTG CAC^GTG
PpuMI 1478 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 648 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1650 GAAGAGC GCTCTTC(1/4)
ScaI 1399 AGTACT AGT^ACT
SexAI 1127 ACCTGGT A^CC[AT]GGT
SfoI 1459 GGCGCC GGC^GCC
SspI 19 AATATT AAT^ATT
Tth111I 1339 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 669 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1544 CCAGCCCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 614 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 418 GTCTAC GT^[AC][GT]AC
1003 GTCTAC
AflIII 692 ACATGT A^C[GA][CT]GT
1313 ACGTGT
AspHI 700 GTGCAC G[AT]GC[AT]^C
986 GTGCTC
BanI 805 GGTGCC G^G[CT][GA]CC
1459 GGCGCC
BsiHKAI 700 GTGCAC G[AT]GC[AT]^C
986 GTGCTC
BsmI 600 GAATGC GAATGC(1/-1)
1148 GAATGC
Bsp1407I 344 TGTACA T^GTACA
1208 TGTACA
BsrBI 132 GAGCGG CCGCTC(-3/-3)
1184 GAGCGG
BsrFI 240 GCCGGT [GA]^CCGG[CT]
485 GCCGGT
BsrGI 344 TGTACA T^GTACA
1208 TGTACA
BssSI 469 CACGAG CACGAG(-5/-1)
84 CTCGTG
BstEII 1091 GGTTACC G^GT[ACGT]ACC
1569 GGTCACC
BstPI 1091 GGTTACC G^GT[ACGT]ACC
1569 GGTCACC
BstYI 372 AGATCT [GA]^GATC[CT]
1235 AGATCC
Cfr10I 240 GCCGGT [GA]^CCGG[CT]
485 GCCGGT
DraIII 205 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
756 CACTCCGTG
EarI 1563 CTCTTC CTCTTC(1/4)
1650 GAAGAG
EcoO65I 1091 GGTTACC G^GT[ACGT]ACC
1569 GGTCACC
Ksp632I 1563 CTCTTC CTCTTC(1/4)
1650 GAAGAG
MflI 372 AGATCT [GA]^GATC[CT]
1235 AGATCC
MseI 1016 TTAA T^TAA
1502 TTAA
MspA1I 55 CCGCTG C[AC]G^C[GT]G
63 CAGCGG
NcoI 1463 CCATGG C^CATGG
1553 CCATGG
PleI 356 GAGTC GAGTC(4/5)
908 GAGTC
SfcI 1350 CTACAG C^T[GA][CT]AG
1587 CTGTAG
SmlI 574 CTCAAG C^T[CT][GA]AG
614 CTCGAG
SspBI 344 TGTACA T^GTACA
1208 TGTACA
Tru9I 1016 TTAA T^TAA
1502 TTAA
TspRI 783 CAGTG CA[GC]TG[ACGT][ACGT]^
1640 CAGTG
XhoII 372 AGATCT [GA]^GATC[CT]
1235 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI Tsp45I Tsp509I TthHB8I