Restriction map for
pF1KSDF0128
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI AviII AvrII BbeI BbsI
BfrI BlnI BpuAI BsaBI BsiEI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco52I EcoRI
EcoT22I FseI FspI HincII HindII
HpaI KasI KspI MamI MfeI
MluI MunI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 4366 GACGTC GACGT^C
AccI 3142 GTAGAC GT^[AC][GT]AC
AccII 4339 CGCG CG^CG
AcsI 4376 AAATTT [GA]^AATT[CT]
AflIII 3168 ACACGT A^C[GA][CT]GT
AhdI 2434 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4376 AAATTT [GA]^AATT[CT]
Asp700I 3391 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2434 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4514 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2852 GGATCC G^GATCC
BcgI 3850 CGAAAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 51 GTATCC GTATCC(6/5)
BclI 1563 TGATCA T^GATCA
BglII 3792 AGATCT A^GATCT
BlpI 535 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 535 GCTGAGC GC^T[ACGT]AGC
BsmBI 1557 CGTCTC CGTCTC(1/5)
Bsp1407I 3717 TGTACA T^GTACA
BspHI 1881 TCATGA T^CATGA
BsrDI 3016 CATTGC GCAATG(2/0)
BsrGI 3717 TGTACA T^GTACA
BssSI 4037 CACGAG CACGAG(-5/-1)
BstEII 3123 GGTCACC G^GT[ACGT]ACC
BstPI 3123 GGTCACC G^GT[ACGT]ACC
BstUI 4339 CGCG CG^CG
CelII 535 GCTGAGC GC^T[ACGT]AGC
DraIII 1256 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2434 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3123 GGTCACC G^GT[ACGT]ACC
EcoRV 2563 GATATC GAT^ATC
FbaI 1563 TGATCA T^GATCA
MvnI 4339 CGCG CG^CG
NheI 273 GCTAGC G^CTAGC
NspI 4284 GCATGC [GA]CATG^[CT]
PaeR7I 4526 CTCGAG C^TCGAG
PflFI 4514 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1777 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1881 TCATGA T^CATGA
SapI 4238 GAAGAGC GCTCTTC(1/4)
SbfI 2448 CCTGCAGG CCTGCA^GG
SphI 4284 GCATGC GCATG^C
Sse8387I 2448 CCTGCAGG CCTGCA^GG
SspBI 3717 TGTACA T^GTACA
Tsp509I 4377 AATT ^AATT
Tth111I 4514 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4526 CTCGAG C^TCGAG
XmnI 3391 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 803 TCCGGA T^CCGGA
1033 TCCGGA
AcyI 3345 GACGCC G[GA]^CG[CT]C
4366 GACGTC
Alw44I 559 GTGCAC G^TGCAC
4034 GTGCAC
Aor51HI 2482 AGCGCT AGC^GCT
2624 AGCGCT
ApaLI 559 GTGCAC G^TGCAC
4034 GTGCAC
BbrPI 2326 CACGTG CAC^GTG
3169 CACGTG
BsaHI 3345 GACGCC G[GA]^CG[CT]C
4366 GACGTC
BsaI 3752 GAGACC GGTCTC(1/5)
3824 GAGACC
BseAI 803 TCCGGA T^CCGGA
1033 TCCGGA
BspEI 803 TCCGGA T^CCGGA
1033 TCCGGA
BsrBI 241 CCGCTC CCGCTC(-3/-3)
4210 GAGCGG
BstXI 77 CCACCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCAATACAGTGG
Eco47III 2482 AGCGCT AGC^GCT
2624 AGCGCT
Hin1I 3345 GACGCC G[GA]^CG[CT]C
4366 GACGTC
HindIII 1270 AAGCTT A^AGCTT
2415 AAGCTT
MroI 803 TCCGGA T^CCGGA
1033 TCCGGA
MseI 1292 TTAA T^TAA
3956 TTAA
NaeI 2746 GCCGGC GCC^GGC
4288 GCCGGC
NcoI 3578 CCATGG C^CATGG
4163 CCATGG
NgoMIV 2746 GCCGGC G^CCGGC
4288 GCCGGC
PmaCI 2326 CACGTG CAC^GTG
3169 CACGTG
PmlI 2326 CACGTG CAC^GTG
3169 CACGTG
PpuMI 708 AGGACCT [GA]G^G[AT]CC[CT]
1997 GGGACCC
SexAI 1104 ACCTGGT A^CC[AT]GGT
1286 ACCTGGT
SfiI 1459 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4257 GGCCTCCGTGGCC
SmaI 3386 CCCGGG CCC^GGG
4253 CCCGGG
Tru9I 1292 TTAA T^TAA
3956 TTAA
XmaCI 3386 CCCGGG C^CCGGG
4253 CCCGGG
XmaI 3386 CCCGGG C^CCGGG
4253 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII