Restriction map for
pF1KF0405
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 367 GTCTAC GT^[AC][GT]AC
AciI 572 GCGG CCGC(-3/-1)
AclI 95 AACGTT AA^CGTT
AcsI 720 AAATTC [GA]^AATT[CT]
Alw44I 376 GTGCAC G^TGCAC
AlwNI 613 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 376 GTGCAC G^TGCAC
ApoI 720 AAATTC [GA]^AATT[CT]
Asp700I 604 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 376 GTGCAC G[AT]GC[AT]^C
AvaII 866 GGTCC G^G[AT]CC
BanII 6 GGGCTC G[GA]GC[CT]^C
BcgI 347 CGATTTATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1138 CTAG C^TAG
BglI 793 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 413 GTGCAG GTGCAG(16/14)
BsiHKAI 376 GTGCAC G[AT]GC[AT]^C
BsiYI 1122 CCTTACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1122 CCTTACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 35 CGTCTC CGTCTC(1/5)
BsmFI 812 GGGAC GGGAC(10/14)
BsmI 1132 GAATGC GAATGC(1/-1)
Bsp1407I 1026 TGTACA T^GTACA
BspHI 765 TCATGA T^CATGA
BsrGI 1026 TGTACA T^GTACA
BsrI 958 ACTGG ACTGG(1/-1)
BstBI 236 TTCGAA TT^CGAA
BstXI 669 CCACGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 GGATCT [GA]^GATC[CT]
CfoI 32 GCGC GCG^C
Cfr13I 866 GGTCC G^G[ACGT]CC
DsaI 1014 CCACGG C^C[GA][CT]GG
EaeI 1017 CGGCCA [CT]^GGCC[GA]
EcoT14I 869 CCAAGG C^C[AT][AT]GG
FauI 572 GCGGG CCCGC(4/6)
HgaI 34 GCGTC GACGC(5/10)
HhaI 32 GCGC GCG^C
HinP1I 32 GCGC G^CGC
MaeI 1138 CTAG C^TAG
MflI 488 GGATCT [GA]^GATC[CT]
NruI 1152 TCGCGA TCG^CGA
NspV 236 TTCGAA TT^CGAA
PleI 469 GAGTC GAGTC(4/5)
Psp1406I 95 AACGTT AA^CGTT
RcaI 765 TCATGA T^CATGA
SapI 336 GCTCTTC GCTCTTC(1/4)
Sau96I 866 GGTCC G^G[ACGT]CC
SfcI 879 CTACAG C^T[GA][CT]AG
SfuI 236 TTCGAA TT^CGAA
SspBI 1026 TGTACA T^GTACA
StyI 869 CCAAGG C^C[AT][AT]GG
TaqI 237 TCGA T^CGA
TfiI 918 GATTC G^A[AT]TC
TthHB8I 237 TCGA T^CGA
XhoII 488 GGATCT [GA]^GATC[CT]
XmnI 604 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 33 CGCG CG^CG
1153 CGCG
AfaI 228 GTAC GT^AC
1027 GTAC
AlwI 384 GGATC GGATC(4/5)
488 GGATC
BbsI 523 GAAGAC GAAGAC(2/6)
994 GAAGAC
BcnI 649 CCCGG CC^[GC]GG
922 CCCGG
BmyI 6 GGGCTC G[AGT]GC[ACT]^C
376 GTGCAC
BpmI 492 CTCCAG CTGGAG(16/14)
499 CTCCAG
BpuAI 523 GAAGAC GAAGAC(2/6)
994 GAAGAC
BsaAI 727 TACGTG [CT]AC^GT[GA]
796 TACGTG
BsmAI 1055 GAGAC GTCTC(1/5)
36 GTCTC
Bsp1286I 6 GGGCTC G[AGT]GC[ACT]^C
376 GTGCAC
BspMI 217 ACCTGC ACCTGC(4/8)
568 ACCTGC
BstUI 33 CGCG CG^CG
1153 CGCG
Cac8I 572 GCGGGC GC[ACGT]^[ACGT]GC
843 GCTCGC
DpnII 385 GATC ^GATC
489 GATC
DraI 406 TTTAAA TTT^AAA
628 TTTAAA
EarI 337 CTCTTC CTCTTC(1/4)
271 GAAGAG
EcoT22I 188 ATGCAT ATGCA^T
947 ATGCAT
HapII 650 CCGG C^CGG
923 CCGG
HinfI 469 GAGTC G^A[ACGT]TC
918 GATTC
HpaII 650 CCGG C^CGG
923 CCGG
Ksp632I 337 CTCTTC CTCTTC(1/4)
271 GAAGAG
MboI 385 GATC ^GATC
489 GATC
MspA1I 432 CAGCTG C[AC]G^C[GT]G
1194 CAGCTG
MspI 650 CCGG C^CGG
923 CCGG
MvnI 33 CGCG CG^CG
1153 CGCG
NciI 649 CCCGG CC^[GC]GG
922 CCCGG
NdeII 385 GATC ^GATC
489 GATC
NlaIV 281 GGTTCC GG[ACGT]^[ACGT]CC
497 GGCTCC
NsiI 188 ATGCAT ATGCA^T
947 ATGCAT
PvuII 432 CAGCTG CAG^CTG
1194 CAGCTG
RsaI 228 GTAC GT^AC
1027 GTAC
Sau3AI 385 GATC ^GATC
489 GATC
XcmI 943 CCAAATGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1020 CCACTATGTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BsaJI BseRI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeII
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI