Restriction map for
pF1KA0038
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BseRI BsgI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI MamI MfeI MluI MroI
MslI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 597 GACGCC G[GA]^CG[CT]C
AfaI 208 GTAC GT^AC
AflII 283 CTTAAG C^TTAAG
Alw44I 308 GTGCAC G^TGCAC
ApaLI 308 GTGCAC G^TGCAC
Asp700I 661 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 78 TGGCCA TGG^CCA
BamHI 514 GGATCC G^GATCC
BanI 643 GGTGCC G^G[CT][GA]CC
BanII 115 GAGCCC G[GA]GC[CT]^C
BcgI 168 CGACATAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 283 CTTAAG C^TTAAG
BpmI 571 CTCCAG CTGGAG(16/14)
BsaAI 133 TACGTA [CT]AC^GT[GA]
BsaHI 597 GACGCC G[GA]^CG[CT]C
BsaI 561 GAGACC GGTCTC(1/5)
BsmFI 60 GTCCC GGGAC(10/14)
BsrDI 342 CATTGC GCAATG(2/0)
BsrFI 458 ACCGGC [GA]^CCGG[CT]
BstF5I 417 GGATG GGATG(2/0)
Cfr10I 458 ACCGGC [GA]^CCGG[CT]
DraI 241 TTTAAA TTT^AAA
DraIII 329 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 78 TGGCCA [CT]^GGCC[GA]
EarI 544 GAAGAG CTCTTC(1/4)
EcoRI 262 GAATTC G^AATTC
FauI 62 CCCGC CCCGC(4/6)
FokI 417 GGATG GGATG(9/13)
HapII 459 CCGG C^CGG
HgaI 597 GACGC GACGC(5/10)
Hin1I 597 GACGCC G[GA]^CG[CT]C
HpaII 459 CCGG C^CGG
Ksp632I 544 GAAGAG CTCTTC(1/4)
KspI 63 CCGCGG CCGC^GG
MaeII 134 ACGT A^CGT
MaeIII 327 GTCAC ^GT[ACGT]AC
MluNI 78 TGGCCA TGG^CCA
MscI 78 TGGCCA TGG^CCA
MspA1I 63 CCGCGG C[AC]G^C[GT]G
MspI 459 CCGG C^CGG
NruI 593 TCGCGA TCG^CGA
PleI 569 GACTC GAGTC(4/5)
PpuMI 58 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 208 GTAC GT^AC
SacII 63 CCGCGG CCGC^GG
SfaNI 175 GATGC GCATC(5/9)
SfcI 33 CTACAG C^T[GA][CT]AG
SmlI 283 CTTAAG C^T[CT][GA]AG
SnaBI 133 TACGTA TAC^GTA
StuI 649 AGGCCT AGG^CCT
Tsp45I 327 GTCAC ^GT[GC]AC
XmnI 661 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 64 CGCG CG^CG
594 CGCG
AcsI 238 AAATTT [GA]^AATT[CT]
262 GAATTC
AluI 40 AGCT AG^CT
575 AGCT
ApoI 238 AAATTT [GA]^AATT[CT]
262 GAATTC
AspHI 308 GTGCAC G[AT]GC[AT]^C
554 GAGCAC
AvaII 59 GGTCC G^G[AT]CC
394 GGACC
BbvI 38 GCAGC GCAGC(8/12)
482 GCAGC
BfaI 214 CTAG C^TAG
653 CTAG
BsiHKAI 308 GTGCAC G[AT]GC[AT]^C
554 GAGCAC
BsmAI 221 GAGAC GTCTC(1/5)
561 GAGAC
BssKI 451 CCAGG ^CC[ACGT]GG
647 CCAGG
BstNI 451 CCAGG CC^[AT]GG
647 CCAGG
BstUI 64 CGCG CG^CG
594 CGCG
BstYI 189 GGATCT [GA]^GATC[CT]
514 GGATCC
DdeI 193 CTCAG C^T[ACGT]AG
426 CTTAG
DraII 58 GGGTCCC [GA]G^G[ACGT]CC[CT]
468 AGGCCCC
EcoO109I 58 GGGTCCC [GA]G^G[ACGT]CC[CT]
468 AGGCCCC
EcoRII 451 CCAGG ^CC[AT]GG
647 CCAGG
EcoT14I 81 CCATGG C^C[AT][AT]GG
476 CCATGG
MaeI 214 CTAG C^TAG
653 CTAG
MboII 544 GAAGA GAAGA(8/7)
635 TCTTC
MflI 189 GGATCT [GA]^GATC[CT]
514 GGATCC
MvaI 451 CCAGG CC^[AT]GG
647 CCAGG
MvnI 64 CGCG CG^CG
594 CGCG
NcoI 81 CCATGG C^CATGG
476 CCATGG
PvuI 24 CGATCG CGAT^CG
321 CGATCG
ScrFI 451 CCAGG CC^[ACGT]GG
647 CCAGG
StyI 81 CCATGG C^C[AT][AT]GG
476 CCATGG
TseI 38 GCAGC G^C[AT]GC
482 GCAGC
Tsp509I 239 AATT ^AATT
263 AATT
TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^
311 CACTG
XhoII 189 GGATCT [GA]^GATC[CT]
514 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AlwI BmyI BsaJI BsiEI
BsiYI BslI Bsp1286I Cfr13I DpnII
DsaI Fnu4HI HaeIII HinfI ItaI
MboI MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I TaqI
TfiI Tru9I TthHB8I