################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BlnI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrBI BssHII BssSI BstEII BstPI BstUI Bsu36I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MluI MroI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SapI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AspHI 2911 GAGCAC G[AT]GC[AT]^C AvaII 238 GGTCC G^G[AT]CC BalI 3116 TGGCCA TGG^CCA BciVI 2460 GGATAC GTATCC(6/5) BclI 204 TGATCA T^GATCA BglI 3958 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1464 AGATCT A^GATCT BlpI 1552 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1552 GCTGAGC GC^T[ACGT]AGC BsaAI 3614 TACGTG [CT]AC^GT[GA] BsaBI 1459 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 4030 GTGCAG GTGCAG(16/14) BsiHKAI 2911 GAGCAC G[AT]GC[AT]^C BspHI 3780 TCATGA T^CATGA BspLU11I 4203 ACATGT A^CATGT BspMI 2139 ACCTGC ACCTGC(4/8) BsrFI 3020 GCCGGC [GA]^CCGG[CT] Bst1107I 403 GTATAC GTA^TAC BstBI 3938 TTCGAA TT^CGAA BstXI 1962 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 403 GTATAC GTA^TAC CelII 1552 GCTGAGC GC^T[ACGT]AGC Cfr10I 3020 GCCGGC [GA]^CCGG[CT] DraII 1528 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoNI 2478 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1528 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 94 GAATTC G^AATTC EcoRV 2998 GATATC GAT^ATC FbaI 204 TGATCA T^GATCA HapII 3021 CCGG C^CGG HpaII 3021 CCGG C^CGG MamI 1459 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3116 TGGCCA TGG^CCA MscI 3116 TGGCCA TGG^CCA MspI 3021 CCGG C^CGG NaeI 3020 GCCGGC GCC^GGC NgoMIV 3020 GCCGGC G^CCGGC NspV 3938 TTCGAA TT^CGAA PshAI 3844 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3780 TCATGA T^CATGA SalI 3035 GTCGAC G^TCGAC SbfI 4007 CCTGCAGG CCTGCA^GG ScaI 2074 AGTACT AGT^ACT SexAI 4269 ACCTGGT A^CC[AT]GGT SfuI 3938 TTCGAA TT^CGAA Sse8387I 4007 CCTGCAGG CCTGCA^GG StuI 1252 AGGCCT AGG^CCT XbaI 1029 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 1515 GACGTC GACGT^C 2549 GACGTC AcyI 1515 GACGTC G[GA]^CG[CT]C 2549 GACGTC AvaI 79 CTCGAG C^[CT]CG[GA]G 4217 CTCGAG BanII 1555 GAGCCC G[GA]GC[CT]^C 2443 GAGCCC BsaHI 1515 GACGTC G[GA]^CG[CT]C 2549 GACGTC BsaI 410 GAGACC GGTCTC(1/5) 2382 GGTCTC BsoBI 79 CTCGAG C^[CT]CG[GA]G 4217 CTCGAG BsrDI 4404 CATTGC GCAATG(2/0) 4120 GCAATG BstAPI 3463 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4360 GCAGAAGCTGC BstYI 1464 AGATCT [GA]^GATC[CT] 3078 AGATCC CfoI 3005 GCGC GCG^C 3302 GCGC HhaI 3005 GCGC GCG^C 3302 GCGC Hin1I 1515 GACGTC G[GA]^CG[CT]C 2549 GACGTC HinP1I 3005 GCGC G^CGC 3302 GCGC HindIII 217 AAGCTT A^AGCTT 2366 AAGCTT MfeI 694 CAATTG C^AATTG 914 CAATTG MflI 1464 AGATCT [GA]^GATC[CT] 3078 AGATCC MspA1I 3047 CAGCTG C[AC]G^C[GT]G 3968 CAGCTG MunI 694 CAATTG C^AATTG 914 CAATTG PaeR7I 79 CTCGAG C^TCGAG 4217 CTCGAG PvuII 3047 CAGCTG CAG^CTG 3968 CAGCTG SspI 464 AATATT AAT^ATT 946 AATATT XcmI 2984 CCAAAATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3725 CCAACCCCCCTGTGG XhoI 79 CTCGAG C^TCGAG 4217 CTCGAG XhoII 1464 AGATCT [GA]^GATC[CT] 3078 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraIII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI