Restriction map for
pF1KA0407
##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AvrII BclI BfrI
BlnI BsiWI BspDI BspLU11I BstBI
ClaI CpoI DraI Eco47III EcoRV
EcoT22I FbaI HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SfuI SgrAI SpeI
SphI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 6235 TCCGGA T^CCGGA
BamHI 3480 GGATCC G^GATCC
BsaWI 6235 TCCGGA [AT]^CCGG[AT]
BseAI 6235 TCCGGA T^CCGGA
BsiEI 3012 CGGCCG CG[GA][CT]^CG
BspEI 6235 TCCGGA T^CCGGA
BssHII 5727 GCGCGC G^CGCGC
BstEII 3858 GGTGACC G^GT[ACGT]ACC
BstPI 3858 GGTGACC G^GT[ACGT]ACC
EagI 3012 CGGCCG C^GGCCG
EclXI 3012 CGGCCG C^GGCCG
Eco52I 3012 CGGCCG C^GGCCG
EcoO65I 3858 GGTGACC G^GT[ACGT]ACC
EcoRI 4627 GAATTC G^AATTC
HincII 3985 GTCAAC GT[CT]^[GA]AC
HindII 3985 GTCAAC GT[CT]^[GA]AC
HindIII 4942 AAGCTT A^AGCTT
KspI 3510 CCGCGG CCGC^GG
MroI 6235 TCCGGA T^CCGGA
MseI 5141 TTAA T^TAA
PshAI 3861 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3510 CCGCGG CCGC^GG
SbfI 708 CCTGCAGG CCTGCA^GG
SfiI 398 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 1813 TACGTA TAC^GTA
Sse8387I 708 CCTGCAGG CCTGCA^GG
Tru9I 5141 TTAA T^TAA
XbaI 721 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 4060 GAATTT [GA]^AATT[CT]
4627 GAATTC
AhdI 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6170 GACAGACTGTC
ApoI 4060 GAATTT [GA]^AATT[CT]
4627 GAATTC
Asp700I 4623 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5853 GAAGTACTTC
AspEI 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6170 GACAGACTGTC
AviII 1914 TGCGCA TGC^GCA
5027 TGCGCA
BbsI 4281 GAAGAC GAAGAC(2/6)
5928 GAAGAC
BciVI 511 GGATAC GTATCC(6/5)
1816 GTATCC
BglII 5747 AGATCT A^GATCT
6399 AGATCT
BpuAI 4281 GAAGAC GAAGAC(2/6)
5928 GAAGAC
BsaBI 1117 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATGTCCATC
Bsp1407I 4529 TGTACA T^GTACA
5120 TGTACA
BspHI 1403 TCATGA T^CATGA
4640 TCATGA
BsrDI 6033 CATTGC GCAATG(2/0)
4228 GCAATG
BsrGI 4529 TGTACA T^GTACA
5120 TGTACA
Bst1107I 3741 GTATAC GTA^TAC
5085 GTATAC
BstZ17I 3741 GTATAC GTA^TAC
5085 GTATAC
Eam1105I 3446 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6170 GACAGACTGTC
FseI 916 GGCCGGCC GGCCGG^CC
3009 GGCCGGCC
FspI 1914 TGCGCA TGC^GCA
5027 TGCGCA
MamI 1117 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5070 GATGTCCATC
RcaI 1403 TCATGA T^CATGA
4640 TCATGA
SacI 1831 GAGCTC GAGCT^C
2980 GAGCTC
ScaI 5855 AGTACT AGT^ACT
6290 AGTACT
SspBI 4529 TGTACA T^GTACA
5120 TGTACA
XmnI 4623 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5853 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI