Restriction map for
pF1KA0440
##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI BalI BciVI BfrI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI HpaI
KpnI MluI MluNI MroI MscI
NdeI NheI NotI NruI NsiI
PacI PinAI PmeI Psp1406I PvuI
RsrII SbfI SfiI SgrAI SmaI
SnaBI Sse8387I SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 4834 GACGTC GACGT^C
Alw44I 2221 GTGCAC G^TGCAC
ApaLI 2221 GTGCAC G^TGCAC
AviII 896 TGCGCA TGC^GCA
AvrII 4408 CCTAGG C^CTAGG
BamHI 3789 GGATCC G^GATCC
BbeI 3356 GGCGCC GGCGC^C
BbrPI 1726 CACGTG CAC^GTG
BcgI 2967 GCAGGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5139 TGATCA T^GATCA
BlnI 4408 CCTAGG C^CTAGG
BlpI 2556 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2556 GCTCAGC GC^T[ACGT]AGC
BsaBI 1522 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 976 ACATGT A^CATGT
BspMI 5309 ACCTGC ACCTGC(4/8)
BsrFI 4204 GCCGGC [GA]^CCGG[CT]
BssSI 4503 CACGAG CACGAG(-5/-1)
BstBI 1553 TTCGAA TT^CGAA
CelII 2556 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4204 GCCGGC [GA]^CCGG[CT]
DrdI 426 GACAAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 5139 TGATCA T^GATCA
FspI 896 TGCGCA TGC^GCA
HindIII 660 AAGCTT A^AGCTT
KasI 3356 GGCGCC G^GCGCC
KspI 3097 CCGCGG CCGC^GG
MamI 1522 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4204 GCCGGC GCC^GGC
NarI 3356 GGCGCC GG^CGCC
NgoMIV 4204 GCCGGC G^CCGGC
NspV 1553 TTCGAA TT^CGAA
PmaCI 1726 CACGTG CAC^GTG
PmlI 1726 CACGTG CAC^GTG
PshAI 1121 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 5021 CTGCAG CTGCA^G
SacII 3097 CCGCGG CCGC^GG
SalI 1671 GTCGAC G^TCGAC
ScaI 1383 AGTACT AGT^ACT
SfoI 3356 GGCGCC GGC^GCC
SfuI 1553 TTCGAA TT^CGAA
SpeI 1621 ACTAGT A^CTAGT
SphI 3125 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1344 GTCTAC GT^[AC][GT]AC
1671 GTCGAC
AccII 3098 CGCG CG^CG
3272 CGCG
AflIII 976 ACATGT A^C[GA][CT]GT
1449 ACACGT
AseI 2365 ATTAAT AT^TAAT
3777 ATTAAT
AsnI 2365 ATTAAT AT^TAAT
3777 ATTAAT
AspI 958 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2239 GACAGTGTC
BbvCI 1191 CCTCAGC CCTCAGC(-5/-2)
2961 GCTGAGG
BsaWI 116 TCCGGT [AT]^CCGG[AT]
4154 ACCGGA
Bsp1407I 1883 TGTACA T^GTACA
2018 TGTACA
BspHI 1631 TCATGA T^CATGA
1796 TCATGA
BsrGI 1883 TGTACA T^GTACA
2018 TGTACA
BstUI 3098 CGCG CG^CG
3272 CGCG
BstXI 2145 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4295 CCAGCCCAGTGG
EcoNI 4048 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5310 CCTGCGAGAGG
EcoRV 529 GATATC GAT^ATC
2125 GATATC
HincII 1671 GTCGAC GT[CT]^[GA]AC
2902 GTCAAC
HindII 1671 GTCGAC GT[CT]^[GA]AC
2902 GTCAAC
MvnI 3098 CGCG CG^CG
3272 CGCG
PaeR7I 3688 CTCGAG C^TCGAG
3843 CTCGAG
PflFI 958 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2239 GACAGTGTC
PflMI 2573 CCATTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4143 CCACGGCCTGG
PshBI 2365 ATTAAT AT^TAAT
3777 ATTAAT
RcaI 1631 TCATGA T^CATGA
1796 TCATGA
SapI 4336 GAAGAGC GCTCTTC(1/4)
496 GCTCTTC
SexAI 2266 ACCAGGT A^CC[AT]GGT
5042 ACCTGGT
SspBI 1883 TGTACA T^GTACA
2018 TGTACA
Tth111I 958 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2239 GACAGTGTC
Van91I 2573 CCATTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4143 CCACGGCCTGG
XhoI 3688 CTCGAG C^TCGAG
3843 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI