Restriction map for
pF1KA0487
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DdeI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HaeIII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MscI MslI MunI NaeI
NarI NciI NcoI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 122 GGTACC G^GTACC
AcsI 456 AAATTC [GA]^AATT[CT]
AlwNI 296 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 456 AAATTC [GA]^AATT[CT]
Asp718I 122 GGTACC G^GTACC
AvaI 163 CCCGAG C^[CT]CG[GA]G
AvaII 29 GGACC G^G[AT]CC
BanI 122 GGTACC G^G[CT][GA]CC
BsgI 506 CTGCAC GTGCAG(16/14)
BsiEI 36 CGACCG CG[GA][CT]^CG
BsoBI 163 CCCGAG C^[CT]CG[GA]G
Bsp1407I 402 TGTACA T^GTACA
BsrFI 119 GCCGGT [GA]^CCGG[CT]
BsrGI 402 TGTACA T^GTACA
BstXI 127 CCAGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 119 GCCGGT [GA]^CCGG[CT]
Cfr13I 29 GGACC G^G[ACGT]CC
EarI 345 GAAGAG CTCTTC(1/4)
FauI 57 GCGGG CCCGC(4/6)
HgaI 24 GACGC GACGC(5/10)
HphI 108 GGTGA GGTGA(8/7)
KpnI 122 GGTACC GGTAC^C
Ksp632I 345 GAAGAG CTCTTC(1/4)
MseI 332 TTAA T^TAA
MspA1I 503 CCGCTG C[AC]G^C[GT]G
MwoI 74 GCTTCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 278 CATATG CA^TATG
NlaIII 406 CATG CATG^
NspI 405 ACATGC [GA]CATG^[CT]
PflMI 445 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 215 GAGTC GAGTC(4/5)
Sau96I 29 GGACC G^G[ACGT]CC
SfaNI 370 GATGC GCATC(5/9)
SfcI 461 CTACAG C^T[GA][CT]AG
SspBI 402 TGTACA T^GTACA
TfiI 325 GATTC G^A[AT]TC
Tru9I 332 TTAA T^TAA
Tsp45I 298 GTCAC ^GT[GC]AC
Tsp509I 457 AATT ^AATT
Van91I 445 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 26 CGCG CG^CG
40 CGCG
AccIII 77 TCCGGA T^CCGGA
477 TCCGGA
AfaI 123 GTAC GT^AC
403 GTAC
AluI 425 AGCT AG^CT
431 AGCT
BfaI 229 CTAG C^TAG
530 CTAG
BpmI 385 CTCCAG CTGGAG(16/14)
212 CTGGAG
BsaJI 144 CCCAGG C^C[ACGT][ACGT]GG
164 CCGAGG
BsaWI 77 TCCGGA [AT]^CCGG[AT]
477 TCCGGA
BseAI 77 TCCGGA T^CCGGA
477 TCCGGA
BsiYI 145 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
445 CCAGCCAATGG
BslI 145 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
445 CCAGCCAATGG
BsmFI 142 GTCCC GGGAC(10/14)
466 GTCCC
BspEI 77 TCCGGA T^CCGGA
477 TCCGGA
BsrI 132 CCAGT ACTGG(1/-1)
387 CCAGT
BssKI 145 CCAGG ^CC[ACGT]GG
175 CCTGG
BstNI 145 CCAGG CC^[AT]GG
175 CCTGG
BstUI 26 CGCG CG^CG
40 CGCG
EcoRII 145 CCAGG ^CC[AT]GG
175 CCTGG
HinfI 215 GAGTC G^A[ACGT]TC
325 GATTC
MaeI 229 CTAG C^TAG
530 CTAG
MaeIII 298 GTCAC ^GT[ACGT]AC
499 GTAAC
MroI 77 TCCGGA T^CCGGA
477 TCCGGA
MvaI 145 CCAGG CC^[AT]GG
175 CCTGG
MvnI 26 CGCG CG^CG
40 CGCG
RsaI 123 GTAC GT^AC
403 GTAC
ScrFI 145 CCAGG CC^[ACGT]GG
175 CCTGG
SspI 306 AATATT AAT^ATT
334 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BbvI BseRI Cac8I Fnu4HI
HapII HpaII ItaI MboII MnlI
MspI NlaIV TseI TspRI