Restriction map for
pF1KA0671
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BclI
BcnI BfaI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
HaeIII HapII HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 219 GAATTC [GA]^AATT[CT]
AcyI 1318 GACGCC G[GA]^CG[CT]C
AfaI 481 GTAC GT^AC
AflII 174 CTTAAG C^TTAAG
Alw44I 1482 GTGCAC G^TGCAC
AlwNI 710 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1482 GTGCAC G^TGCAC
ApoI 219 GAATTC [GA]^AATT[CT]
AseI 636 ATTAAT AT^TAAT
AsnI 636 ATTAAT AT^TAAT
AspHI 1482 GTGCAC G[AT]GC[AT]^C
AviII 1224 TGCGCA TGC^GCA
BbvCI 154 CCTCAGC CCTCAGC(-5/-2)
BbvI 1017 GCAGC GCAGC(8/12)
BfrI 174 CTTAAG C^TTAAG
BpmI 1031 CTGGAG CTGGAG(16/14)
BsaAI 1488 TACGTA [CT]AC^GT[GA]
BsaHI 1318 GACGCC G[GA]^CG[CT]C
BsgI 869 CTGCAC GTGCAG(16/14)
BsiHKAI 1482 GTGCAC G[AT]GC[AT]^C
Bsp1407I 480 TGTACA T^GTACA
BspHI 60 TCATGA T^CATGA
BspMI 1478 GCAGGT ACCTGC(4/8)
BsrDI 1409 CATTGC GCAATG(2/0)
BsrGI 480 TGTACA T^GTACA
BssSI 1105 CTCGTG CACGAG(-5/-1)
BstBI 213 TTCGAA TT^CGAA
BstF5I 408 GGATG GGATG(2/0)
DraI 1539 TTTAAA TTT^AAA
DraIII 987 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 355 CCATGG C^C[GA][CT]GG
EcoRI 219 GAATTC G^AATTC
EcoRV 1026 GATATC GAT^ATC
EcoT14I 355 CCATGG C^C[AT][AT]GG
FokI 408 GGATG GGATG(9/13)
FspI 1224 TGCGCA TGC^GCA
HaeII 461 GGCGCT [GA]GCGC^[CT]
Hin1I 1318 GACGCC G[GA]^CG[CT]C
MslI 1042 CATACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 355 CCATGG C^CATGG
NspV 213 TTCGAA TT^CGAA
PflMI 355 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 636 ATTAAT AT^TAAT
RcaI 60 TCATGA T^CATGA
RsaI 481 GTAC GT^AC
SfuI 213 TTCGAA TT^CGAA
SnaBI 1488 TACGTA TAC^GTA
SphI 1278 GCATGC GCATG^C
SspBI 480 TGTACA T^GTACA
SspI 184 AATATT AAT^ATT
StyI 355 CCATGG C^C[AT][AT]GG
TseI 1017 GCAGC G^C[AT]GC
Van91I 355 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1465 CGCG CG^CG
1467 CGCG
AvaII 898 GGACC G^G[AT]CC
1158 GGACC
BanII 720 GAGCCC G[GA]GC[CT]^C
1507 GGGCTC
BciVI 546 GGATAC GTATCC(6/5)
978 GGATAC
BsaJI 309 CCCAGG C^C[ACGT][ACGT]GG
355 CCATGG
BseRI 152 CTCCTC GAGGAG(10/8)
1344 CTCCTC
BsmAI 143 GAGAC GTCTC(1/5)
1034 GAGAC
BssKI 310 CCAGG ^CC[ACGT]GG
913 CCTGG
BstNI 310 CCAGG CC^[AT]GG
913 CCTGG
BstUI 1465 CGCG CG^CG
1467 CGCG
BstYI 1270 AGATCC [GA]^GATC[CT]
1380 AGATCC
Cfr13I 898 GGACC G^G[ACGT]CC
1158 GGACC
EcoRII 310 CCAGG ^CC[AT]GG
913 CCTGG
HgaI 1318 GACGC GACGC(5/10)
1020 GCGTC
MaeII 1204 ACGT A^CGT
1489 ACGT
MflI 1270 AGATCC [GA]^GATC[CT]
1380 AGATCC
MvaI 310 CCAGG CC^[AT]GG
913 CCTGG
MvnI 1465 CGCG CG^CG
1467 CGCG
NlaIV 897 GGGACC GG[ACGT]^[ACGT]CC
1508 GGCTCC
PstI 1450 CTGCAG CTGCA^G
1476 CTGCAG
Sau96I 898 GGACC G^G[ACGT]CC
1158 GGACC
ScrFI 310 CCAGG CC^[ACGT]GG
913 CCTGG
SfaNI 409 GATGC GCATC(5/9)
278 GCATC
SmlI 174 CTTAAG C^T[CT][GA]AG
643 CTTGAG
Tsp45I 965 GTGAC ^GT[GC]AC
1002 GTCAC
XhoII 1270 AGATCC [GA]^GATC[CT]
1380 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI Asp700I BmyI
BsiYI BslI BsmFI Bsp1286I BsrI
Cac8I CfoI DdeI DpnII EarI
Fnu4HI HhaI HinP1I HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII NspI
PleI Sau3AI SfcI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I XmnI