Restriction map for
pF1KA0715
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# NO CUT ENZYMES ( 67 enzymes) #
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Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AvrII BbeI BbrPI BfrI BglII
BlnI BsaAI BsiEI BsiWI BssHII
Bst1107I BstZ17I Bsu36I DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI HaeII
HindIII HpaI KasI KpnI KspI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII SacII SbfI SexAI
SfoI SgrAI SnaBI Sse8387I SspI
SwaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1845 GACGTC GACGT^C
AccIII 2546 TCCGGA T^CCGGA
AcyI 1845 GACGTC G[GA]^CG[CT]C
Alw44I 2182 GTGCAC G^TGCAC
ApaI 1466 GGGCCC GGGCC^C
ApaLI 2182 GTGCAC G^TGCAC
AseI 2425 ATTAAT AT^TAAT
AsnI 2425 ATTAAT AT^TAAT
AviII 2501 TGCGCA TGC^GCA
BcgI 878 CGAAAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3802 GTATCC GTATCC(6/5)
BglI 1957 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1845 GACGTC G[GA]^CG[CT]C
BsaWI 2546 TCCGGA [AT]^CCGG[AT]
BseAI 2546 TCCGGA T^CCGGA
BsmBI 874 GAGACG CGTCTC(1/5)
Bsp1407I 3812 TGTACA T^GTACA
BspDI 2992 ATCGAT AT^CGAT
BspEI 2546 TCCGGA T^CCGGA
BspLU11I 1097 ACATGT A^CATGT
BsrGI 3812 TGTACA T^GTACA
BssSI 3295 CTCGTG CACGAG(-5/-1)
BstEII 1812 GGTCACC G^GT[ACGT]ACC
BstPI 1812 GGTCACC G^GT[ACGT]ACC
ClaI 2992 ATCGAT AT^CGAT
CpoI 3067 CGGTCCG CG^G[AT]CCG
EcoO65I 1812 GGTCACC G^GT[ACGT]ACC
FspI 2501 TGCGCA TGC^GCA
Hin1I 1845 GACGTC G[GA]^CG[CT]C
MfeI 2859 CAATTG C^AATTG
MroI 2546 TCCGGA T^CCGGA
MunI 2859 CAATTG C^AATTG
NcoI 326 CCATGG C^CATGG
PshAI 2760 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2425 ATTAAT AT^TAAT
RsrII 3067 CGGTCCG CG^G[AT]CCG
SacI 4149 GAGCTC GAGCT^C
SalI 3576 GTCGAC G^TCGAC
ScaI 4188 AGTACT AGT^ACT
SfiI 1956 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1532 CCCGGG CCC^GGG
SpeI 2211 ACTAGT A^CTAGT
SphI 3230 GCATGC GCATG^C
SspBI 3812 TGTACA T^GTACA
XbaI 2475 TCTAGA T^CTAGA
XmaCI 1532 CCCGGG C^CCGGG
XmaI 1532 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 2359 GTCTAC GT^[AC][GT]AC
3576 GTCGAC
AccII 3139 CGCG CG^CG
3320 CGCG
AcsI 458 GAATTT [GA]^AATT[CT]
745 AAATTT
ApoI 458 GAATTT [GA]^AATT[CT]
745 AAATTT
Asp700I 657 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2683 GAAGGAGTTC
BclI 389 TGATCA T^GATCA
1112 TGATCA
BlpI 1381 GCTAAGC GC^T[ACGT]AGC
1504 GCTCAGC
Bpu1102I 1381 GCTAAGC GC^T[ACGT]AGC
1504 GCTCAGC
BsaBI 966 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1134 GATCCCCATC
BsmI 3009 GAATGC GAATGC(1/-1)
3604 GCATTC
BspHI 386 TCATGA T^CATGA
3143 TCATGA
BsrBI 3084 CCGCTC CCGCTC(-3/-3)
928 GAGCGG
BstAPI 2504 GCACACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4211 GCACGGAGTGC
BstBI 455 TTCGAA TT^CGAA
685 TTCGAA
BstUI 3139 CGCG CG^CG
3320 CGCG
CelII 1381 GCTAAGC GC^T[ACGT]AGC
1504 GCTCAGC
DraIII 4099 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4212 CACGGAGTG
DsaI 326 CCATGG C^C[GA][CT]GG
3759 CCACGG
EcoNI 1119 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2247 CCTGCCCCAGG
FbaI 389 TGATCA T^GATCA
1112 TGATCA
HgaI 3140 GCGTC GACGC(5/10)
3827 GCGTC
MamI 966 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1134 GATCCCCATC
MvnI 3139 CGCG CG^CG
3320 CGCG
NspV 455 TTCGAA TT^CGAA
685 TTCGAA
RcaI 386 TCATGA T^CATGA
3143 TCATGA
SfuI 455 TTCGAA TT^CGAA
685 TTCGAA
StuI 2134 AGGCCT AGG^CCT
4060 AGGCCT
XcmI 353 CCATGTTACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
774 CCAGACCAGGACTGG
XmnI 657 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2683 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII